Summary
A novel mouse monoclonal antibody (P4-5C) has been developed which recognizes the core portion of the protein carrying ABO(H) blood group antigens in human saliva. This proved to be specific for human saliva using immunochemical investigations such as enzymelinked immunosorbent assay, Ouchterlony method and counter-immunoelectrophoresis. By licht and electron microscope studies with immunohistochemical techniques using this human saliva-Specific P4-5C as primary antibody, it was shown that P4-5C reacted specifically and exclusively with mucus from the mucous gland cells of human salivary glands. P4-5C reacted neither with the mucous gland cells of other primates (hamadryas baboon, Japanese monkey and Rhesus monkey) and four mammals (dog, cat, rabbit and mouse) nor with other human tissues. The epitope on the core portion of the ABO(H)-carrying protein was defined by P4-5C and could be discriminated from the epitope of ABO(H) blood group antigens using immunoelectronmicroscopy, although these 2 epitopes were localized relatively close to each other. The P4-5C monoclonal antibody can be also used for morphological species identification of tissue specimens from submandibular glands.
Zusammenfassung
Ein neuartiger monoklonaler Antikörper von der Maus (P4-5C) wurde entwickelt, der den Kern des Proteins erkennt, welches die ABO-Blutgruppenantigene im menschlichen Speichel trägt. Dieser erwies sich als spezifisch für menschlichen Speichel über die Benutzung immunchemischer Methoden, wie enzymgekoppelter Immunabsorptions-Test, OuchterlonyMethode, Kreuz-Elektrophorese. Durch licht- und elektronenmikroskopische Untersuchungen mit immunhistochemischen Techniken unter Verwendung dieses Antikörpers konnte gezeigt werden, daß eher spezifisch und exklusiv mit Schleim von den mukösen Zellen der menschlichen Speicheldrüsen reagiert. P4-5C reagierte weder mit mukösen Zellen anderer Primaten (Mantelpavian, japanischer Affe, Rhesus-Affe) und 4 Säugetieren (Hund, Katze, Ratte und Maus) noch mit anderen menschlichen Geweben. Das Epitop des Kernanteils des ABH-tragenden Proteins wurde definiert als P4-5C und konnte von dem Epitop der ABO-Blutgruppen-Antigene unterschieden werden mit Hilfe der Immun-Elektronenmikroskopie, obwohl diese beiden Epitope relativ nahe beieinander lokalisiert waren. Der P4-5C monoklonale Antikörper kann auch benutzt werden für die morphologische Spezies-Identifikation von Gewebsproben von den Glandulae submandibularis.
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Ohshima, T., Nagano, T., Kimura, A. et al. Immunohistochemical study on the localization of the epitope defined by a human saliva-specific mouse monoclonal antibody (P4-5C). Int J Leg Med 104, 137–140 (1991). https://doi.org/10.1007/BF01369717
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DOI: https://doi.org/10.1007/BF01369717
Key words
- Immunohistochemistry
- Salivary gland
- Specific monoclonal antibody
- ABO(H) blood group antigens
- Species identification