Summary
To clarify the relationship between the percentage of c-myc protein-labeled cells, the bromodeo-xyuridine (BrdUrd) labeling index (LI) and clinical malignancy in pituitary adenomas, we studied 31 cases of pituitary adenomas. Tumor invasiveness, recurrence, tumor size and the length of illness were evaluated from operative findings, magnetic resonance imaging findings, and the clinical course. Each pituitary adenoma was scored to represent the degree of clinical malignancy. An hour before excision of the tumor, we administered BrdUrd intravenously. Surgical materials were fixed in 70% alcohol and embedded in paraffin. Both hematoxylin and eosin staining and immunohistochemical staining were performed using a monoclonal antibody for both anti-BrdUrd and anti-c-myc protein. Among pituitary adenomas, there was a significantly low percentage of c-myc protein-labeled cells in cases with acromegaly. The percentage of c-myc protein-labeled cells in the pituitary adenomas tended to increase with increase with the total scores of clinical malignancy. The BrdUrd LI was lower than 1% in almost all cases of pituitary adenomas, and it showed no correlation with their clinical malignancy. In conclusion, determination of the percentage of c-myc protein-labeled cells in pituitary adenomas proved to be useful for evaluating their clinical malignancy.
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References
Anniko M, Tribukait B, Wersall J (1984) DNA ploidy and cell phase in human pituitary tumors. Cancer 53:1708–1713
Donner P, Greiser-Wilke I, Moelling K (1982) Nuclear localization and DNA binding of the transforming gene product of avian myelocytomatosis virus. Nature 296:262–264
Goto K, Ogo A, Yanase T, Hagi M, Ohashi M, Nawata H (1990) Expression of c-fos and c-myc proto-oncogenes in human adrenal pheochromocytomas. J Clin Endocrinol Metab 70:353–357
Gratzer HG (1982) Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218:474–476
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Iguchi-Ariga SMM, Itani T, Kiji Y, Ariga H (1987) Possible function of the c-myc product: promotion of cellular DNA replication. EMBO J 6:2365–2371
Iguchi-Agriga SMM, Itani T, Yamaguchi M, Ariga H (1987) c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication. Nucleic Acids Res 15:4889–4899
Ikeda H, Yoshimoto T (1991) Developmental changes in proliferative activity of cells of the murine Rathke's pouch. Cell Tissue Res 263:41–47
Kelly K, Cochran BH, Stiles CD, Leder P (1983) Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell 35:603–6105
Land H, Parada LF, Weinberg RA (1983) Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature 304:596–602
Landolt AM, Shibata T, Kleihues P (1987) Growth rate of human pituitary adenomas. J Neurosurg 67:803–806
Ludecke DK, Beck-Bornholdt HP, Saeger W, Schmidt (1985) Tumor ploidy in DNA histograms of pituitary adenomas. Acta Neurochir (Wien) 76:18–22
Martins AN, Hayes GJ, Kempe LG (1965) Invasive pituitary adenomas. J Neurosurg 22:268–276
McComb DJ, Kovacs K (1978) Ultrastructural morphometry of sparsely granulated prolactin cell adenomas of the human pituitary. Acta endocrinol (Copenh) 89:521–529
Persson H, Leder P (1984) Nuclear localization and DNA binding properties of a protein expressed by human c-myc oncogene. Science 225:718–720
Pfeifer-Ohlsson S, Goustin AS, Rydnet J, Wahlstoem T, Bjersing L, Stehelin D, Ohlsson R (1984) Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta. Implications for embryonic cell proliferation. Cell 35:585–596
Reynold RK, Hoekzema GS, Vogel J, Hinrichs SH, Jay G (1988) Multiple endocrine neoplasia induced by the promiscuous expression of a viral oncogene. Proc Natl Acad Sci USA 85:3135–3139
Slamon DJ, Cline MJ (1984) Expression of cellular oncogenes during embryonic and fetal development of the mouse. Proc Natl Acad Sci USA 81:7141–7145
Solitare GB, Jatlow P (1967) Adenohypophysial carcinoma. J Neurosurg 26:624–632
Studzinski S, Brelvi ZS, Feldman SC, Watt RA (1986) Participation of c-myc protein in DNA synthesis of human cells. Science 234:467–470
Sugano S, Yamaguch N (1984) Two cases of transformation-deficient, immortalization-positive simian virus 40 mutants constructed by making three-base insertions in the T antigen gene. J Virol 52:884–891
Varumus HE (1987) Oncogenes and transcriptional control. Science 238:1337–1339
Weinberg RA (1985) The action of oncogenes in the cytoplasm and nucleus. Science 230:770–776
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Ikeda, H., Yoshimoto, T. The relationship between c-myc protein expression, the bromodeoxyuridine labeling index and the biological behavior of pituitary adenomas. Acta Neuropathol 83, 361–364 (1992). https://doi.org/10.1007/BF00713526
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DOI: https://doi.org/10.1007/BF00713526