Abstract
Incubation of Chironomus pallidivittatus salivary glands in the presence of cordycepin induces the collapse of Balbiani rings. In spite of the great reduction of the Balbiani ring size, 3H-uridine incorporation takes place in these structures and also in puffs and nucleolus. Modifications in the nucleolar pattern of labelling have been observed. Differences were found between the effect of cordycepin and those of actinomycin and adenosine both of which induce the collapse of Balbiani rings.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beermann, W.: Chromomerenkonstanz und spezifische Modifikationen der Chromosomenstruktur in der Entwicklung und Organdifferenzierung von Chironomus tentans. Chromosoma (Berl.) 5, 139–198 (1952).
Beermann, W.: Differentiation at the level of the chromosomes. In: Cell differentiation and morphogenesis, p. 24–54. Amsterdam: North-Holland Publ. Co. 1966.
Beermann, W.: Effect of α-amanitine on puffing and intranuclear RNA synthesis in Chironomus salivary glands. Chromosoma (Berl.) 34, 152–167 (1971).
Darnell, J. E., Philipson, L., Wall, R., Adesnik, M.: Polyadenylic acid sequences: Role in conversion of nuclear RNA into messenger RNA. Science 174, 507–510 (1971).
Giménez-Martín, G., De la Torre, C., Fernández-Gómez, M. E., González-Fernández, A.: Effect of the cordycepin on the nucleolar cycle. Caryologia (Firenze) 25, 43–58 (1971).
Hastings, J. R. B., Kirby, K. S.: The nucleic acids of Drosophila melanogaster. Biochem. J. 100, 532–539 (1966).
Loening, U. E.: The fractionation of high-weight ribonucleic acid by polyacryl amide-gel electrophoresis. Biochem. J. 102, 251–257 (1967).
Pelling, C., Beermann, W.: Diversity and variation of the nucleolar organizing regions in chironomids. Nat. Cancer. Inst. Monogr. 23, 393–409 (1965).
Penman, S., Fan, H., Perlman, S., Rosbash, M., Weinberg, R., Zylber, E.: Distinct RNA synthesis systems of the HeLa cell. Cold Spr. Harb. Symp. quant. Biol. 35, 561–575 (1970).
Philipson, L., Wall, R., Glickman, G., Darnell, J. E.: Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication. Proc. nat. Acad. Sci. (Wash.) 68, 2806–2809 (1971).
Rosbash, M., Penman, S.: Messenger and heterogeneous nuclear RNA in HeLa cells: Differential inhibition by cordycepin. Proc. nat. Acad. Sci. (Wash.) 67, 1878–1885 (1970).
Rubinstein, L., Clever, U.: Non-conservative processing of ribosomal RNA in an insect, Chironomus tentans. Biochim. biophys. Acta (Amst.) 246, 517–529 (1971).
Siev, M., Weinberg, R., Penman, S.: The selective interruption of nucleolar RNA synthesis in HeLa cells by cordycepin. J. Cell Biol. 41, 510–520 (1969).
Stockert, J. C., Fernandez-Gomez, M. E., Sogo, J. M., Lopez-Saez, J. P.: Nucleolar segregation by adenosine 3′-deoxyriboside (cordycepin) in root-tip cells of Allium cepa. Exp. Cell Res. 59, 85–89 (1970).
Wobus, U., Panitz, R., Serfling, E.: α-amanitine: Its effect on RNA synthesis in polytene chromosomes. Experientia (Basel) 27, 1202–1203 (1971).
Wolstenholme, D. R.: The distribution of DNA and RNA in salivary glands chromosomes of Chironomus tentans as revealed by fluorescence microscopy. Chromosoma (Berl.) 17, 219–229 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Diez, J.L. Effect of cordycepin (3′-desoxyadenosine) on polytene chromosomes of Chironomus pallidivittatus salivary glands. Chromosoma 42, 345–358 (1973). https://doi.org/10.1007/BF00399404
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00399404