Abstract
Mitochondria from Vicia faba L. exhibit two pathways for the oxidation of endogenous NADH. One pathway is rotenone-sensitive and includes three phosphorylation sites, the other one is rotenone-insensitive and by-passes site I. This by-pass is located in the area of flavoproteins, but several lines of evidence suggest that the rotenone-insensitive oxidation of matrix-NADH is not mediated by the external NADH dehydrogenase. With saturating substrate concentrations the two pathways are superimposed in state 3 while in state 4 the electrons are transferred only via the by-pass. The rotenone-resistant electron flux is obviously regulated from the substrate side. We suggest that the function of the by-pass is to regulate the NADH/NAD+ ratio of the matrix space.
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Abbreviations
- BSA:
-
bovine serum albumin
- CCCP:
-
carbonylcyanide-m-chlorophenylhydrazone
- EDTA:
-
ethylenediaminetetraacetic acid
- HEPES:
-
N-2-hydroxy-ethylpiperazine-ethanesulphonic acid
- PVP:
-
polyvinylpyrrolidone
- TPP:
-
thiaminepyrophosphate
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Marx, R., Brinkmann, K. Characteristics of rotenone-insensitive oxidation of matrix-NADH by broad bean mitochondria. Planta 142, 83–90 (1978). https://doi.org/10.1007/BF00385124
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DOI: https://doi.org/10.1007/BF00385124