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Immunohistochemical demonstration of FC receptors in rat tissues using immune complexes as ligand

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Summary

To demonstrate the presence and localization of Fc receptors, rat liver cryostat sections were incubated with heterologous and autologous immune complexes (ICx) and immunoglobulin (Ig) aggregates. Binding was demonstrated using the immunoperoxidase technique. Autologous and heterologous ICx as well as aggregates from human and rat Ig appeared to bind to the sinusoidal wall. ICx bind in preference to aggregates. Monomeric Ig and aggregated Ig from swine and rabbit did not bind. The results demonstrated that ICx and rat and human Ig aggregates were bound via an Fc receptor. This Fc receptor was still intact in livers from carbontetra chloride and galactosamine treated rats. The receptor could also be demonstrated on spleen macrophages and on kidney interstitial cells. This method turned out to be an useful functional histochemical method to localize Fc receptors and to demonstrate their affinity and species specificity in tissues.

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References

  • Anderson CL, Grey HM (1974) Receptor for aggregated IgG on mouse lymphocytes: Their presence on thymocytes, thymus derived and bone marrow derived lymphocytes. J Exp Med 139:1175–1183

    Google Scholar 

  • Arend WP, Mannik M (1972) In vitro adherence of soluble immune complexes to macrophages. J Exp Med 136:514–529

    Google Scholar 

  • Arend WP, Mannik M (1973) The macrophage receptor for IgG: number and affinity of binding sites. J Immunol 110:1455–1464

    Google Scholar 

  • Askenase PW, Hayden BJ (1974) Cytophilic antibodies in mice contact sensitized with oxazolone. Immunochemical characterization and preferential binding to a trypsin-sensitive macrophage receptor. Immunology 27:563–571

    Google Scholar 

  • Basten A, Miller JFAP, Sprent J, Pye J (1972) A receptor for antibody on B lymphocytes I. J Exp Med 135:610–626

    Google Scholar 

  • Boltz-Nitulescu G, Bazin H, Spiegelberg H (1981) Specificity of Fc receptors for IgG2a, IgG1/IgG2b, and IgG3 on rat macrophages. J Exp Med 154:374–384

    Google Scholar 

  • Decker K, Keppler D (1972) Galactosamine induced liver injury. In: Popper H, Schaffner F (eds) Progress in liver diseases, vol 4. Grune and Stratton, New York, pp 183–199

    Google Scholar 

  • Dickler HB, Kunkel HG (1972) Interaction of aggregated gamma globulin with B lymphocytes. J Exp Med 136:191–195

    Google Scholar 

  • Dower SK, DeLisi C, Titus JA, Segal DM (1981) Mechanism of binding of multivalent immune complexes to Fc receptors. 1. Equilibrium binding. Biochemistry 20:6326–6334

    Google Scholar 

  • Elner VM, Hass AJ, Davis HR, Glagov S (1983) An avidin-biotinperoxidase method for Fc receptors on macrophages isolated from and in sections of rat lung. J Histochem Cytochem 31:1139–1141

    Google Scholar 

  • Froese A, Paraskevas F (1983) (eds) Structure and function of Fc receptors. Marcel Dekker, New York

    Google Scholar 

  • Frommel D, Rachman F (1979) Receptor for the Fc portion of IgG on the plasma membrane of the hepatocyte. Ann Immunol 130C:553–556

    Google Scholar 

  • Gauldie J, Richards C, Lamontagne L (1983) Fc receptors for IgA and other immunoglobulins on resident and activated alveolar macrophages. Mol Immunol 20:1029–1037

    Google Scholar 

  • Gelfland MC, Frank MM, Green I (1979) Binding sites for immune complexes containing IgG in the renal interstitium. Clin Immunol Immunopathol 13:19–28

    Google Scholar 

  • Griffin FM (1980) Effects of soluble immune complexes on Fc receptor and C3b receptor-mediated phagocytosis by macrophages. J Exp Med 152:905–912

    Google Scholar 

  • Groothuis GMM, Weitering JG, Hardonk MJ, Meijer DKF (1983a) Heterogeneity of rat hepatocytes in transport and hepatic binding of asialo-alkaline phosphatase studied after induction of selective acinar damage by N-hydroxy-2-acetylaminofluorene and carbontetrachloride. Biochem Pharmacology 32:2721–2727

    Google Scholar 

  • Groothuis GMM, Meijer DKF, Hardonk MJ (1983b) Morphological studies on selective acinar liver damage by N-hydroxy-2-acetylaminofluorene and carbontetrachloride. Arch Pharmacol 322:298–309

    Google Scholar 

  • Hardonk MJ, Dijkhuis FWJ, Feringa A, Koudstaal J (1982) The development of granulomas in rat liver after i.v. injection of pig intestinal particulate alkaline phosphatase. In: Knook DL, Wisse E (eds) Sinusoidal liver cells. Elsevier Biomedical Press, Amsterdam, pp 185–192

    Google Scholar 

  • Hardonk MJ, Haarsma TJ, Dijkhuis FWJ, Poel M, Koudstaal J (1977) Influence of fixation and buffer treatment on the release of enzymes from the plasma membrane. Histochemistry 54:57–66

    Google Scholar 

  • Heuser CH, Anderson CL, Grey HM (1977) Receptors for IgG: Subclass specificity of receptors on different mouse cell types and the definition of two distinct receptors on a macrophage cell line. J Exp Med 145:1316–1322

    Google Scholar 

  • Itoh G, Miura S, Suzuki I (1977) Immunohistochemical detection of Fc receptors. I: Light microscopic demonstration of Fc receptor by using soluble immune complexes of peroxidase antiperoxidase immunglobulin G. J Histochem Cytochem 25:252–258

    Google Scholar 

  • Jimenez RAH, Mannik M (1982) Evaluation of aggregated IgG in mice as an Fc receptor probe of the hepatic mononuclear phagocyte system. Clin Exp Immunol 49:200–209

    Google Scholar 

  • Jimenez RM, Appay MD, Bellon B, Kuhn J, Bariety J, Druet P (1984) IgG Fc membrane receptor on normal human glomerular visceral epithelial cells. Virchows Arch (Pathol Anat) 404:139–158

    Google Scholar 

  • Kijlstra A (1974) Fate of circulating immune complexes. Thesis, Krips Repro B. V. Meppel, The Netherlands

    Google Scholar 

  • Leslie RGQ (1982) The characterization of cell receptors for IgG. Immunol Today 3, 10:265–267

    Google Scholar 

  • Leslie RGQ, Coupland K (1985) The ingestion and degradation of soluble immune complexes by guinea-pig macrophages and neutrophils. Immunology 54:811–819

    Google Scholar 

  • Leslie RGQ, Niemetz AH (1979) Species specificity in the binding of IgG to macrophages. Immunology 37:835–840

    Google Scholar 

  • Mellman IS (1982) Membrane recycling. Evered D, Collins GM (eds) Ciba Foundation symposium 92. Pitman, London, pp 35–59

    Google Scholar 

  • Mizoguchi Y, Horiuchi Y (1982) Localisation of IgG receptors in human renal glomeruli. Clin Immunol 24:320–329

    Google Scholar 

  • Mizoguchi Y, Tanimoto K, Yue CL, Horiuchi Y (1979) Immune receptors (IgG-Fc and complement receptors) in normal human organs. Lab Invest 40:703–708

    Google Scholar 

  • Mota G, Moraru I, Sjoquist J, Ghetie V (1981) Protein A as a molecular probe for the detection of antigen induced conformational change in Fc region of rabbit antibody. Mol Immunol 18:373–378

    Google Scholar 

  • Petty HR, Hafeman DG, McConnel HM (1980) Specific antibody-dependent phagocytosis of lipid vesicles by RAW264 macrophages results in the loss of cell surface Fc but not C3b receptor activity. J Immunol 125:2391–2398

    Google Scholar 

  • Rasmussen JM, Brandslund I, Leslie RGQ, Svehag SE (1983) Quantitative studies of Fc receptors on human monocytes: Characterisation by binding of homologous and heterologous monomeric IgG and soluble ICx of different composition. Immunology 49:537–544

    Google Scholar 

  • Salmon JE, Kinberly RP, Gibofsky A, Fotino M (1984) Defective mononuclear phagocyte function in systemic lupus erythematosus: Dissociation of Fc receptor-ligand binding and internalization. J Immunol 133:2525–2531

    Google Scholar 

  • Shingu M, Hashimoto Y, Johnson AR, Hurd ER (1981) The search for Fc receptors on human tissues and human endothelial cells in culture. Proc Soc Exp Biol Med 167:147

    Google Scholar 

  • Straus W (1983) Cytochemical observations on mannose-specific binding sites for horse radish peroxidase in liver sinusoidal cells. Histochemistry 77:25–35

    Google Scholar 

  • Thunold S, Schauwenstein K, Wolf H, Thunold KS, Wick G (1981) Localisation of IgG Fc and complement receptors in chicken lymphoid tissue. Scand J Immunol 14:145–153

    Google Scholar 

  • Thunold S, Boyd R, Schauwenstein K, Wick G (1982) Tissue localisation of lymphocyte surface antigens and receptors for immunoglobulin G Fc and complement in the chicken. J Histochem Cytochem 30:201–206

    Google Scholar 

  • Unkeless JC (1977) The presence of two Fc receptors on mouse macrophages: Evidence from a variant cell line and differential trypsin sensitivity. J Exp Med 145:931–947

    Google Scholar 

  • Unkeless JC, Kaplan G, Plutner H, Cohn ZA (1979) Fc receptor variants of a macrophage cell line. Proc Natl Acad Sci USA 76:1400–1411

    Google Scholar 

  • Van der Laan-Klamer SM, Atmosoerodjo-Briggs JE, Harms G, Hardonk MJ, Hoedemaeker PhJ (1985) A histochemical study about the involvement of rat liver cells in the uptake of heterologous immune complexes from the circulation. Histochemistry 82:477–483

    Google Scholar 

  • Van der Laan-Klamer SM, Harms G, Atmosoerodjo-Briggs JE, Hardonk MJ, Hoedemaeker PhJ (1986a) Studies on the mechanism of ICx binding to rat liver cells. Scand J Immunol

  • Van der Laan-Klamer SM, Harms G, Atmosoerodjo-Briggs JE, Hoedemacker PhJ, Hardonk MJ (1986b) Hepatic uptake of autologous immune complexes. Scand J Immunol (in press)

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  1. Department of Pathology, University of Groningen, Oostersingel 63, NL-9713EZ, Groningen, The Netherlands

    S. M. van der Laan-Klamer, G. Harms & M. J. Hardonk

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  1. S. M. van der Laan-Klamer
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  2. G. Harms
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  3. M. J. Hardonk
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van der Laan-Klamer, S.M., Harms, G. & Hardonk, M.J. Immunohistochemical demonstration of FC receptors in rat tissues using immune complexes as ligand. Histochemistry 84, 257–262 (1986). https://doi.org/10.1007/BF00495792

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