Abstract
We report the pathological changes in skeletal muscle from a patient with acute adult T cell leukemia (ATL). HTLV-I provirus was detected in infiltrating cells using in situ polymerase chain reaction in frozen sections. Furthermore, aberrant expression of the p53 protein was observed in the infiltrating cells. As p53 protein was not observed in mononuclear inflammatory cells in patients with polymyositis, expression of the p53 protein was considered to be one of the characteristic findings in ATL cells. This is the first direct detection of ATL cells in skeletal muscle.
Similar content being viewed by others
References
Arai E, Chow KC, Li CY, Tokunaga M, Katayama I (1994) Differentiation between cutaneous form of adult T cell leukemia/lymphoma and cutaneous T cell lymphoma by in situ hybridization using a human T cell leukemia virus-1 DNA probe. Am J Pathol 144: 15–20
Dubowitz V, Brooke MH (1973) Muscle biopsy—a modern approach. Saunders, London
Embretson J, Zupancic M, Ribas JL, Burke A, Racz P, Tenner-Racz K, Haase AT (1993) Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period of AIDS. Nature 362: 359–362
Finlay CA, Hinds PW, Levine AJ (1989) The p53 proto-oncogene can act as a suppressor of transformation. Cell 57: 1083–1093
Franchini G, Wong-Staal F, Gallo RC (1984) Human T cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T cell leukemia. Proc Natl Acad Sci USA 81: 6207–6211
Gabbai AA, Wiley CA, Oliveira ASB, Smith R, Schmidt B, Nóbrega JAM, Bordin JO, Román GC (1994) Skeletal muscle involvement in tropical spastic paraparesis/HTLV-I-associated myelopathy. Muscle Nerve 17: 923–930
Higuchi I, Nerenberg M, Yoshimine K, Yoshida M, Fukunaga H, Tajima K, Osame M (1992) Failure to detect HTLV-I by in situ hybridization in the biopsied muscles of viral carriers with polymyositis. Muscle Nerve 15: 43–47
Higuchi I, Montemayor E, Izumo S, Inose M, Osame M (1993) Immunohistochemical characteristics of polymyositis in patients with HTLV-I-associated myelopathy and HTLV-I carriers. Muscle Nerve 16: 472–476
Hinds P, Finlay C, Levine AJ (1989) Mutation is required to activate the p53 gene for cooperation with the ras oncogene and transformation. J Virol 63: 739–746
Ishii K, Yamato K, Iwahara Y, Eguchi T, Uemura Y, Takehara N, Ohtsuki Y, Taguchi H, Miyoshi I (1991) Isolation of HTLV-I from muscle of a patient with polymyositis. Am J Med 90: 267–269
Kinoshita T, Shimoyama M, Tobinai K, Ito M, Ito S, Ikeda S, Tajima K, Shimotohno K, Sugimura T (1989) Detection of mRNA for the tax 1/rex 1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction. Proc Natl Acad Sci USA 86: 5620–5624
Lane DP, Crawford LV (1979) T antigen is bound to a host protein in SV40 transformed cells. Nature 278: 261–263
Leon-Monzon M, Illa I, Dalakas MC (1994) Polymyositis in patients infected with human T-cell leukemia virus type I: the role of the virus in the cause of the disease. Ann Neurol 36: 643–649
Long AA, Komminoth P, Lee E, Wolfe HJ (1993) Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections. Detection of viral DNA, gene rearrangements and chromosomal translocations. Histochemistry 99: 151–162
Nagai H, Kinoshita T, Imamura J, Murakami Y, Hayashi K, Mukai K, Ikeda S, Tobinai K, Saito H, Shimoyama M, Shimotohno K (1991) Genetic alteration of p53 in some patients with adult T-cell leukemia. Jpn J Cancer Res 82: 1421–1427
Nigro JM, Baker SJ, Preisinger AC, Jessup JM, Hostetter R, Cleary K, Bigner SH, Davidson N, Baylin S, Devilee P, Glover T, Collins FS, Weston A, Modali R, Harris CC, Vogelstein B (1989) Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705–708
Nuovo GJ (1994) PCR in situ hybridization. Protocols and applications, 2nd edn. Raven Press, New York
Nuovo GJ, Gallery F, MacConnell P, Delvenne P (1991) An improved technique for the in situ detection of DNA after polymerase chain reaction amplification. Am J Pathol 139: 1239–1244
Nuovo GJ, Margiotta M, MacConnell P, Becker J (1992) Rapid in situ detection of PCR-amplified HIV-1 DNA. Diagn Mol Pathol 1: 98–102
Poiesz BJ, Ruscetti FW, Gazdar AF, Bunn PA, Minna JD, Gallo RC (1980) Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T cell lymphoma. Proc Natl Acad Sci USA 77: 7415–7419
Sakashita A, Hattori T, Miller CW, Suzushima H, Asou N, Takatsuki K, Koeffler HP (1992) Mutations of the p53 gene in adult T-cell leukemia. Blood 79: 477–480
Shimamoto Y, Suga K, Shibata K, Matsuzaki M, Yano H, Yamaguchi M (1994) Clinical importance of extraordinary integration patterns of human T cell lymphotrophic virus type I proviral DNA in adult T cell leukemia/lymphoma. Blood 84: 853–858
Sueyoshi K, Goto M, Johnosono M, Sato E, Shibata D (1994) Anatomical distribution of HTLV-I proviral sequence in an autopsy case of HTLV-I associated myelopathy: a polymerase chain reaction study. Pathol Int 44: 27–33
Sugito S, Yamato K, Sameshima Y, Yokota J, Yano S, Miyoshi I (1991) Adult T cell leukemia: structures and expression of the p53 gene. Int J Cancer 49: 880–885
Uchiyama T, Yodoi J, Segawa K, Takatsuki K, Uchino H (1977) Adult T cell leukemia: clinical and hematological features of 16 cases. Blood 50: 481–492
Wiley CA, Nerenberg M, Cros D, Soto-Aguilar MC (1989) HTLV-I polymyositis in a patient also infected with the human immunodeficiency virus. N Engl J Med 320: 992–995
Yamato K, Oka T, Hiroi M, Iwahara Y, Sugito S, Tsuchida N, Miyoshi I (1993) Aberrant expression of the p53 tumor suppressor gene in adult T cell leukemia and HTLV-I-infected cells. Jpn J Cancer Res 84: 4–8
Yoshida M (1987) Expression of the HTLV-I genome and its association with a unique T cell malignancy. Biochim Biophys Acta 907: 145–161
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Higuchi, I., Hashimoto, K., Kashio, N. et al. In situ polymerase chain reaction detection of HTLV-I provirus and expression of the p53 tumor suppressor gene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia. Acta Neuropathol 90, 323–327 (1995). https://doi.org/10.1007/BF00296518
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00296518