Abstract
Vitamin B6 induces in vivo as well as in vitro the appearance of a puff at region 2–48C in Drosophila hydei. At concentrations of 10−2 M or lower, region 2–48C is the only region responding to vitamin B6 provided that oligomycin is present in the incubation medium. Pyridoxal phosphate and pyridoxamine phosphate supplied to medium containing oligomycin induce upon incubation of salivary glands a larger puff at 2–48C. — Puff 2–48C produces large quantities of a unique RNP-product; globular 140–220 Å particles which aggregate to stable complexes of 0.1–0.2 μ in diameter. Upon continuous in vitro incubation with vitamin B6, puff 2–48C becomes loaded with these aggregates which have never been observed in any other puff of Drosophila hydei.
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References
Ashburner, M.: Patterns of puffing activity in the salivary glands of Drosophila. V. Responses to environmental treatments. Chromosoma (Berl.) 31, 356–376 (1970).
Badaway, A. A. B.: The effects of salicylate on the activity of rat liver tyrosine-2-oxoglutarate aminotransferase in vitro and in vivo. Biochem. J. 126, 347–350 (1972).
Berendes, H. D.: Factors involved in the expression of gene activity in polytene chromosomes. Chromosoma (Berl.) 24, 418–437 (1968a).
Berendes, H. D.: Electron microscopical mapping of giant chromosomes. Dros. Inf. Serv. 43, 115–116 (1968b).
Berendes, H. D.: The control of puffing in Drosophila hydei. In: Developmental studies on giant chromosomes (W. Beermann, ed.), p. 181–207. Berlin-Heidelberg-New York: Springer 1972.
Berendes, H. D., Breugel, P. M. A. van, Holt, Th. K. H.: Experimental puffs in Drosophila hydei salivary gland chromosomes. Chromosoma (Berl.) 16, 35–47 (1965).
Berendes, H. D., Meyer, G. F.: A specific chromosome element, the telomere of Drosophila polytene chromosomes. Chromosoma (Berl.) 25, 184–197 (1968).
Boyd, J. B., Berendes, H. D., Boyd, H.: Mass preparation of nuclei from the larval salivary glands of Drosophila hydei. J. Cell Biol. 38, 369–378 (1968).
Georgiev, G. P., Samarina, O. P.: D-RNA containing ribonucleoprotein particles. Advanc. Cell Biol. 2, 47–110 (1971).
Leenders, H. J., Beckers, P. J. A.: The effect of changes in the respiratory metabolism upon genome activity. A correlation between induced gene activity and an increase in activity of a respiratory enzyme. J. Cell Biol. 55, 257–265 (1972).
Leenders, H. J., Berendes, H. D.: The effect of changes in the respiratory metabolism upon genome activity in Drosophila. I. The induction of gene activity. Chromosoma (Berl.) 37, 433–444 (1972).
Leenders, H. J., Wullems, G. J., Berendes, H. D.: Competitive interaction of adenosine 3′5′-monophosphate on gene activation by ecdysterone. Exp. Cell Res. 63, 159–164 (1970).
Meister, A.: Biochemistry of amino acids, vol. 1. New York-London: Acad. Press 1965.
Swift, H.: Cytochemical studies on nuclear fine structure. Exp. Cell Res., Suppl. 9, 54–67 (1963).
Swift, H.: Molecular morphology of the chromosome. In: The chromosome. In Vitro 1, 26–49 (1985).
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Leenders, H.J., Derksen, J., Maas, P.M.J.M. et al. Selective induction of a giant puff in Drosophila hydei by vitamin B6 and derivatives. Chromosoma 41, 447–460 (1973). https://doi.org/10.1007/BF00396502
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DOI: https://doi.org/10.1007/BF00396502