Summary
The pattern of serine-specific tRNAs from guinea pig epidermis was determined and compared to that from liver by employing reversed phase chromatography on total tRNA preparations aminoacylated with 3H- or 14C-serine. Five tRNA sers (I–V) were found, two of which, tRNA serI and tRNA serV , appear to be “typical” for epidermis and in so far probably reflect in some way metabolic pecularities of epidermal cell differentiation. This hypothesis was further corroborated by showing that the locally applied corticosteroid triamcinolon selectively increases tRNA serI and tRNA serV 2-3-fold. The implications of these findings were discussed in detail.
Zusammenfassung
Durch „reversed phase”-Chromatographie von 3H- oder 14C-serinbeladenen Gesamt-tRNA-Präparaten wurde das Multiplizitätsmuster der serinspezifischen Transfer-Ribonucleinsäuren in der Meerschweinchenepidermis ermittelt und mit dem der Leber als Bezugssystem verglichen. Fünf tRNAser (I–V) konnten dargestellt werden, von denen zwei, tRNA serI und tRNA serV , „typisch” für die Epidermis zu sein scheinen und insofern möglicherweise Stoffwechselbesonderheiten der epidermalen Zelldifferenzierung reflektieren. Diese Hypothese wurde weiter untermauert, indem gezeigt werden konnte, daß lokale Applikation des Corticosteroids Triamcinolon in der Meerschweinchenepidermis selektiv tRNA serI und tRNA serV auf das Zwei- bis Dreifache erhöht. Die mögliche Bedeutung dieser Befunde wurde eingehend diskutiert.
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Formerly Assistant Professor at the Institute of Molecular Biology and Biochemistry, Freie Universität Berlin
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Schwarz, E., Gottschling, H. Changes in the serine-specific transfer ribonucleic acid pattern of guinea pig epidermis after corticosteroid treatment. Arch Dermatol Res 269, 153–159 (1980). https://doi.org/10.1007/BF00406535
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DOI: https://doi.org/10.1007/BF00406535