Summary
Gene delection of chromosome 10 in 11 tumor tissues removed from nine patients with malignant glioma (seven glioblastomas and two malignant astrocytomas) was investigated. Loss of heterozygosity was found in four of the seven (57%) cases with glioblastoma, whereas heterozygosity was preserved in two malignant astrocytome cases. One glioblastoma case showed loss of heterozygosity only in the recurring tumor tissue, although heterozygosity was maintained in the initial tumor tissue. The significance of loss of heterozygosity in the growth and recurrence of glioblastoma is discussed.
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References
Bigner SH, Mark J, Burger PC, Mahaley MS, Bulleard DE, Muhlbaier LH, Bigner DD (1988) Specific chromosomal abnormalities in malignant human gliomas. Cancer Res 48:405–411
Birchmeier C, Sharma S, Wigler M (1987) Expression and rearrangement of the ROS I gene in human glioblastoma cells. Proc Natl Acad Sci USA 84:9270–9274
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Friend SH, Bernards R, Rogelj S, Weinberg RA, Rapaport JM, Albert DM, Dryja TP (1986) A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature 323:643–646
Fujimoto M, Sheridan PJ, Sharp ZD, Weaker FJ, Kagan-Hallet KS, Story JL (1989) Proto-oncogene analysis in brain tumors. J Neurosurg 70:910–915
Garson JA, McIntyre PG, Kemshead JT (1985) N-myc amplification in malignant astrocytoma. Lancet II:718–719
Hoff M, Nakamura Y, Payson R, O'Connell P, Leppert M, Lathrop GM, Lalouel J-M, White R (1988) Isolation an mapping of a polymorphic DNA sequence pMHZ 15 on chromosome 10 [D10S17]. Nucleic Acids Res 16:373
James CD, Carlbom E, Dumanski JP, Hansen M, Nordenskjold M, Collins VP, Cavenee WK (1988) Clonal genomic alterations in glioma malignancy stages. Cancer Res 48:5546–5551
Klein G (1987) The approaching era of the tumor suppressor genes. Science 238:1539–1545
Kinzler KW, Bigner SH, Bigner DD, Trent JM, Law ML, O'Brien SJ, Wong AJ, Vogelstein B (1987) Identification of an amplified, highly expressed gene in a human glioma. Science 236:70–73
Libermann TA, Nusbaum HR, Razon N, Kris R, Lax I, Soreq H, Whittle N, Watterfield MD, Ullrich A, Schlessinger J (1985) Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumors of glial origin. Nature 313:144–147
Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, White R (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235:1616–1622
Nister M, Helding C-H, Westermark B (1986) Clonal variation in the production of a platelet-derived growth factor-like protein and expression of corresponding receptors in a human malignant glioma. Cancer Res 46:332–340
Perucho M, Goldfarb M, Shimizu K, Lama C, Fogh J, Wigler M (1981) Human tumor-derived cell lines contain common and different transforming genes. Cell 27:467–476
Schmidek HH (1987) The molecular genetics of nervous system tumors. J Neurosurg 67:1–16
Shapiro S, Mealey J Jr, Sartorius C (1989) Radiation-induced intracranial malignant gliomas. J Neurosurg 71:77–82
Southern EM (1975) Detection of specific sequences among DNA fragments separeted by gel electrophoresis. J Mol Biol 98:503–517
Trent J, Meltzer P, Rosenblum M, Harsh G, Kinzler K, Mashhal R, Feinberg A, Vogelstein B (1986) Evidence for rearrangement, amplification, and expression of c-myc in a human glioblastoma. Proc Natl Acad Sci USA 83:470–473
Verde P, Stoppelli MP, Galeffi P, DiNocera P, Blasi F (1984) Identification and primary sequence of an unspliced human urokinase poly(A)+ RNA. Proc Natl Acad Sci USA 81:4727–4731
Weinberg RA (1985) The action of oncogenes in the cytoplasm and nucleus. Science 230:770–776
Whittle IR (1989) Oncogenes and neuro-oncology. Br J Neurosurg 3:3–12
Zülch KJ (1979) Histological typing of tumours of the central nervous system. International Histological Classification of Tumors, No 21. World Health Organization, Geneva
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Watanabe, K., Nagai, M., Wakai, S. et al. Loss of constitutional heterozygosity in chromosome 10 in human glioblastoma. Acta Neuropathol 80, 251–254 (1990). https://doi.org/10.1007/BF00294641
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DOI: https://doi.org/10.1007/BF00294641