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Differential analysis of experimental hypermelanosis induced by UVB, PUVA, and allergic contact dermatitis using a brownish guinea pig model

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Summary

In moderately colored guinea-pig skin, UVB, PUVA, and allergic contact dermatitis were shown to induce hyperpigmentation that resembled the pigmentary changes observed in mongoloid human skin. Using this model, we examined the effects of chemical agents, including tyrosinase inhibitors and sunscreen agents, on the color changes induced by UV irradiation. The daily exposure of brownish guinea-pig skin to UVB irradiation at a variety of energies for 3 successive days induced clearly visible black pigmentation on the irradiated rectangular areas of the flank within a few days of irradiation, the maximum being reached about 1 week after irradiation, i.e., similar to the changes that occur in pigmented human skin. Split epidermal sheets prepared from untreated pigmented guinea pigs exhibited 200–400 melanocytes/mm2; 1 week after UV irradiation, the applied areas show an increased number of strongly dopa-positive melanocytes with stout dendrites (800–1,000 cells/mm2). UVA irradiation following an intraperitoneal injection of 8-methoxypsoralen (8-MOP) also produced black pigmentation 1 week after irradiation, and this was paralleled by a marked increase in the number of dopa-positive melanocytes in dopa-reacted split epidermal sheets. Allergic contact dermatitis produced by the application of 1-phenylazo-2-naphtol induced hyperpigmentation after an interval of about 14 days in 10 of the 21 allergy-acquiring animals examined. This induced pigmentation was accompanied by an increase in the number of dopa-positive melanocytes as compared to the number seen in controls. In contrast, allergic contact dermatitis produced by the application of dinitrochlorobenzene failed to induce such a high ratio of postpigmentation, with only 3 of the 21 allergy-acquiring animals showing hyperpigmentation and 5 showing depigmentation; in the latter, there was a slight decrease in the number of dopa-positive melanocytes. To study the preventive effect of tyrosine inhibitors on UVB-induced pigmentation, daily topical applications of these compounds were performed after three daily UVB irradiations. Treatment with 10% hydroquinone for 10 days interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to the number found in UVB-irradiated, untreated control skin.

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References

  1. Bleehem SS (1981) Vitiligo-like leukoderma produced by substituted phenols. In: Seiji M (ed) Pigment cell, vol 6. Karger, Basel, pp 461–466

    Google Scholar 

  2. Bleehem SS, Pathak MA, Hori Y, Fitzpatrick TB (1968) Depigmentation of skin with 4-isopropylcatechol, mercaptoamines and other compounds. J Invest Dermatol 50:103–117

    Google Scholar 

  3. Blog FB, Szabo G (1979) The effects of psoralen and UVA (PUVA) on epidermal melanocytes of the tail in C57 BL mice. J Invest Dermatol 73:533–537

    Google Scholar 

  4. Blog FB, Szabo G (1979) The effects of UVB and 7,12,-dimethylbenzanthracene (DMBA) on epidermal melanocytes of the tail in C57 BL mice. J Invest Dermatol 73:538–544

    Google Scholar 

  5. Eisinger M, Marko O (1982) Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin. Proc Natl Acad Sci USA 79:2018–2022

    Google Scholar 

  6. Erickson KL, Montagna W (1975) The induction of melanogenesis by ultraviolet light in the pigmentary system of rhesus monkeys. J Invest Dermatol 65:279–284

    Google Scholar 

  7. Frenk E, Ott F (1971) Evaluation of the toxicity of the monoethyl ether of hydroquinone for mammalian melanocytes and melanoma cells. J Invest Dermatol 56:287–293

    Google Scholar 

  8. Gellin GA, Possick PA, Perone VB (1970) Depigmentation from 4-tertiary butyl catechol — an experimental study. J Invest Dermatol 55:190–197

    Google Scholar 

  9. Hashimoto A, Ithihashi M, Mishima Y (1984) The mechanism of depigmentation by hydroquinone: A study on suppression and recovery process of tyrosinase activity in pigment cells in vivo and in vitro. Jpn J Dermatol 94:797–804

    Google Scholar 

  10. Horio T, Okamoto H (1982) The mechanism of inhibitory effect of 8-methoxypsoralen and long-wave ultraviolet light on experimental contact sensitization. J Invest Dermatol 78:402–405

    Google Scholar 

  11. Hoshino S, Nishimura M, Fukuyama F, Gellin GA, Epstein JH (1981) Effects of 4-tertiary butyl catechol on melanocytes of hairless mice. J Invest Dermatol 76:231–238

    Google Scholar 

  12. Imokawa G, Mishima Y (1982) Loss of melanogenic properties in tyrosinase induced by glycosylation inhibitor within malignant melanoma cells. Cancer Res 42:1994–2002

    Google Scholar 

  13. Jimbow K, Obata H, Pathak MA, Fitzpatrick TB (1974) Mechanism of depigmentation by hydroquinone. J Invest Dermatol 62:436–449

    Google Scholar 

  14. Jimbow K, Uesugi T (1982) New melanogenesis and photobiological process in activation and proliferation of precursor melanocytes after UV exposure: Ultrastructural differentiation of precursor melanocytes from Langerhans cells. J Invest Dermatol 78:108–115

    Google Scholar 

  15. Kanerva L, Niemi KM, Lassus A (1981) Hyperpigmentation and hypopigmentation of the skin after long-term PUVA therapy. J Cutan Pathol 9:199–213

    Google Scholar 

  16. Kozuka T, Tashiro M, Sano S,Fujimoto K, Nakamura Y, Hashimoto S, Nakaminami G (1980) Pigmented contact dermatitis from azo dyes. I. Cross sensitivity in humans. Contact Dermatitis 6:330–336

    Google Scholar 

  17. Miller RA (1983) Psoralens and UVA-induced stellate hyperpigmented freckling. Arch Dermatol 80:306–308

    Google Scholar 

  18. Mishima Y, Widlan S (1967) Enzymically active and inactive melanocyte populations and ultraviolet irradiation: Combined dopa-premelanin reaction and electron microscopy. J Invest Dermatol 49:273–276

    Google Scholar 

  19. Mishima Y, Imokawa G (1983) Selective aberration and pigment loss in melanosomes of malignant melanoma cells in vitro by glycosylation inhibitors: Premelanosomes as glycoprotein. J Invest Dermatol 81:106–114

    Google Scholar 

  20. Nazzaro-Porro M, Passi S (1978) Identification of tyrosinase inhibitors in cultures of pityrosporum. J Invest Dermatol 71:205–208

    Google Scholar 

  21. Nordlund JJ, Ackles AE, Lerner AB (1981) The effect of ultraviolet light and certain drugs on La-bearing Langerhans cells in murine epidermis. Cell Immunol 60:50–63

    Google Scholar 

  22. Nordlund JJ, Ackles AE, Traynor FF (1981) The proliferative and toxic effects of ultraviolet light and inflammation on epidermal pigment cells. J Invest Dermatol 77:361–368

    Google Scholar 

  23. Okazaki K, Uzuka M, Morikawa F, Toda K, Seiji M (1976) Transfer mechanism of melanosomes in epidermal cell culture. J Invest Dermatol 67:541–547

    Google Scholar 

  24. Orthonne JP, Macdonald DM, Micoud A, Thivolet J (1979) PUVA-induced repigmentation of vitiligo: a histochemical (split-DOPA) and ultrastructural study. Br J Dermatol 101:1–12

    Google Scholar 

  25. Pathak MA, Jimbow K, Parrish JA (1976) Effect of UVA, UVB and psoralen on in vivo human melanin pigmentation. Pigm Cell 3:291–298

    Google Scholar 

  26. Rosdahl IK (1979) Local and systemic effects on the epidermal melanocyte population in UV-irradiated mouse skin. J Invest Dermatol 73:306–309

    Google Scholar 

  27. Rosdahl IK, Szabo G (1978) Mitotic activity of epidermal melanocytes in UV-irradiated mouse skin. J Invest Dermatol 70:143–148

    Google Scholar 

  28. Sato T, Kawada A (1972) Uptake of tritiated thymidine by epidermal melanocytes of hairless mice during ultraviolet light irradiation. J Invest Dermatol 58:71–73

    Google Scholar 

  29. Tsuchiya S, Kondo M, Okamoto K, Takase Y (1982) Studies on contact hypersensitivity in the guinea pig: The cumulative contact enhancement test. Contact Dermatitis 8:246–255

    Google Scholar 

  30. Tsuji T, Karasek M (1983) A procedure for the isolation of primary cultures of melanocytes from newborn and adult human skin. J Invest Dermatol 81:179–180

    Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, Kobe University School of Medicine, Kobe, Japan

    G. Imokawa, M. Kawai & Y. Mishima

  2. Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Tochigi 321-34, Japan

    I. Motegi

Authors
  1. G. Imokawa
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  2. M. Kawai
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  3. Y. Mishima
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  4. I. Motegi
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Imokawa, G., Kawai, M., Mishima, Y. et al. Differential analysis of experimental hypermelanosis induced by UVB, PUVA, and allergic contact dermatitis using a brownish guinea pig model. Arch Dermatol Res 278, 352–362 (1986). https://doi.org/10.1007/BF00418162

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  • DOI: https://doi.org/10.1007/BF00418162

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