Summary
In adrenalectomized rats, histochemical and immunohistochemical properties of the following secretion products have been investigated:
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1.
CRF-granules in the outer layer of the median eminence;
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2.
neurosecretory material (NSM) in the supraoptico-hypophysial system of the hypothalamus;
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3.
secretory granules in the TSH-cells of the anterior lobe of the hypophysis;
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4.
secretory granules in the ependymal cells of the subcommissural organ (SCO);
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5.
β-cell-granules in the islets of Langerhans in the pancreas.
All these substances are characterized by their stainability with the so-called “Gomorimethod”.
The experiments have included studies into:
-
a)
the extractability of the substances by various solvents;
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b)
the digestability of the substances by pepsin or trypsin;
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c)
their histochemically detectable content of disulfide groups, arginine and periodic acid-Schiff (PAS) reactive carbohydrates;
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d)
their reaction with porcine-neurophysin-II-antibodies.
All substances exhibited a positive reaction for disulfide groups. Based on their solutility properties, their resistance to pepsin or trypsin, their respective content of PAS-reactive carbohydrates and their failure to react with anti-neurophysin serum the “Gomori-positive” granules in TSH-, SCO- and pancreatic β-cells can be distinguished from one another and from CRF- and neurosecretory granules. In contrast, CRF-granules and NSM showed identical properties.
Taking into consideration data from the biochemical and histochemical literature, the present findings suggest that CRF-granules and NSM consist of closely related biochemical substances.
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References
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Dedicated to Professor Dr. Drs. h.c. W. Bargmann on the occasion of his 70th birthday.
This work was supported by grants from the Landesamt für Forschung, Nordrhein-Westfalen (BRD) and from the Medical Research Council of New Zealand.
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Bock, R., Salland, T., Schwabedal, P.E. et al. Histochemical and immunohistochemical properties of the CRF-Granules and other „Gomori-positive” substances of the rat. Histochemistry 46, 81–105 (1976). https://doi.org/10.1007/BF02462734
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DOI: https://doi.org/10.1007/BF02462734