Summary
Water exchange between the bladder cells of stems of the halophytic species Mesembryanthemum crystallinum and the subepidermal cortical cell layers was determined by direct cell turgor pressure measurements using a pressure probe. Treating the system bladder cell/subepidermal cortex as two homogeneous elastic compartments the hydraulic conductivity, L p, of the barrier separating both compartments was found to be L p=2·10-6 cm ·s-1·bar-1. As discussed, this value essentially reflects the hydraulic conductivity of the bladder cell membrane, which is unusually high compared with the values expected for higher plant cells. The L p-values did not show a dependence on cell turgor pressure over a large pressure range (0.1 to 6 bar) nor on the salinity of the bladder sap (osmolarity range: 500 to 1700 mOsmol). Furthermore, the stationary pressure-values obtained in the pressure-flow experiments point to unusually high permeabilities of the bladder membrane to electrolytes (KCl and NaCl). The unique transport properties of the bladder cell membrane for salt and water suggest a special physiological function of the bladders in the water economy of the plant. It is assumed that the bladders form a reservoir for salts and water in the plant which upon osmotic stress acts as a buffering system to protect the photosynthetic tissue from osmotic injury.
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References
Dainty, J.: Water relations of plant cells. Adv. Bot. Res. 1, 279–326 (1963)
Dainty, J.: The water relations of plants. In: The physiology of plant growth and development (ed. Wilkins, M. B.), p. 421. London: McGraw-Hill 1969
Engmann, K. F.: Studien über die Leistungsfähigkeit der Wassergewebe sukkulenter Pflanzen. Beih. Bot. Zbl. 52, 381–414 (1934)
Epstein, E.: Mineral nutrition of plants: Principles and perspectives. New York-London-Sydney-Toronto: John Wiley 1972
Haberlandt, G.: Physiologische Pflanzenanatomie, 3. Aufl. Leipzig: W. Engelmann 1904
House, C. R.: Water transport in cells and tissues. London: Edward Arnold 1974
Kamiya, N., Tazawa, M., Takata, T.: The relation of turgor pressure to cell volume in Nitella with special reference to mechanical properties of the cell wall. Protoplasma 57, 501–521 (1963)
Kedem, O., Katchalsky, A.: Thermodynamic analysis of the permeability of biological membranes to nonelectrolytes. Biochim. biophys. Acta (Amst.) 27, 229–247 (1958)
Molz, F. J., Ikenberry, E.: Water transport throught plant cells and cell walls. Soil Sci. Soc. Amer. Proc. 38, 699–704 (1974)
Philip, J. R.: The osmotic cell, solute diffusibility, and the plant water economy. Plant Physiol. 33, 264–271 (1958)
Stadelmann, E.: Evaluation of turgidity, plasmolysis and deplasmolysis of plant cells. In: Methods in plant physiology (ed. Prescott, D. M.), Vol. II, p. 143. New York and London: Academic Press Inc. 1966
Steudle, E., Zimmermann, U.: Hydraulische Leitfähigkeit von Valonia utricularis. Z. Naturforsch. 26b, 1302–1311 (1971)
Steudle, E., Zimmermann, U.: Determination of the hydraulic conductivity and of reflection coefficients in Nitella flexilis by means of direct cell-turgor pressure measurements. Biochim. biophys. Acta (Amst.) 332, 399–412 (1974)
Url, W. G.: The site of penetration resistance to water in plant protoplasts. Protoplasma 72, 427–447 (1971)
Walter, H.: Der Wasserhaushalt der Pflanzen in quantitativer Betrachtung. Naturwissenschaft und Landwirtschaft, Heft 6. Freising-München: Datterer u. Cie. 1925
Weatherley, P. E.: Some aspects of water relations. Adv. Bot. Res. 3, 171–206 (1970)
Winter, K.: Evidence for the significance of crassulacean acid metabolism as an adaptive mechanism to water stress. Plant Sci. Letters 3, 279–281 (1974a)
Winter, K.: Einfluß von Wasserstreß auf die Aktivität der Phosphoenolpyruvat-Carboxylase bei Mesembryanthemum crystallinum. Planta (Berl.) 121, 147–153 (1974b)
Winter, K.: Die Rolle des Crassulaceen-Säurestoffwechsels als biochemische Grundlage zur Anpassung von Halophyten an Standorte hoher Salinität. Diss. TH Darmstadt (1975)
Winter, K., Lüttge, U.: Balance between C3 and CAM pathway of photosynthesis. In: Water and plant life: problems and modern approaches (eds. Lange, O. L., Kappen, L., and Schulze, E. D.), part 5B. Berlin-Heidelberg-New York: Springer 1975 (in Press)
Winter, K., Lüttge, U., Ball, E.: 14CO2 dark fication in the halophytic species Mesembryanthemum crystallinum. Biochim. biophys. Acta (Amst.) 343, 465–468 (1974)
Winter, K., v. Willert, D. J.: NaCl-induzierter Crassulaceen-Säurestoffwechsel bei Mesembryanthemum crystallinum. Z. Pflanzenphysiol. 67, 166–170 (1972)
Zimmermann, U., Raede, H., Steudle, E.: Kontinuierliche Druckmessung in Pflanzenzellen. Naturwissenschaften 56, 634 (1969)
Zimmermann, U., Steudle, E.: Hydraulic conductivity and volumetric elastic modulus in giant algal cells: Pressure and volume-dependence, In: Membrane transport in plants (eds. Zimmermann, U. and Dainty, J.), p. 64–71. Berlin-Heidelberg-New York: Springer 1974a
Zimmermann, U., Steudle, E.: The pressure-dependence of the hydraulic conductivity, the membrane resistance and membrane potential during turgor pressure regulation in Valonia utricularis. J. Membrane Biol. 16, 331–352 (1974b)
Zimmermann, U., Steudle, E.: The hydraulic conductivity and volumetric elastic modulus of cells and isolated cell walls of Nitella and Chara: pressure and volume effects. Aust. J. Plant Physiol. 2, 1–12 (1975)
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Steudle, E., Lüttge, U. & Zimmermann, U. Water relations of the epidermal bladder cells of the halophytic species Mesembryanthemum crystallinum: Direct measurements of hydrostatic pressure and hydraulic conductivity. Planta 126, 229–246 (1975). https://doi.org/10.1007/BF00388965
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DOI: https://doi.org/10.1007/BF00388965