Summary
Quantitative trace-enrichment of cytokinins from a plant source (xylem sap from the Douglasfir, Pseudotsuga menziesii (Mirb.) Franco) has been achieved by adsorption onto octadecyl-silica columns followed by elution with ethanol. Adsorption is rapid and efficient and allows complete recovery of cytokinins at the nanomolar level. Douglas-fir sap contains at least four compounds having cytokinin activity, one of which co-elutes with zeatin and another with ribosylzeatin.
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Technical Paper Number 4206, Oregon Agricultural Experiment Station
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Morris, R.O., Zaerr, J.B. & Chapman, R.W. Trace enrichment of cytokinins from Douglas-fir xylem extrudate. Planta 131, 271–274 (1976). https://doi.org/10.1007/BF00385425
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DOI: https://doi.org/10.1007/BF00385425