Summary
An attempt was male to identify Lucifer Yellow-labeled neurons in the rat supraoptic nucleus as vasopressin-containing neurons, by means of a combination of immunoperoxidase histochemistry and iontophoretic single cell-injection. We came to the conclusion that the fluorecent dye does not diminish the immunoreactivity of vasopressin in the magnocellular neurons. This newly developed method, along with its modifications, should prove to be quite useful for electrophysiological and morphological studies on the neuropeptide-releasing neurons in the mammalian neuroendocrine system.
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This work was dedicated to Prof. Dr. T.H. Schiebler, Chairman of the Institute of Anatomy of the University of Würzburg, on the occasion of his 60th birthday.
Supported by grants (No. 56370007, 56440022) from the Ministry of Education, Science and Culture, Japan
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Kawata, M., Sano, Y., Inenaga, K. et al. Immunohistochemical identification of Lucifer Yellow-labeled neurons in the rat supraoptic nucleus. Histochemistry 78, 21–26 (1983). https://doi.org/10.1007/BF00491107
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DOI: https://doi.org/10.1007/BF00491107