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Trace enrichment of cytokinins from Douglas-fir xylem extrudate

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Summary

Quantitative trace-enrichment of cytokinins from a plant source (xylem sap from the Douglasfir, Pseudotsuga menziesii (Mirb.) Franco) has been achieved by adsorption onto octadecyl-silica columns followed by elution with ethanol. Adsorption is rapid and efficient and allows complete recovery of cytokinins at the nanomolar level. Douglas-fir sap contains at least four compounds having cytokinin activity, one of which co-elutes with zeatin and another with ribosylzeatin.

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References

  • Carnes, M.G., Brenner, M.L., Anderson, C.R.: Comparison of reversed phase high-pressure liquid chromatography with Sephadex LH-20 for cytokinin analysis of tomato root pressure exudate. J. Chromat. 108, 95–106 (1975)

    Google Scholar 

  • Fletcher, R.A., McCullagh, D.: Benzyladenine as a regulator of chlorophyll synthesis in cucumber cotyledons. Can. J. Botan. 49, 2197–2201 (1971)

    Google Scholar 

  • Lorenzi, R., Horgan, R., Wareing, P.F.: Cytokinins in Picea sitchensis Carriere: identification and relation to growth. Biochem. Physiol. Pflanzen 168, 333–339 (1975)

    Google Scholar 

  • Robins, M.J., Hall, R.H., Thedford, R.: N6-(Δ 6-Isopentenyl) adenosine. A component of the transfer ribonucleic acid of yeast and of mammalian tissue, methods of isolation, and characterization. Biochem. 6, 1837–1848 (1967)

    Google Scholar 

  • Sweet, G.B., Zaerr, J.B., Lavender, D.P.: The effect of some manipulations on the level of gibberellins in seedling Douglas-fir. In: Mechanisms of Regulation of Plant Growth. Bull. 12, p. 710–713, Bieleski, R.L., Ferguson, A.R., Cresswell, M.M., eds. Wellington: Roy. Soc. New Zealand 1974

    Google Scholar 

  • Thomas, T.H., Carroll, J.E., Isenberg, F.M.R., Pendergrass, A., Howell, L.: Thin-layer chromatography of cytokinins on a mixed layer of polyvinylpyrrolidone and calcium sulfate. J. Chromat. 103, 211–215 (1975a)

    Google Scholar 

  • Thomas, T.H., Carroll, J.E., Isenberg, F.M.R., Pendergrass, A., Howell, L.: A simple, inexpensive, high pressure liquid chromatographic method for separating cytokinins in plant extracts. Plant Physiol. 56, 410–414 (1975b)

    Google Scholar 

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Technical Paper Number 4206, Oregon Agricultural Experiment Station

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Morris, R.O., Zaerr, J.B. & Chapman, R.W. Trace enrichment of cytokinins from Douglas-fir xylem extrudate. Planta 131, 271–274 (1976). https://doi.org/10.1007/BF00385425

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  • DOI: https://doi.org/10.1007/BF00385425

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