Abstract
The ultrastructural features of chromosome vesicle formation in early sea urchin embryos and chromosome vesicle uptake of tritiated thymidine is described. Envelopes which resemble typical nuclear envelopes form around the condensed anaphase chromosomes. In late anaphase or early telophase, the chromosomes swell and decondense and it is at this time when tritiated thymidine is incorporated. This study shows that DNA synthesis in the rapidly dividing cells of early sea urchin embryos occurs in chromosome vesicles which form during anaphase.
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References
Böhm, N.: In: Techniques of biochemical and biophysical morphology (D. Glick and R.M. Rosenbaum, eds.), pp. 89–141. New York: Wiley-Interscience 1972
Boveri, Th.: Zellenstudien IV. Über die Natur der Zentrosomen. Jena. Z. Naturwiss. 35, 1–220 (1901)
Conklin, E.G.: The individuality of the germ nuclei during the cleavage of the egg of Crepidula. Biol. Bull. 2, 257–265 (1901)
Dan, K., Tanaka, S., Yamazaki, K., and Kato, Y.: Cell cycle study up to the time of hatching in the embryo of the sea urchin. Develop., Growth and Differ. 22, 589–598 (1980)
Emerson, C.P., Humphreys, T.: Ribosomal RNA synthesis and the multiple atypical nucleoli in cleaving embryos. Science 171, 899–901 (1971)
Goodenough, D.A., Ito, S., and Revel, J.P.: Electron microscopy of early cleavage stages in Arbacia punctulata. Biol. Bull. 135, 420–421 (abstract) (1968)
Harris, P.: Mitosis in sea urchin blastomeres. J. Cell Biol. 11, 419–432 (1961)
Harvey, E.B.: The American Arbacia and other sea urchins, p. 97. Princeton, N.J.: Princeton University Press 1956
Hay, E.D. In: The Nucleus (F. Haguenau and A. Dalton, eds.), pp. 1–79. New York: Academic Press 1968
Ito S., Karnovsky, M.J.: Formaldehyde-glutaraldehyde fixatives containing trinitrocompounds. J. Cell Biol. 39, 168a (Abstract) (1968)
Longo, F.J.: An ultrastructural analysis of mitosis and cytokinesis in the zygote of the sea urchin, Arbacia punctulata. J. Morph. 138, 207–215 (1972)
Merriam, R.W.: Nuclear envelope structure during cell division in Chaetopterus eggs. Exp. Cell Res. 22, 93–107 (1961)
Richards, A.: The history of the chromosome vesicles in Fundulus and the theory of genetic continuity of chromosomes. Biol. Bull. 32, 249–290 (1917)
Surrey, S.: Ribosomal RNA synthesis in pre- and post-gastrula-stage sea urchin embryos. Develop. Biol. 71, 83–99 (1979)
Wilson, E.B.: The Cell in Development and Inheritance, 2nd ed., p. 107. London: Macmillan Co. 1900
Wilson, E.B.: The Cell in Development and Heredity, 3rd ed. (pp. 221, 439, 760, and 894). New York: Macmillan Co. 1925
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Ito, S., Dan, K. & Goodenough, D. Ultrastructure and 3H-thymidine incorporation by chromosome vesicles in sea urchin embryos. Chromosoma 83, 441–453 (1981). https://doi.org/10.1007/BF00328271
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DOI: https://doi.org/10.1007/BF00328271