Summary
In this study we investigated the function of the obstructed small bowel of the rat, the behaviour of the mucosal enzymes, the metabolic changes of the small bowel wall and the morphology of the mucosa. We found a decrease of passive transport of3H-Antipyrine which was equal after 24 and 48 hrs. The active transport of14C-Glucose was found to be progressively inhibited after occlusion. The metabolic enzymes SDH, G-6-PDH, and GOT remained unchanged, LDH was increased after 48 hrs, which can be explained by enzyme induction. The lactate-pyruvate ratio in the tissue of the obstructed bowel was 3 times as high as in the controls. The brush-border enzymes maltase and especially the alcaline phosphatase are decreased with progressive obstruction, which probably is caused by diffusion into the lumen. By electron-microscopy there are no changes in the brush-border membrane but a swelling of mitochondria which is caused by hypoxia.
Zusammenfassung
Bei der mechanischen Dünndarmokklusion der Ratte wurde nach 24 und 48 Std die Absorptionskapazität für14C-Glucose und 3H-Antipyrin gemessen. Es konnte gezeigt werden, daß die Diffusion von Antipyrin nach 24 und 48 Std gleichermaßen eingeschränkt ist, während der aktive Glucosetransport, der nach 24 Std ebenfalls eingeschränkt ist, nach 48 Std weiter verschlechtert ist. Unter den gemessenen Stoffwechselenzymen war die Lactatdehydrogenase nach 48 Std bestehender Okklusion signifikant erhöht. Die Gewebslactatspiegel waren beim Darmverschluß nach 24 Std und 48 Std gegenüber Kontrolltieren auf das 3fache erhöht. Von den Bürstensaumenzymen war die Aktivität der Maltase bei der Okklusion erniedrigt, die alk. Pase zeigte einen signifikanten Abfall, was durch Diffusion ins Darmlumen hervorgerufen wird. Die elektronenoptische Untersuchung der Mucosa ergab Veränderungen der Mitochondrien, die hypoxisch bedingt sind.
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Merkle, P., Bindewald, H. & Breitig, D. Untersuchungen zu Funktion und Stoffwechsel der Mucosa beim mechanischen Dünndarmileus der Ratte. Langenbecks Arch Chiv 338, 291–297 (1975). https://doi.org/10.1007/BF01261718
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DOI: https://doi.org/10.1007/BF01261718