Summary
The cutaneous infiltrate in mycosis fungoides (MF) is predominantly composed of T4-positive T-cells. Attempts to distinguish the early stages of this condition from benign inflammatory infiltrates using anti-T3, T4 and other T-cell-associated antibodies have hitherto been unsucessful. Recently a monoclonal antibody BE 2 has been described as selectively reacting against leukemic cells in patients with cutaneous T-cell lymphoma. To investigate whether the BE 2 antigen is differentially expressed in different stages of MF and benign dermatoses, thus facilitating diagnosis, especially of early MF, the reactivity of monoclonal antibody BE 2 against cutaneous infiltrates from such conditions was assessed.
In the early stages of MF only a small number of reactive cells was present. In benign inflammatory infiltrates, especially in those that clinically and histologically were hardly distinguishable from early MF, BE 2 reactivity was essentially the same as in eczematous-stage MF. Lesions from plaque and tumor stage MF contained large numbers of BE 2-reactive cells. Our results indicate that expression of BE 2 is associated with the stage of a given MF lesion and is essentially identical in early MF and eczematous lesions with a similar histopathological appearance.
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Berger CL, Morrison S, Chu A, Patterson J, Estabrook A, Takezaki S, Sharon J, Wenburton D, Irigoyen O, Edelson RL (1983) Diagnosis of cutaneous T cell lymphoma by use of monoclonal antibodies reactive with tumor associated antigens. J Clin Invest 70:1203–1215
Brown JP, Woodbury RG, Hart CE, Hellström I, Hellström KE (1981) Quantitative analysis of melanoma associated antigen p 97 in normal and neoplastic tissues. Proc Natl Acad Sci USA 78:539–543
Burg G, Rodt H, Grosse-Wilde H, Braun-Falco O (1978) Surface markers and mitogen response of cells harvested from cutaneous infiltrates in mycosis fungoides and Sezary's syndrome. J Invest Dermatol 70:257–259
Cordell JL, Falini B, Erber WN, Gosh AK, Abdulaziz L, Mac Donald S, Pulford KAF, Stein H, Mason DY (1984) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229
Edelson RC, Smith RW, Frank MM, Green J (1973) Identification of subpopulations of mononuclear cells in cutaneous infiltrates. J Invest Dermatol 61:82–89
Edelson RC (1980) Cutaneous T cell lymphoma: Mycosis fungoides, Sezary's syndrome and other variants. J Am Acad Dermatol 2:89–106
Haynes BF, Metzgar RS, Minna JD, Burn RA (1980) Phenotypic characterization of cutaneous T cell lymphoma by use of monoclonal antibodies to compare with other malignant T cells. N Engl J Med 304:1319–1323
Holden CA, Morgan EW, MacDonald DM (1982) The cell population in the cutaneous infiltrate of mycosis fungoides: In situ studies using monoclonal antisera. Br J Dermatol 106:385–392
Kung PC, Berger CL, Goldstein G, LoGerfo P, Edelson RC (1984) Cutaneous T cell lymphoma. Characterisation by monoclonal antibodies. Blood 57:261–266
Lutzner M, Edelson R, Shein P (1975) Cutaneous T cell lymphomas. Am Intern Med 83:535–552
Rieber P, Lohmeyer J, Schendel DJ, Göttlinger H, Brodman S, Rank G, Heydecke S, Kopp E, Riethmüller G (1984) Characterisation of functional human T-cell subsets by monoclonal antibodies. In: Bernard A, Boumsel C, Dausse J, Milstein C, Schlossman SF (eds) Leukocyte typing. Springer, Berlin Heidelberg New York Tokyo, pp 303–311
Roberts J, Stead R, Rosenthal D, Tadros A, Jenkin P, Berger C, Edelson R, Sauder DN (1984) BE 1 and BE 2 monoclonal antibody reactivity in cutaneous T cell lymphoma. J Invest Dermatol 82:390 (abstract)
Thiers BH (1982) Controversies in mycosis fungoides. J Am Acad Dermatol 7:1–6
Willemze R, DeGraaff-Reitsma CM, Cnossen J, Van Vloten WA, Meijer CJLM (1983) Characterisation of T cell subpopulations in skin and peripheral blood of patients with cutaneous T cell lymphomas and benign inflammatory dermatoses. J Invest Dermatol 8:60–66
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Kaudewitz, P., Soldner, R., Burg, G. et al. Reactivity of monoclonal antibody BE 2 in different stages of mycosis fungoides and in benign dermal infiltrates. Arch Dermatol Res 279, 83–88 (1986). https://doi.org/10.1007/BF00417527
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DOI: https://doi.org/10.1007/BF00417527