Summary
A new, simple and fast procedure of measuring acrylonitrile (ACN) in ACN derived mercapturic acids such as S-(2-cyanoethyl)-L-cysteine(CyEC), and in hemoglobin (Hb) and plasma protein adducts and urinary metabolites in rats and humans exposed to ACN was developed. ACN in mercapturic acids or proteins was analyzed by capillary gas chromatography (GC) by liberating ACN at a high-temperature in the injector port of GC with or without oxidizing sulfur atoms of the ACN-bound cysteines into sulfoxide form by hydrogen peroxide in vitro. At 350 °C, more than 90% of ACN in authentic CyEC was recovered by this method. Increasing a single ip dose of ACN from 5 to 50 mg/kg produced proportional increases in ACN bound to Hb 24 hr after the treatment. The alkylation of plasma protein with ACN was about 1/10 as low as that of Hb. After repeated daily ip doses of 1–10 mg/kg, ACN in Hb decreased with a half-life of about 9 days. ACN was also detected in the blood of workers exposed to ACN for 1 to 10 years at a Siberian synthetic rubber factory.
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References
ECETOC (1989) DNA and protein adducts: Evaluation of their use in exposure monitoring and risk assessment. In: Monograph No. 13. ECETOC, Brussels, pp 36–42
Fennell TR, MacNeela JP, Turner MJ, Swenberg JA (1989) Hemoglobin adducts formed on administration of acrylonitrile (AN) to rats. Toxicologist 9: 9
Friedman M, Cavins JF, Wall JS (1965) Relative nucleophilic reactivities of amino groups and mercaptide ions in addition reactions with α, β-unsaturated compounds. J Am Chem Soc 87: 3672–3682
Geiger LE, Hogy LL, Guengerich FP (1983) Metabolism of acrylonitrile by isolated rat hepatocytes. Cancer Res 43: 3080–3087
Guengerich FP, Geiger LE, Hogy LL, Wright PL (1981) In vitro metabolism of acrylonitrile to 2-cyanoethylene oxide, reaction with glutathione and irreversible binding to proteins and nucleic acids. Cancer Res 41: 4925–4933
Hashimoto K, Aldridge WN (1970) Biochemical studies on acrylamide, A neurotoxic agent. Biochem Pharmacol 19: 2591–2604
IPCS (1983) Acrylonitrile. Environmental Health Criteria 28. WHO, Geneva, 9–92
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Pereira MA, Chang LW (1980) Binding of chemical carcinogens and mutagens to rat aemoglobin. Chem Biol Interact 33: 301–305
Riggs A (1981) Preparation of blood hemoglobins of vertebrates. In: Antonini E, Rossi-Bemardi L, Chiancone E (eds) Meths Enzymol 76: 5–29
Roberts AE, Kedderis LK, Turner MJ, Richert DE, Swenberg JA (1991) Species comparison of acrylonitrile expoxidation by microsomes from mice, rats and humans: relationship to epoxide concentrations in mouse and rat blood. Carcinogenesis 12: 401–404
Silver EH, Szabo S, Cahill M, Jaeger RJ (1987) Time-course studies of the distribution of [1-14C] acrylonitrile in rats after intravenous administration. J Appl Toxicol 7: 303–306
Tanimoto Y (1989) Hematology. In: Tajima Y (ed) Biological Reference Data Book on Experimental Animals. Soft Science Pub Co, Tokyo, p 96
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Ivanov, V., Hashimoto, K., Inomata, K. et al. Biological monitoring of acrylonitrile exposure through a new analytical approach to hemoglobin and plasma protein adducts and urinary metabolites in rats and humans. Int. Arch Occup Environ Heath 65 (Suppl 1), S103–S106 (1993). https://doi.org/10.1007/BF00381317
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DOI: https://doi.org/10.1007/BF00381317