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Pre-embedding immunohistochemistry as an approach to high resolution antigen localization at the light microscopic level

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Summary

Pre-embedding immunohistochemistry with subsequent embedding in hydroxypropyl methacrylate enables one to obtain high resolution staining of antigens in 1 μ tissue sections. A routine method using formaldehyde fixation, methanol permeation, and an indirect method with fluoresceinlabeled second antibody is described. This method is compared with other pre-embedding staining procedures. To illustrate the method the mouse small intestine was chosen as a model and stained with antibodies to tubulin, actin, and fibronectin. Some anticipated and some unusual staining patterns were found.

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Authors and Affiliations

  1. Department of Structure Biology, Biocenter, University of Basel, CH-4056, Basel, Switzerland

    R. M. Franklin & M. T. Martin

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  1. R. M. Franklin
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  2. M. T. Martin
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Franklin, R.M., Martin, M.T. Pre-embedding immunohistochemistry as an approach to high resolution antigen localization at the light microscopic level. Histochemistry 72, 173–190 (1981). https://doi.org/10.1007/BF00517131

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  • DOI: https://doi.org/10.1007/BF00517131

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