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Light and electron microscopic detection of (3 Gal β1,4 GlcNAc β1) sequences in asparagine-linked oligosaccharides with the Datura stramonium lectin

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Summary

The Datura stramonium lectin recognizes with high affinity the disaccharide N-acetyllactosamine (Gal β1,4 GlcNAc). We have developed a highly specific cytochemical affinity technique in which an ovomucoid-gold complex serves as second step reagent for the visualization of this lectin bound to reactive sequences present in tissue sections. The lectin binding sites were detected in semithin and ultrathin sections of aldehyde-fixed and low temperature Lowicryl K4M embedded tissues. For light microscopical labeling the photochemical silver reaction for signal amplification was required. The application of this technique for the detection of N-acetyllactosamine containing asparagine-linked oligosaccharides in various intracellular organelles and the plasma membrane is demonstrated.

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Authors and Affiliations

  1. Interdepartmental Electron Microscopy, Biocenter, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland

    C. Egea & J. Roth

  2. Department of Biological Chemistry, University of Michigan, 48109-0606, Ann Arbor, MI, USA

    I. J. Goldstein

Authors
  1. C. Egea
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  2. I. J. Goldstein
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  3. J. Roth
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Additional information

This study was supported by the Swiss National Science Foundation grant nr. 31-26273.89 (to J.R.) and GM 29470 from the National Institutes of Health (to I.J.G.). Dr. G. Egea was a recipient of a European Molecular Biology Organization long term fellowship.

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Egea, C., Goldstein, I.J. & Roth, J. Light and electron microscopic detection of (3 Gal β1,4 GlcNAc β1) sequences in asparagine-linked oligosaccharides with the Datura stramonium lectin. Histochemistry 92, 515–522 (1989). https://doi.org/10.1007/BF00524763

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  • DOI: https://doi.org/10.1007/BF00524763

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