Summary
A reliable and sensitive method has been developed to assess the concentrations of the hypnotic drug triazolam in human tissues, including putrefied tissues. The method involves a 3-step solvent extraction, cleanup on a silica gel column and gas chromatography using a nitrogen phosphorus detector and a capillary column. Estazolam was used as an internal standard. The calibration curve was linear over the concentration range 1 ng/g1 gm/g and the lower limit of detection was 0.5 ng/g. A forensic study was performed on the toxicological effects of triazolam using putrefied tissues.
Zusammenfassung
Eine zuverlässige und empfindliche Methode wurde entwickelt, um die Konzentrationen des hypnotischen Medikaments Triazolam in menschlichen Geweben, einschließlich fauler Gewebe bestimmen zu können. Die Methode besteht aus einer dreistufigen Flüssig-Flüssig-Extraktion, einem Clean up an Kieselgel und Gaschromatographie mit einem Stickstoff-PhosphorDetektor und einer Kapillarsäule. Als interner Standard wurde Estazolam benutzt. Die Eichkurve war im Konzentrationsbereich zwischen 1 ng/g und 1 gg/g linear, und die untere Nachweisgrenze betrug 0.5 ng/g. Es wurde eine forensische Untersuchung über die toxikologischen Effekte von Triazolam an faulem Gewebe durchgeführt.
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Guest researcher from Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, Fukuoka, Japan
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Kudo, K., Nagata, T., Imamura, T. et al. Forensic analysis of triazolam in human tissues using capillary gas chromatography. Int J Leg Med 104, 67–69 (1991). https://doi.org/10.1007/BF01626033
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DOI: https://doi.org/10.1007/BF01626033