Summary
We have examined the effects of several classes of metabolic inhibitors on the repair of potentially lethal damage in density-inhibited cultures of two rodent and two human cell systems which differ in their growth characteristics. Aphidicolin, 1-β-d-arabinofuranosylcytosine (ara-C) and hydroxyurea showed no effect on PLD repair, whereas the effects of 9-β-d-arabinofuranosyladenine (ara-A) and 3-aminobenzamide (3-AB) were cell line dependent. For example, 3-AB suppressed PLD repair almost completely in CHO cells, but showed no inhibitory effects in human diploid fibroblasts. These results indicate that inhibitors of DNA replication and poly(ADP-ribose) synthesis are not efficient inhibitors of cellular recovery in irradiated cells and, moreover, that such effects may be cell line dependent.
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Little, J.B., Ueno, A.M. & Dahlberg, W.K. Differential response of human and rodent cell lines to chemical inhibition of the repair of potentially lethal damage. Radiat Environ Biophys 28, 193–202 (1989). https://doi.org/10.1007/BF01211256
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DOI: https://doi.org/10.1007/BF01211256