Summary
A monoclonal antibody (Am-3) was produced against senile plaques in the brain of a patient with Alzheimer's disease. Am-3 was reactive with senile plaques of typical, primitive and diffuse type not only in the brain used as immunogen, but also those in the brain of 15 out of 25 autopsy cases of the aged people. Moreover, Am-3 was also reactive with granular materials of various sizes scattered in the 1st, 3rd and 4th layers of the cerebral cortices of the cases with severe dementia. Am-3 was also reactive with vessel wall of the congophilic angiopathy. By immunoelectron microscopic examination, Am-3 was positive with amyloid fibril in the core and crown of senile plaques, and in the congophilic angiopathy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Davies L, Wolska B, (1988) A4 amyloid protein deposition and the diagnosis of Alzheimer's disease. Neurology 38:1688–1693
Glenner GC, Wong CW (1984) Alzheimer's disease and Down's syndrome: sharing of a unique cerebrovascular amyloid fibril protein. Biochem Biophys Res Commun 120:885–890
Goldbager D, Lerman MI, McBride OW, Saffiotti U, Gajdusek DC (1987) Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer's disease. Science 235:877–880
Hirokawa K, Utsuyama M, Moriizumi E, Handa S (1986) Analysis of the thymic microenvironment by monoclonal antibodies with special reference to thymic nurse cells. Thymus 8:349–360
Kang J, Lemaire H-G, Unterbeck A, Salbaum JM, Master CL, Grzeschik KH, Multhaup G, Beyreuther K, Muller-Hill B (1987) The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature 325:733–736
Kitamoto T, Ogomori K, Tateishi J, Pruisner SB (1987) Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab Invest 57:230–236
Masters CL, Simms M, Weinman NA, Multhaup G, McDonald BL, Beyreuther K (1985) Amyloid plaque core protein in Alzheimer's disease and Down's syndrome. Proc Natl Acad Sci USA 82:8729–8732
Masters CL, Multhaup G, Simms G, Pottgieser J, Martins RN, Beyreuther K (1985) Neuronal origin of a cerebral amyloid: neurofibrillary tangles of Alzheimer's disease contain the same protein as the amyloid of plaque cores and blood vessels. EMBO J 4:1757–1763
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative: a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Tanzi RE, Gusella JF, Watkins PC, Bruns GAP, George-Hyslop PS, Van Keuren ML, Patterson D, Pagan S, Kurnit DM, Neve RL (1987) Amyloid beta protein: cDNA, mRNA, distribution, and genetic linkage near the Alzheimer locus. Science 235:880–884
Yamaguchi H, Hirai S, Morimatsu M, Shoji M, Ihara Y (1988). A variety of cerebral amyloid deposits in the brains of the Alzheimer-type dementia demonstrated by beta protein immunostaining. Acta Neuropathol 76:541–549
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kawasaki, H., Utsuyama, M., Takahashi, H. et al. Establishment of a monoclonal antibody against senile plaques and its application for immunohistological and immunoelectron microscopical studies in the brain of the elderly. Acta Neuropathol 79, 44–47 (1989). https://doi.org/10.1007/BF00308956
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00308956