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Nachweis der DNA-Synthese in Hypocotylen und Cotyledonen gehemmter und nicht gehemmter Keimlinge von Sinapis alba

Evidence of DNA-synthesis in hypocotyls and cytoledons of inhibited and non-inhibited seedlings of Sinapis alba

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Summary

Etiolated elongating hypocotyls of Sinapis alba synthesize DNA 40–72 h after sowing. The increase in DNA content is about 62.5%. Four-hour pulse experiments showed an increasing rate of [32P]orthophosphate incorporation; the maximum rate was obtained 64–68 h after sowing. In the cotyledons DNA increase up to the 56th h after sowing; later the DNA-content remains constant. During this time the incorporation of 32P into the DNA of the cotyledons decreases continuously.

After simultaneous application of Fluordesoxyuridin (FdUrd) (4×10-5M) and thymidine (4×10-4M) or thymidylate (4×10-4M) 40–64 h after sowing, the growth rate was the same as that in H2O treated controls, whereas application of FdUrd (4×10-5M) alone inhibited growth.

In the presence of FdUrd (4×10-5M) thymidine (4×10-4M) and thymidylate (4×10-4M) enhanced the specific activity of the DNA up to respectively 68% and 63% of that in the H2O treated controls. Hydroxyurea (4 mg/ml) inhibited DNA-synthesis and growth in the same manner as FdUrd.

The prolonged DNA-synthesis and the reversal of the FdUrd effect by thymidine or thymidylate are discussed.

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Abbreviations

FdUrd:

Fluordesoxyuridin

dThd:

Thymidin

TMP:

Thymidylat oder Thymidinmonophosphat

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Authors and Affiliations

  1. Botanisches Institut der Universität Heidelberg, Hofmeisterweg 4, D-6900, Heidelberg, Federal Republic of Germany

    I. Capesius & M. Bopp

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  1. I. Capesius
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  2. M. Bopp
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Mit Unterstützung der Deutschen Forschungsgemeinschaft.

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Capesius, I., Bopp, M. Nachweis der DNA-Synthese in Hypocotylen und Cotyledonen gehemmter und nicht gehemmter Keimlinge von Sinapis alba . Planta 118, 171–181 (1974). https://doi.org/10.1007/BF00388392

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  • DOI: https://doi.org/10.1007/BF00388392

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