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Untersuchungen zu Funktion und Stoffwechsel der Mucosa beim mechanischen Dünndarmileus der Ratte

Functional and metabolic changes of the mucosa during the occlusion of the small bowel of the rat

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Summary

In this study we investigated the function of the obstructed small bowel of the rat, the behaviour of the mucosal enzymes, the metabolic changes of the small bowel wall and the morphology of the mucosa. We found a decrease of passive transport of3H-Antipyrine which was equal after 24 and 48 hrs. The active transport of14C-Glucose was found to be progressively inhibited after occlusion. The metabolic enzymes SDH, G-6-PDH, and GOT remained unchanged, LDH was increased after 48 hrs, which can be explained by enzyme induction. The lactate-pyruvate ratio in the tissue of the obstructed bowel was 3 times as high as in the controls. The brush-border enzymes maltase and especially the alcaline phosphatase are decreased with progressive obstruction, which probably is caused by diffusion into the lumen. By electron-microscopy there are no changes in the brush-border membrane but a swelling of mitochondria which is caused by hypoxia.

Zusammenfassung

Bei der mechanischen Dünndarmokklusion der Ratte wurde nach 24 und 48 Std die Absorptionskapazität für14C-Glucose und 3H-Antipyrin gemessen. Es konnte gezeigt werden, daß die Diffusion von Antipyrin nach 24 und 48 Std gleichermaßen eingeschränkt ist, während der aktive Glucosetransport, der nach 24 Std ebenfalls eingeschränkt ist, nach 48 Std weiter verschlechtert ist. Unter den gemessenen Stoffwechselenzymen war die Lactatdehydrogenase nach 48 Std bestehender Okklusion signifikant erhöht. Die Gewebslactatspiegel waren beim Darmverschluß nach 24 Std und 48 Std gegenüber Kontrolltieren auf das 3fache erhöht. Von den Bürstensaumenzymen war die Aktivität der Maltase bei der Okklusion erniedrigt, die alk. Pase zeigte einen signifikanten Abfall, was durch Diffusion ins Darmlumen hervorgerufen wird. Die elektronenoptische Untersuchung der Mucosa ergab Veränderungen der Mitochondrien, die hypoxisch bedingt sind.

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Literatur

  1. Crane, R. K.: Absorption of sugars. In: Handbook of Physiology, Section 6, Volume III. Chapter 69 (eds. M. B. Visscher, A. B. Hastings, J. R. Pappenheimer, H. Rahn). American Physiological Society (1968)

  2. Derblom, H., Johansson, H., Nylander, G.: Small intestinal absorption and gastric secretion of iodide in total small bowel obstruction in the rat. Surgery54, 771 (1963)

    PubMed  Google Scholar 

  3. Derblom, H., Johansson, H., Nylander, G.: Vascular pattern of intestinal villi in the obstructed small bowel of the rat. Surgery54, 780 (1963)

    Google Scholar 

  4. Götze, H., Adelson, J. W., Hadorn, H. B., Portmann, R., Troesch, V.: Hormone-elicited enzyme release by the small intestinal wall. Gut18, 471 (1972)

    Google Scholar 

  5. Grace, R. H.: The handling of water and electrolytes by the small bowel following the relief of intestinal obstruction. Brit. J. Surg.58, 760 (1971)

    PubMed  Google Scholar 

  6. Hansed, H. Th., Drube, H. Chr., Klein, U. E., Zielke, K.: Untersuchungen über Muster und Verteilung von Enzymen in der Schleimhaut des Gastrointestinaltraktes. Gastroenterologia(Basel)106, 345 (1966)

    Google Scholar 

  7. Kubrova, J., Robinson, J. W. L., Mirkovitch, V.: La fonction de la muqueuse après une occlusion aigue de l'intestin grêle du rat. Res. exp. Med.160, 321 (1973)

    Google Scholar 

  8. Lowry, O. H., Rosebrough, N. J., Fam, A. L., Randall, R. J.: Protein measurement with the folin-phenol reagent. J. biol. Chem.198, 265 (1951)

    Google Scholar 

  9. Nordström, Chr.: Release of enteropeptidase and other brush-border enzymes from the small intestinal wall in the rat. Biochim. biophys. Acta (Amst.)289, 367 (1972)

    Google Scholar 

  10. Robbins, S. L.: Pathology 3rd Edition. Philadelphia-London: W. B. Saunders Co 1967

    Google Scholar 

  11. Schanker, L. S., Tocco, D. J., Brodie, B. B., Hogben, C. A. M.: Absorption of drugs from the rat small intestine. J. Pharmacol. exp. Ther.123, 81 (1958)

    PubMed  Google Scholar 

  12. Shields, R.: The absorption and secretion of fluid and electrolytes by the obstructed bowel. Brit. J. Surg.52, 774 (1965)

    PubMed  Google Scholar 

  13. Teem, M. V., Phillips, S. F.: Perfusion of the hamster jejunum with conjugated and unconjugated bile acids inhibition of water absorption and effects on morphology. Gastroenterology62, 261 (1972)

    PubMed  Google Scholar 

  14. Varro, V., Csernay, L., Blaho, G., Szarvas, F.: A simple method for the simultaneous recording of blood flow and absorption in isolated segments of dog intestine. Amer. J. dig. Dis.9, 138 (1964)

    Google Scholar 

  15. Wachsmuth, W.: Pathophysiologie und Klinik des Ileus. Langenbecks Arch. klin. Chir.318,143 (1964)

    Google Scholar 

  16. Winne, D.: Durchblutung und enterale Resorption. Z. Gastroent.9, 429 (1971)

    Google Scholar 

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Merkle, P., Bindewald, H. & Breitig, D. Untersuchungen zu Funktion und Stoffwechsel der Mucosa beim mechanischen Dünndarmileus der Ratte. Langenbecks Arch Chiv 338, 291–297 (1975). https://doi.org/10.1007/BF01261718

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  • DOI: https://doi.org/10.1007/BF01261718

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