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Complexes de transcription d'origines nucléolaire et chromosomique d'ovocytes de Pleurodeles waltlii et P. poireti (Amphibiens, Urodèles)

Transcription complexes with nucleolar and chromosomal origins in oocytes of Pleurodeles waltlii and P. poireti (Amphibia, Urodela)

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Abstract

The method of spreading transcription complexes has been applied to amphibian oocytes of Pleurodeles genus. Complexes of nucleolar origin show a regular and homogeneous organization similar to that described in other materials. The observations add to the interpretation as an amplification of nucleolar DNA and a redundancy of ribosomal cistrons in the two species studied. —On the other hand, complexes of chromosomal origin display a great diversity. Two main characteristics can be drawn: the existence of several transcription units in a chromosomal organization unit and the possibility to point out a special architecture at the RNP fibril level. Applying a shadowing technique used for isolated molecules is an improvement compared with earlier methods based on PTA coloration.

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Authors and Affiliations

  1. Centre de Recherches d'Ivry, Université Pierre et Marie Curie, 67, rue Maurice, Gunsbourg, 94200, Ivry., France

    N. Angelier & J. C. Lacroix

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  1. N. Angelier
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  2. J. C. Lacroix
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Attachée de recherches C.N.R.S, Laboratoire de Biologie Animale, Orléans.

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Angelier, N., Lacroix, J.C. Complexes de transcription d'origines nucléolaire et chromosomique d'ovocytes de Pleurodeles waltlii et P. poireti (Amphibiens, Urodèles). Chromosoma 51, 323–335 (1975). https://doi.org/10.1007/BF00326319

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  • DOI: https://doi.org/10.1007/BF00326319

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