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Leukocyte adhesion molecules in rejected corneal allografts

  • Clinical investigation
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Abstract

Leukocyte adhesion molecules are believed to play a key role in the selective recruitment of different leukocyte populations to inflammatory sites. In this study, we investigated the presence and distribution of intercellular ad hesion molecule-1 (ICAM-1), E-selectin (endothelial leukocyte adhesion molecule-1) and vascular cell adhesion molecule-1 (VCAM1) in 12 rejected corneal allografts and compared the presence of these adhesion molecules with the composition of the associated inflammatory infiltrates. ICAM-1 was focally expressed on corneal epithelial cells and its expression was increased on keratocytes, corneal and vascular endothelial cells particulary at the site of dense infiltration with mononuclear leukocytes. E-selectin was present on endothelial cells of vessels in the stroma of rejected corneal allografts which were characterized by dense infiltration with T cells and macrophages. VCAM-1 was predominantly expressed on inflammatory cells of the macrophage/monocyte lineage, but only sporadically on vascular endothelial cells in thet stroma of vascularized rejected corneal allografts. Our results suggest that ICAM-1, E-selectin and VCAM-1 may all be involved in the pathogenesis of corneal allograft rejection, particulary in the generation of the inflammatory infiltrates.

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References

  1. Abu El-Asrar AM, Geboes K, Missotten L, Emarah MH, Desmet V (1990) Expression of MHC class 11 antigens and immunoglobulin M by the corneal epithelial cells in herpetic keratitis. Intern Ophthalmol 14:233–239.

    Google Scholar 

  2. Becker-André M, van Huijsduijnen RH, Losberger C, Whelan J, Delamarter JF (1992) Murine endothelial leukocyte adhesion molecule-1 is a close structural and functional homologue of the human protein. Eur J Biochem 206:401–411.

    Google Scholar 

  3. Bevilacqua MP, Stengelin S, Gimbrone Jr MA, Seed B (1989) Endothelial leukocyte adhesion molecule-1: an inducible receptor for neurotrophils related to complement regulatory proteins and lectins. Science 243:1160–1165.

    Google Scholar 

  4. Bevilacqua MP, Butcher E, Furie B, Furie B, Gallatin M, Gimbrone M, Harlan J, Kishimoto K, Lasky L, McEver R, Paulson J, Rosen S, Seed B, Siegelman M, Springer T, Stoolman L, Tedder T, Varki A, Wagner D, Weissman I, Zimmerman G (1991) Selectins: a family of adhesion receptors. Cell 67:233.

    Google Scholar 

  5. Bonnard C, Papermaster DS, Kraehenbuhl JP (1984) The streptavidin-biotin bridge technique: application in light and electron microscope immunocytochemistry. In: Polak JM, Varndell IM (eds) Immunolabelling for electron microscopy. Elsevier, Amsterdam, pp 95–111.

    Google Scholar 

  6. Briscoe DM, Schoen FJ, Rice GE, Bevilacqua MP, Ganz P, Pober JS (1991) Induced expression of endothelial-leukocyte adhesion molecules in human cardiac allografts. Transplantation 51:537–547.

    Google Scholar 

  7. Callanan D, Peeler J, Niederkorn JY (1988) Characteristics of rejection of orthotopic corneal allografts in the rat. Transplantation 45:437.

    Google Scholar 

  8. Carlos TM, Schwartz BR, Kovach NL, Yee E, Rosso M, Osborn L, ChiRosso G, Newman B, Lobb R, Harlan JM (1990) Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial cells. Blood 76:965–970.

    Google Scholar 

  9. Damle NK, Doyle LV (1990) Ability of human T lymphocytes to adhere to vascular endothelial cells and to augment endothelial permeability to macromolecules is linked to their state of post-thymic maturation. J Immunol 144:1233.

    Google Scholar 

  10. De Maeyer E, De Maeyer-Guignard J (1988) Interferons and other regulatory cytokines. Wiley, New York.

    Google Scholar 

  11. De-Panfilis G, Manara GC, Ferrari C, Torresani C (1990) Adhesion molecules on the plasma membrane of epidermal cells. 11. The intercellular adhesion molecule-1 is constitutively present on the cell surface of human resting Langerhans cells. J Invest Dermatol 94:317–321.

    Google Scholar 

  12. Dougherty GJ, Murdoch S, Hogg N (1988) The function of human intercellular adhesion molecule-1 (ICAM1) in the generation of an immune response. Eur J Immunol 18:35–39.

    Google Scholar 

  13. Dreizen NG, Whitsett CF, Stulting RD (1988) Modulation of HLA-DR antigen expression on corneal epithelial and stromal cells. Invest Ophthalmol Vis Sci 29:933–939.

    Google Scholar 

  14. Dustin ML, Rothlein R, Bhan AK, Dinarello CA, Springer TA (1986) Induction by IL-1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J Immunol 137:245–254.

    Google Scholar 

  15. Elices MJ, Osborn L, Takada Y, Crouse C, Luhowskyj S, Hemler ME, Lobb RR (1990) VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA 4/fibronectin binding site. Cell 60:577–584.

    Google Scholar 

  16. Elner VM, Elner SG, Pavilack MA, Todd RF, Yue BYJT, Huber AR (1991) Intercellular adhesion molecule-1 in human corneal endothelium: modulation and function. Am J Pathol 138:525–536.

    Google Scholar 

  17. Elner VM, Dutt S, Pavilack MA, Sugar A, Foster CS, Elner SG (1992) Intercellular adhesion molecule-1 (ICAM-1) and HLA-DR antigens in herpes keratitis. Ophthalmology 99:1400–1407.

    CAS  PubMed  Google Scholar 

  18. Griffiths CEM, Voorhees JJ, Nickoloff BJ (1989a) Characterization of intercellular adhesion molecule-1 and HLA-DR expression in normal and inflamed skin: modulation by recombinant gamma interferon and tumor necrosis factor. J Am Acad Dermatol 20:617–629.

    Google Scholar 

  19. Griffiths CEM, Voorhees JJ, Nickoloff BJ (1989b) Gamma interferon induces different keratinocyte cellular patterns of expression of HLA-DR and DQ and intercellular adhesion molecule-1 (ICAM-1) antigens. Br J Dermatol 120:1–7.

    Google Scholar 

  20. Heukart P, Yue CC (1988) The role of cytoplasmic granules in lymphocyte cytotoxicity. Prog Allergy 40:82.

    Google Scholar 

  21. Koch AE, Burrows JC, Haines GK, Carlos TM, Harlan JM, Leibovich SJ (1991) Immunolocalization of endothelial and leukocyte adhesion molecules in human rheumatoid and osteoarthritic synovial tissues. Lab Invest 64:313–320.

    Google Scholar 

  22. Lindstrom RL (1986) Advances in corneal transplantation. N Eng] J Med 315:1.

    Google Scholar 

  23. Lisby S, Ralfkiaer E, Rothlein R, Vejlsgaard GL (1989) Intercellular adhesion molecule-1 (ICAM-1) expression correlated to inflammation. Br J Dermatol 120:479–484.

    Google Scholar 

  24. Lobb RR, Chi-Rosso G, Leone DR, Rosa MD, Bixler S, Newman BM, Luhowskyj S, Benjamin CD, Dougas IG, Goelz SE, Hession C, Pingchang Chow E (1991) Expression and functional characterization of a soluble form of endothelial-leukocyte adhesion molecule 1. J Immunol 147:124–129.

    Google Scholar 

  25. Makgoba MW, Sanders ME, Ginther Luce GE, Gugel EA, Dustin ML, Springer TA, Shaw S (1988) Functional evidence that intercellular adhesion molecule-1 (ICAM-1) is a ligand for LFA-1-dependent adhesion in T cell-mediated cytotoxicity. Eur J Immunol 18:637–640.

    Google Scholar 

  26. Makgoba MW, Sanders ME, Shaw S (1989) The CD2-LFA3 and LFA-1ICAM pathways: relevance to T cell recognition. Immunol Today 10:417.

    Google Scholar 

  27. Marlin SD, Springer TA (1987) Purified intercellular adhesion molecule-1 is a ligand for lymphocyte function-associated antigen 1. Cell 51:813.

    Article  CAS  PubMed  Google Scholar 

  28. McBride BW, McGill JI, Smith JL (1988) MHC class I and class II antigen expression in normal human corneas and in corneas from cases of herpetic keratitis. Immunology 65:583–587.

    Google Scholar 

  29. Mukai K, Rosai J, Burgdorf WHC (1980) Localization of factor VIII-related antigen in vascular endothelial cells using an immunoperoxidase method. Am J Surg Pathol 4:273–276.

    Google Scholar 

  30. Munro JM, Pober JS, Cotran RS (1989) Tumor necrosis factor and interferon-γ induce distinct patterns of endothelial activation and associated leukocyte accumulation in skin of Papio anubis. Am J Pathol 135:121.

    Google Scholar 

  31. Nickoloff BJ, Griffiths CEM (1989) T lymphocytes and monocytes bind to keratinocytes in frozen sections of biopsy specimens of normal skin treated with gamma interferon. J Am Acad Dermatol 20:736–743.

    Google Scholar 

  32. Pavilack MA, Elner VM, Elner SG, Todd RF, Huber AR (1992) Differential expression of human corneal and perilimbal ICAM-1 inflammatory cytokines. Invest Ophthalmol Vis Sci 33:564–573.

    Google Scholar 

  33. Peeler J, Niederkorn JY, Matoba A (1985) Corneal allografts induce cytotoxic T cell but not delayed hypersensitivity responses in mice. Invest Ophthalmol Vis Sci 26:1516.

    Google Scholar 

  34. Pepose JS, Gardner KM, Nestor MS, Foos RY, Pettit TH (1985a) Detection of HLA class I and Il antigens in rejected human corneal allografts. Ophthalmology 92:1480–1484.

    Google Scholar 

  35. Pepose JS, Nestor MS, Gardner KM, Foos RY, Pettit TH (1985b) Composition of cellular infiltrates in rejected human corneal allografts. Graefe's Arch Clin Exp Ophthalmol 222:128–133.

    Google Scholar 

  36. Pober JS, Cotran RS (1990) The role of endothelial cells in inflammation. Transplantation 50:537–544.

    Google Scholar 

  37. Pober JS, Cotran RS. Editorial (1991) What can be learned from the expression of endothelial adhesion molecules in tissues. Lab Invest 64:301–305.

    Google Scholar 

  38. Rice GE, Munro JM, Corless C, Bevilacqua MP (1991) Vascular and non-vascular expression of INCAM-110. A target for mononuclear leukocyte adhesion in normal and inflamed human tissues. Am J Pathol 138:385–393.

    Google Scholar 

  39. Rothlein R, Dustin ML, Marlin SD, Springer TA (1986) A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J Immunol 137:1270–1274.

    Google Scholar 

  40. Rothlein R, Czajkowski M, O'Neill MM, Marlin SD, Mainolfi E, Merluzzi VJ (1988) Induction of intercellular adhesion molecule-1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies. J Immunol 141:1665–1669.

    Google Scholar 

  41. Schwinzer R (1989) Cluster report: CD 45/CD 45R. In: Knapp W et al. (eds) Leukocyte typing IV. White cell differentiation antigens. Oxford University Press, Oxford, pp 628–634.

    Google Scholar 

  42. Shimizu Y, Shaw S, Graber N, Gopal TV, Horgan KJ, Van Seventer GA, Newman W (1991) Activation-independent binding of human memory T cells to adhesion molecule SLAM-1. Nature 349:799–802.

    Google Scholar 

  43. Springer TA (1990) Adhesion receptors of the immune system. Nature 346:425–434.

    Google Scholar 

  44. Vantrappen L, Geboes K, Missotten L, Maudgal PC, Desmet V (1985) Lymphocytes and Langerhans cells in the normal human cornea. Invest Ophthalmol Vis Sci 26:220–225.

    Google Scholar 

  45. Vejlsgaard GL, Ralfkiaer E, Avnstorp C, Czajkowski M, Marlin ST, Rothlein R (1989) Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin lesions and malignant cutaneous lymphomas. J AM Acad Dermatol 20:782–790.

    Google Scholar 

  46. Walsh LJ, Lavker RM, Murphy GF (1990) Biology of disease. Determinants of immune cell trafficking in the skin. Lab Invest 63:592–600.

    Google Scholar 

  47. Young E, Stark WJ, Prendergast RA (1985) Immunology of corneal allograft rejection: HLA-DR antigens on human corneal cells. Invest Ophthalmol Vis Sci 26:571–574.

    Google Scholar 

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  1. Abteilung für Ophthalmologie, Universität Innsbruck, Anichstrasse 35, A-6020, Innsbruck, Austria

    Wolfgang Philipp

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Philipp, W. Leukocyte adhesion molecules in rejected corneal allografts. Graefe's Arch Clin Exp Ophthalmol 232, 87–95 (1994). https://doi.org/10.1007/BF00171669

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  • DOI: https://doi.org/10.1007/BF00171669

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