Summary
3H-befunolol was administered intravenously to pigmented rabbits. Thirty minutes after the administration, the iris, ciliary body and retina were fixed, embedded and processed for light microscopic radioautography. Radioautographical silver grains were observed over the pigment granules of the iris, ciliary body, choroid and retina. From these results it is concluded that 3H-befunolol is incorporated into the pigment granules of these cells. The mechanism of incorporation is discussed.
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References
Barbee RF, Smith WO (1957) A comparative study of mydriatic and cycloplegic agents. Am J Ophthalmol 44:617–622
Bénard P, Rico AG, Braun JP, Burgat-Sacazev (1977) New approach to the visualization of receptors by autoradiography. Acta Pharmacol Toxicol 41 (Suppl): 20–21
Bernstein H, Zvaifler N, Rubin M, Mansour AM (1963) The ocular deposition of chloroquine. Invest Ophthalmol 2:384–390
Blois MS (1970) The binding properties of melanin: in vivo and in vitro. Advances in biology of the skin XII. Academic Press, New York, pp 65–79
Chen KK, Poth EJ (1929) Racial difference as illustrated by the mydriatic action of cocaine, euphthalmine and ephedrine. J Pharmacol Exp Ther 36:429–435
Cohen Y (1977) Autoradiographic studies with sympathomimetic amines. Acta Pharmacol Toxicol 61:114–115
Gambill HD, Ogle KN, Kearns TP (1967) Mydriatic effect of four drugs. Arch Ophthalmol 77:740–745
Haddad NJ, Mayer NJ, Riley FC (1970) Mydriatic effect of phenylephrine hydrochloride. Am J Ophthalmol 70:729–734
Hanever WH (1966) Ocular pharmacology. CV Mosby, St. Louis, p 180
Harris LS (1971) Effect of ocular pigmentation on hypotensive response to pilocarpine. Am J Ophthalmol 72:923–925
Hiyama Y (1981) Biochemical analysis on incorporation of 14C-befunolol into melanoma cells in vitro. (in preparation)
Howard HJ, Lee TP (1927) The effect of instillation of ephedrine solution upon the eye. Proc Soc Biol Med 24:700–704
Katz IM, Berger ET (1979) Effect of iris pigmentation on responses of ocular pressure to timolol. Surv Ophthalmol 23:395–398
Kuhn H (1980) Autoradiographic evidence for binding of 3H-flunitrazepan (Rohypnol) to melanin granules in the cat eye. Experientia 36:863–864
Larsson B, Tjälve H (1978) Studies on the melanin affinity of metal ions. Acta Physiol Scand 103:479–484
Luft JH (1977) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414
Melican HE (1977) Ocular pigmentation and pressure and outflow responses to pilocarpine and epinephrine. Am J Ophthalmol 72:70–73
Nagata T, Shibata O, Nawa T (1967) Simplified methods for mass production of radioautographs. Acta Anat Nippon 42:162–166
Nagata T, Murata F (1977) Electron microscopic dry-mounting radioautography for diffusible compounds by means of ultracryotomy. Histochemistry 54:75–82
Potts AM (1964) The reaction of uveal pigment in vitro with polycyclic compounds. Invest Ophthalmol 3:405–416
Reddy NB (1979) Subcellular distribution and characteristics of beta-adrenergic receptors in human skeletal muscle. Neurology 29:566
Scott JG (1945) The eye of the West African Negro. Br J Ophthalmol 29:12–17
Tsukahara S, Yoshida K, Nagata T (1980) Radioautographical study on the incorporation of 14C-bupranolol (beta-blocking agent) into the rabbit eye. Histochemistry 68:237–244
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Yamabayashi, S., Gunarso, W., Tsukahara, S. et al. Incorporation of 3H-befunolol (beta-blocking agent) into melanin granules of ocular tissues in the pigmented rabbits. Histochemistry 73, 371–375 (1981). https://doi.org/10.1007/BF00495651
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DOI: https://doi.org/10.1007/BF00495651