Summary
Receptor-bound hCG was localized in pseudopregnant rat ovarian cells at semiultrastructural level with the peroxidase-antiperoxidase (PAP) complex method. The animals received 2, 6, 12 or 24 h prior to killing a single intravenous injection of hCG and the hormone was localized in the 5-μm paraffin sections and in the 1-μm epon sections using the pre-embedding technique. The peroxidase staining localized to the periphery of the luteal and interstitial glandular cells. No significant staining occurred in the intracellular structures of the cells during the 24-h observation period. However, the appearance of staining in the subplasmalemmal structures can not be excluded. These results are compatible with the previous observations that the receptor-hCG complexes are primarily formed at the surface of the luteal and interstitial glandular cells.
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This work was supported by a grant from The Academy of Finland
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Karjalainen, M., Kinnunen, R., Veijola, M. et al. Localization of receptor-human chrionic gonadotropin complex to the surface of the luteal and interstitial glandular cells in the pseudopregnant rat ovaries with the peroxidase-antiperoxidase (PAP) complex method. Histochemistry 70, 161–166 (1981). https://doi.org/10.1007/BF00493208
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DOI: https://doi.org/10.1007/BF00493208