Summary
Using relatively high dilutions of anti-Leu 7 monoclonal antibody and a four-step peroxidase-antiperoxidase (PAP) reaction in paraffin-embedded tissues, we tested the affinity of this antibody to the cells of 47 human nerve sheath tumors and 22 other tumors in which the differential diagnosis with nerve sheath neoplasms is known to arise. Of all the nerve sheath tumors studied 68%, including 80% of the schwannomas, contained anti-Leu 7-positive cells. All 22 non-schwannian neoplasms were entirely, negative. Specimens of eight experimental malignant rat schwannomas were also negative for anti-Leu 7 antibody. Our findings suggest that anti-Leu 7 monoclonal antibody is a promising marker that may facilitate the differential diagnosis between human Schwann cell and non-schwann cell neoplasms.
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Supported by a grant from the Fondation Suisse de Bourses de Médecine et Biologic (EP) and by research grant no. CA 31271 from the National Cancer Institute, US Dept. of Health and Human Services (LJR)
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Perentes, E., Rubinstein, L.J. Immunohistochemical recognition of human nerve sheath tumors by anti-Leu 7 (HNK-1) monoclonal antibody. Acta Neuropathol 68, 319–324 (1985). https://doi.org/10.1007/BF00690835
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DOI: https://doi.org/10.1007/BF00690835