Skip to main content
SpringerLink
Log in

Cytochemical demonstration of adenylate cyclase activity with cerium

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Cerium was applied for the ultrastructural, cytochemical localization of adenylate cyclase (EC 4.6.1.1.). The enzyme activity was stimulated with norepinephrine, prenalterol and choleratoxin in the brown fat cells of newborn rats. The final reaction product was observed in the plasmalemmas of the stimulated adipocytes. The precipitate was finely crystalline, easily visible in the electron microscope and in the X-ray microprobe analysis it yielded cerium and phosphate peaks, respectively. The use of cerium offers a new tool valid for the cytochemical localization of adenylate cyclase enzyme related to the membrane receptors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bennett V, Cuatrecasas P (1973) Preparation of inverted plasma membrane vesicles from isolated adipocytes. Biochim Biophys Acta 311:362–380

    Google Scholar 

  • Briggs RT, Drath DB, Karnovsky ML, Karnovsky MJ (1975) Localization of NADH oxidase on the cell surface of human polymorphonuclear leucocytes by a new cytochemical method. J Cell Biol 67:566–586

    Google Scholar 

  • Cuatrecases P (1974) Membrane receptors. Annu Rev Biochem 43:169–214

    Google Scholar 

  • Hervonen H, Rechardt L (1976) Histochemical localization of adenylate cyclase in cultured sympathetic neurons. Histochemistry 48:43–50

    Google Scholar 

  • Hervonen H, Rechardt L (1978) Histochemical demonstration of adenylate cyclase activity in the sympathetic ganglion of the chicken. Int J Cell Mol Biol 23:233–240

    Google Scholar 

  • Howell SL, Whitfield M (1972) Cytochemical localization of adenylate cyclase activity in rat islets of Langerhans. J Histochem Cytochem 20:873–879

    Google Scholar 

  • Panula P, Rechardt L (1979) Ultrastructural demonstration of adenylate cyclase activity in the rat neostriatum. Neuroscience 4:779–788

    Google Scholar 

  • Rechardt L, Härkönen M (1975) Electron microscopical demonstration of adenyl cyclase activities in the nervous tissue. Ultrastruct Res 50:365

    Google Scholar 

  • Rechardt L, Härkönen M (1977) Electron microscopical demonstration of adenylate cyclase activity in nervous tissue. Histochemistry 51:113–119

    Google Scholar 

  • Rechardt L, Hervonen H (1976) Electron microscopic localization of adenylate cyclase activity of white and brown adipose tissue of the rat and chicken. Histochemistry 50:57–64

    Google Scholar 

  • Rechardt L, Hervonen H (1981) Electron microscopic demonstration of amine-stimulated adenylate cyclase activity in the adipocyctes. Abstract. 32 Annual Meeting of the American Histochemical Society. Minneapolis, Minnesota. USA

  • Rechardt L, Hervonen H (1982) Effect of norepinephrine, prenalterol and metoprolol on the histochemical localization of adenylate cyclase activity in the brown adipose tissue of newborn rat. Histochemistry 76:489–495

    Google Scholar 

  • Rechardt L, Hervonen H, Panula P (1979) Ultrastructural localization of amine stimulated adenylate cyclase activity. In: Usdin IJ, Kopin, Barchas J (eds) Catecholamines. Basic and clinical frontiers, vol 2. Pergamon Press, New York, pp 1372–1374

    Google Scholar 

  • Rechardt L, Hervonen H, Wojcik W (1984) Ultrastructural demonstration of adenylate cyclase with cerium. VII International Congress of Histochemistry and Cytochemistry, Aug. 5–11, 1984, Helsinki. Abstracts p 351

  • Reik L, Petzold GI, Higgins JA, Greengard P, Barnett RF (1980) Hormone sensitive adenyl cyclase: cytochemical localization in rat liver. Science 168:382–384

    Google Scholar 

  • Robinson JM, Karnovsky MJ (1983a) Ultrastructural localisation of several phosphatases with cerium. J Histochem Cytochem 31:1197–1208

    Google Scholar 

  • Robinson JM, Karnovsky MJ (1983b) Ultrastructural localisation of 5-nucleotidase in guinea pig neutrophils based upon the use of cerium as capturing agent. J Histochem Cytochem 31:1190–1196

    Google Scholar 

  • Schulze W, Hinterberger U, Wollenberger A, Kranes EG, Janiszewski E (1977) Problems of the cytochemical demonstration of adenylate cyclase. Acta Histochem Cytochem 10:371

    Google Scholar 

  • Veenhuis M, Wendelaar Bonga SE (1979) Cytochemical localization of catalase and several hydrogen peroxide-producing oxidases in the nucleoids and matrix of rat liver peroxisomes. Histochem J 11:561–572

    Google Scholar 

  • Vorbrodt AW, Szumanska G, Dobrogowska OH (1984) Cytochemical studies of adenylate cyclase in the choroid plexus and brain vessels of rat and mouse. J Histochem Cytochem 32:275–284

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, University of Tampere, P.O. Box 607, SF-33101, Tampere 10, Finland

    L. Rechardt

  2. Department of Anatomy, University of Helsinki, Siltavuorenpenger 20, SF-00170, Helsinki 17, Finland

    H. Hervonen

Authors
  1. L. Rechardt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Hervonen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This study was supported by the grant from Reino Lahtikari Foundation

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rechardt, L., Hervonen, H. Cytochemical demonstration of adenylate cyclase activity with cerium. Histochemistry 82, 501–505 (1985). https://doi.org/10.1007/BF00489969

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00489969

Keywords

Search

Navigation