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A continuous cell line (KK-2) from a supratentorial primitive neuroectodermal tumor

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Summary

Tumor tissue located in the occipital lobe with hemorrhage was obtained from a 19-year-old patient. Histological examination indicated it to consist of undifferentiated small, round cells without neuronal or glial differentiation, and possibly to be a type of primitive neuroectodermal tumor. The tumor cells were cultured for 3 years and a continuous cell line (KK-2) was established. KK-2 was transplantable to nude mice. With immunocytochemistry, neuron-specific enolase, protein gene product 9.5, vimentin, TUJ1 (a monoclonal antibody specific for neuron-associated class III β-tubulin isotype) and 6H7 (a monoclonal antibody to NCAM produced by us) were detected. None of the following could be found: glial fibrillary acidic protein, S-100 protein, neurofilament and synaptophysin, calcitonin gene-related peptide, gastrin releasing peptide corticotropin-releasing factor, substance P, somatostatin, chromogranin, aromatic l-amino acid decarboxylase and tyrosine hydroxylase. The original tumor and KK-2 cells obtained after 3 years of culture and transplants in nude mice displayed essentially the same ultrastructural and immunohistochemical characteristics. KK-2 cells showed no differentiation to mature neuronal, glial or ependymal cells. This cell line may possibly serve as a useful model for studying cellular differentiation of human neuroectodermal tumors and normal neuronal development.

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Authors and Affiliations

  1. Department of Pathology, Kitasato University School of Medicine, 228, Sagamihara, Kanagawa, Japan

    H. Ito & T. Kameya

  2. Department of Neurosurgery, Kitasato University School of Medicine, 228, Sagamihara, Kanagawa, Japan

    H. Ito, T. Suwa & N. Kawano

  3. Department of Clinical Pathology, Kitasato University School of Medicine, 228, Sagamihara, Kanagawa, Japan

    C. Wada

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  1. H. Ito
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  2. T. Kameya
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  3. T. Suwa
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  4. C. Wada
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  5. N. Kawano
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Supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare (1–5), Japan

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Ito, H., Kameya, T., Suwa, T. et al. A continuous cell line (KK-2) from a supratentorial primitive neuroectodermal tumor. Acta Neuropathol 84, 52–58 (1992). https://doi.org/10.1007/BF00427215

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  • DOI: https://doi.org/10.1007/BF00427215

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