Skip to main content
Log in

Immunolocalization of alpha and beta chains of human chorionic gonadotropin, placental lactogen and pregnancy-specific beta-glycoprotein in term placenta by a touch preparation method

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Touch preparations of human placenta yield cells retaining antigenic reactivity to immunoperoxidase stains for α and β chains of human chorionic gonadotropin, placental lactogen, and pregnancy-specific β glycoprotein. This method is a rapid and simple alternative to conventional frozen and paraffin-embedded sections for detection of placental peptides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chard T, Grudzinskas JG (1980) New placental proteins — biology and clinical applications. In: Klopper A, Genazzani A, Crosignani PG (eds) The human placenta: proteins and hormones. Academic Press, London, pp 3–16

    Google Scholar 

  • Chatterjee M, Munro HN (1971) Changing ratio of hCG subunits synthesized by early and full term placental ribosomes. Biochem Biophys Res Commun 77:426–433

    Google Scholar 

  • Currie AR, Beck JS, Ellis S, Read CH (1966) Immunofluorescent localization of growth hormone-like factor in normal and abnormal syncytiotrophoblast. J Pathol Bacteriol 92:395–399

    Google Scholar 

  • de Ikonicoff LK, Cedard L (1973) Localization of human chorionic gonadotropin and somatomammotropic hormones by the peroxidase immunohisto-enzymologic method in villi and amniotic epithelium of human placentas (from six weeks to term). Am J Obstet Gynecol 116:1124–1132

    Google Scholar 

  • Gartner A, Larsson L-I, Sjoberg N-O (1975) Immunohistochemical demonstration of chorionic gonadotropin in trophoblastic tumors. Acta Obstet Gynecol Scand 54:161–163

    Google Scholar 

  • Gau G, Chard T (1975) The distribution of placental lactogen in the human term placenty. Br J Obstet Gynaecol 82:790–793

    Google Scholar 

  • Genbacev O, Sulovic V (1975) Localization of human chorionic gonadotropin (HCG) by immuno-histochemical method in normal and pathological human placenta. Cytobiologie 2:95–100

    Google Scholar 

  • Graham RC Jr, Karnovsky MJ (1976) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302

    Google Scholar 

  • Heyderman E (1978) Multiple tissue markers in human malignant testicular tumours. Scand J Immunol (Suppl) 8:119–126

    Google Scholar 

  • Heyderman E, Gibbons AR, Rosen SW (1981) Immunoperoxidase localization of human placental lactogen: a marker for the placental origin of the giant cells in “syncytial endometritis” of pregnancy. J Clin Pathol 34:303–307

    Google Scholar 

  • Horne CHW, Towler CM, Milne GD (1977) Detection of pregnancy specific β 1-glycoprotein in formalin-fixed tissues. J Clin Pathol 30:19–23

    Google Scholar 

  • Hussa RO, Pattillo RA (1980) Predominance of gonadotropin α-subunit resulting from preferential loss of hCG production in an established cell line. In Vitro 16:585–590

    Google Scholar 

  • Kliman HJ, Nestler JE, Sermasi E, Sanger JM, Strauss JF (1986) Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology 118:1567–1582

    Google Scholar 

  • Lin T-M, Halbert SP (1976) Placental localization of human pregnancy-associated proteins. Science 193:1249–1252

    Google Scholar 

  • Loke YW, Borland R (1970) Immunofluorescent localization of chorionic gonadotropin in monolayer cultures of human trophoblast cells. Nature 228:561–562

    Google Scholar 

  • Midgley AR, Pierce G (1961) Immunohistochemical localization of human chorionic gonadotropin. J Exp Med 115:289–297

    Google Scholar 

  • Morrish DW, Marusyk H, Siy O (1987) Demonstration of specific secretory granules for human chorionic gonadotropin in placenta. J Histochem Cytochem 35:93–101

    Google Scholar 

  • Naughton MA, Merrill DA, McManus LM, Fink LM, Berman E, White MJ, Martinez-Hernandez A (1975) Localization of the chain of human chorionic gonadotropin on human tumor cells and placental cells. Cancer Res 35:1887–1890

    Google Scholar 

  • Sternberger L (1979) Immunocytochemistry. 2nd edn. John Wiley & Sons, New York

    Google Scholar 

  • Tatarinov YS, Falaleeva DM, Kalashnikov VV, Toloknov BO (1976) Immunofluorescent localization of human pregnancy-specific-β1-globulin in placenta and chorioepithelioma. Nature 260:263

    Google Scholar 

  • Yorde DE, Hussa RO, Garancis JC, Patillo RO (1979) Immunocytochemical localization of human choriogonadotropin in human malignant trophoblast. Lab Invest 40:391–398

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morrish, D.W., Manickavel, V., Jewell, L.D. et al. Immunolocalization of alpha and beta chains of human chorionic gonadotropin, placental lactogen and pregnancy-specific beta-glycoprotein in term placenta by a touch preparation method. Histochemistry 88, 57–60 (1987). https://doi.org/10.1007/BF00490168

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00490168

Keywords

Navigation