Skip to main content
SpringerLink
Log in

Zur Histochemie von Hydrolasen im Hoden des Hundes und der Katze

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

The localization of unspecific esterase, alkaline phosphatase, adenosine triphosphatase, 5′-nucleotidase, and β-D-glucuronidase in the testes of cat and dog was demonstrated by histochemical means. We observed a strong esterase activity in the Sertoli cells of the cat and high amounts of alkaline phosphatase and adenosine triphosphatase in the membrana propria of all seminiferous tubules. In both species the principal site of β-D-glucuronidase was in the Leydig cells. Our findings obtained being discussed.

Zusammenfassung

Wir untersuchten das Verteilungsmuster von unspezifischer Esterase, alkalischer Phosphatase, Adenosintriphosphatase, 5′-Nucleotidase und β-D-Glucuronidase im Hoden von Hund und Katze. Besonders hervorzuheben sind eine starke Aktivität der unspezifischen Esterase in den Sertolizellen der Katze, der Reichtum der Membrana propria aller Hodentubuli an alkalischer Phosphatase und Adenosintriphosphatase sowie die kräftige Reaktion auf β-D-Glucuronidase in den Leydigzellen beider Tierarten.

Die Befunde werden diskutiert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Baust, P., H. G. Goslar u. E. Tonutti: Das Verhalten der Esterasen im Hoden von Ratte, Maus und Meerschweinchen nach Östrogenbehandlung. Z. Zellforsch. 69, 686–698 (1966).

    Google Scholar 

  • Billet, F., and S. M. McGee-Russel: The histochemical localization of β-glucuronidase in the liver of the newt. Quart. J. micr. Sci. 97, 155–159 (1956).

    Google Scholar 

  • Braun-Falco, O.: Zur Histotopographie der β-Glucuronidase in normaler menschlicher Haut. Arch. klin. exp. Derm. 203, 61–67 (1956).

    Google Scholar 

  • Campbell, J. G.: The intracellular localization of β-glucuronidase. Brit. J. exp. Path. 30, 548–554 (1949).

    Google Scholar 

  • Chauncey, H. H., and G. Quintarelli: Localization of acid phosphatase, nonspecific esterase and β-D-galactosidase in parotid and submaxillary glands of domestic and laboratory animals. Amer. J. Anat. 108, 263–293 (1961).

    Google Scholar 

  • Deimling, O. H. v.: Die Darstellung phosphatfreisetzender Enzyme mittels Schwermetall-Simultan-Methode. Histochemie 4, 48–55 (1964).

    Google Scholar 

  • Fishman, W. H.: β-Glucuronidase. In: Advances in enzymology (ed. by F. F. Nord), vol. 26. New York and London: Interscience Publ. 1955.

    Google Scholar 

  • —: Rat skeleton muscle β-glucuronidase localization by postcoupling technique using naphthol AS-BI, β-D-glucosiduronic acid and fast blue R. J. Histochem. Cytochem. 4, 306–308 (1956).

    Google Scholar 

  • —, and A. J. Anlyan: β-glucuronidase activity in human tissues. Cancer Res. 7, 808–817 (1947).

    Google Scholar 

  • —, and J. R. Baker: Cellular localization of β-glucuronidase in rat tissues. J. Histochem. Cytochem. 4, 570–587 (1956).

    Google Scholar 

  • —, and L. W. Fishman: The elevation of uterine β-glucuronidase activity by estrogenic hormones. J. biol. Chem. 152, 487–488 (1944).

    Google Scholar 

  • —, St. S. Goldman, and S. Green: Several biochemical criteria for evaluating β-glucuronidase localization. J. Histochem. Cytochem. 4, 239–251 (1956).

    Google Scholar 

  • Gomori, G.: Distribution of lipase in the tissues under normal and pathologic conditions. Arch. Path. 41, 121–129 (1946).

    Google Scholar 

  • —: Histochemical specifity of phosphatases, Proc. Soc. exp. Biol. (N.Y.) 70, 7–11 (1949).

    Google Scholar 

  • Hayashi, M., J. Nakajima, and W. H. Fishman: The cytologie demonstration of β-glucuronidase employing naphthol AS-BI glucuronide and hexazonium pararosanilin; a preliminary report. J. Histochem. Cytochem. 4, 293–297 (1956).

    Google Scholar 

  • —, K. Ogata, T. Shiraogawa, Z. Murakami, and S. Tuneishi: The effect of unilateral and bilateral cryptorchidisms upon the activities of β-glucuronidase, lipase and esterase in the rat testis. Acta path. jap. 8, 113–126 (1958).

    Google Scholar 

  • —, K. Simoda, K. Ogata, T. Takamori, T. Shiraogawa, and O. Kawase: Histochemical studies on the uterine β-glucuronidase, sulfatage and esterase activities during the rat estrous cycle. Acta histochem. (Jena) 3, 277–283 (1956).

    Google Scholar 

  • Hosoyama, Y., and T. Shimadate: Alkaline phosphatase of rat testis. Zool. Mag. 75, 71–76 (1966).

    Google Scholar 

  • Huggins, Ch., and St. H. Moulton: Esterases of testis and other tissues. J. exp. Med. 88, 169–179 (1948).

    Google Scholar 

  • Kagawa, S., and K. Osanai: Histochemical demonstration of hydrolytic enzymes and dehydrogenases in the oral mucosa of cats. J. Histochem. Cytochem. 11, 635–640 (1963).

    Google Scholar 

  • Kawakatsu, K., M. Mori, K. Fujita, M. Fukuda, and S. Deguchi: Histochemical studies on salivary glands. VI. Histochemical demonstration of β-glucuronidase, β-galactosidase and β-glucosidase in normal salivary gland. Arch. Histol. jap. 19, 533–546 (1960).

    Google Scholar 

  • —, M. Fukuda, S. Kagawa, and K. Osanai: Histochemical studies of the developing submaxillary gland in the human fetus. J. Osaka Univ. dent. Soc. 3, 3–9 (1963).

    Google Scholar 

  • Kühnel, W., u. K.-H. Wrobel: über die histochemisch faßbare Aktivität der β-D-Glucuronidase und der β-D-Galaktosidase in der Harderschen Drüse des Kaninchens. Albrecht v. Graefes Arch. Ophthal. 171, 173–183 (1966).

    Google Scholar 

  • Leonhard, S. L., and E. Knobil: β-Glucuronidase activity in the rat uterus. Endocrinology 47, 331–337 (1950).

    Google Scholar 

  • Mancini, R. E., J. Nolazco, and F. A. de la Balze: Histochemical study of normal adult human testis. Anat. Rec. 114, 127–147 (1952).

    Google Scholar 

  • Montagna, W.: Histology and cytochemistry of human skin. XI. The distribution of β-glucuronidase. J. biophys. biochem. Cytol. 3, 343–348 (1957).

    Google Scholar 

  • Nachlas, M. M., D. T. Crawford, and A. M. Seligman: The histochemical demonstration of leucine aminopeptidase. J. Histochem. Cytochem. 5, 264–278 (1957).

    Google Scholar 

  • —, and A. M. Seligman: The comparative distribution of esterase in the tissues of five mammals by a histochemical technique. Anat. Rec. 105, 677–696 (1949).

    Google Scholar 

  • Niemi, M., M. Härkönen, and A. Kokko: Localization and identification of testicular esterases in the rat. J. Histochem. Cytochem. 10, 186–193 (1962).

    Google Scholar 

  • Odell, O. D., and W. H. Fishman: Studies on β-Glucuronidase. I. Activities in human endometrium. Amer. J. Obstet. Gynec. 59, 200–203 (1950).

    Google Scholar 

  • Ogata, T., and M. Mori: A histochemical study of hydrolytic enzymes in muscle fibers of various animals. J. Histochem. Cytochem. 11, 645–652 (1963).

    Google Scholar 

  • Schmidt, F. C.: Licht-und elektronenmikroskopische Untersuchungen am menschlichen Hoden und Nebenhoden. Z. Zellforsch. 63, 707–727 (1964).

    Google Scholar 

  • Seligman, A. M., K.-Ch. Tsou, S. H. Rutenburg, and R. B. Cohen: Histochemical demonstration of β-Glucuronidase with a synthetic substrate. J. Histochem. Cytochem. 2, 209–229 (1954).

    Google Scholar 

  • Shantaveerappa, T. R., and G. H. Bourne: Histochemical studies on the distribution of β-glucuronidase in the eye. Histochemie 3, 413–421 (1964).

    Google Scholar 

  • Steigleder, G. K.: Über den Nachweis der β-Glucuronidase auf der Hautoberfläche. Klin. Wschr. 36, 984–985 (1958).

    Google Scholar 

  • Verne, J., et S. Hébert: L'activité esterasique des cellules interstitielles du testicule et de l'épididyme chez le rat. Ann. Endocr. (Paris) 13, 924–930 (1952).

    Google Scholar 

  • Wrobel, K.-H., u. W. Kühnel: Die Histotopik einiger Oxydoreductasen im Hoden von Hund und Katze. Histochemie 10, 208–215 (1967).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Anatomisches Institut der Universität Marburg a.d. Lahn, Deutschland

    Karl -Heinz Wrobel & Wolfgang Kühnel

Authors
  1. Karl -Heinz Wrobel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wolfgang Kühnel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wrobel, K.H., Kühnel, W. Zur Histochemie von Hydrolasen im Hoden des Hundes und der Katze. Histochemie 10, 329–335 (1967). https://doi.org/10.1007/BF00304315

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00304315

Search

Navigation