Abstract
The nuclei of active, sieve cell-associated Strasburger cells in the secondary phloem of Pinus nigra var. austriaca (Hoess) Badoux have been studied for their structure and DNA and RNA content. No difference in size compared to those of ordinary ray cells was found. The nuclear surface is often increased by an ameboid or lobed shape. The amount of highly decondensed chromatin is greatly increased. Cytophotometric measurements of DNA content of both Feulgen and gallocyanine chromalum-stained nuclei showed normal DNA levels and proved absence of endomitotic polyploidization. RNA content, however, was significantly increased as compared to nuclei of young Strasburger cells and of ordinary ray parenchyma cells.
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Abbreviations
- StC1 :
-
Strasburger cells in contact with young and immature sieve cells
- StC2 :
-
Srasburger cells in contact with mature and functionally active sieve cells
- StC3 :
-
dead Strasburger cells
- eRPC pRPC:
-
erect and procumbent ray parenohyma cells, respectively
- GCCA:
-
gallocyanine chromalum
- T:
-
transmission
- A:
-
absorbance
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Dedicated to Professor Dr. Wilhelm Halbsguth, Kiel, on the occation of his 65th birthday
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Sauter, J.J., Ulrich, H. Cytophotometric investigation of DNA and RNA content in nuclei of active Strasburger cells in Pinus nigra var. austriaca (Hoess) Badoux. Planta 137, 5–11 (1977). https://doi.org/10.1007/BF00394427
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DOI: https://doi.org/10.1007/BF00394427