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Ultrastructural localization of silver deposits in the receptacle cells of carnation flowers

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Abstract

Carnations were treated with a silver thiosulphate complex to prevent wilting of the flowers. The ultrastructural localization of silver and sulphur in the receptacle tissue was investigated by electron microscopy. Electron-dense deposits were present in the receptacle tissue. Coarse-grained deposts (diam. 60–100 nm) were predominantly observed at the inner side of the cell wall, whereas fine-grained deposits (diam. 20–60 nm) were predominantly present inside the cell-wall region and in the intercellular spaces. These particles were analyzed for chemical elements by X-ray analytical electron microscopy (Philips EM 400 plus Edax energy dispersive analyzer, type 711). In both types of deposits, the presence of silver and sulphur was verified. Point analysis revealed that in both precipitates the S/Ag ratio was of the same order.

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Abbreviations

CTEM:

conventional transmission electron microscope

STEM:

scanning transmission electron microscope

References

  • Abegg, R.: Handbuch der Anorganischen Chemie, vol. II, 1 Leipzig: Hirzel 1908

    Google Scholar 

  • De Filippis, L.F.: Localization of organomercurials in plant cells. Z. Pflanzenphysiol. 88, 133–146 (1978)

    Google Scholar 

  • Findlay, G.P., Pallaghy, C.K.: Potassium chloride in the motor tissue of Stylidium. Aust. J. Plant Physiol. 5, 219–229 (1978)

    Google Scholar 

  • Hall, J.L., Yeo, A.R., Flowers, T.J.: Uptake and localization of rubidium in the halophyte Suaeda maritima. Z. Pflanzenphysiol. 71, 200–206 (1974)

    Google Scholar 

  • Sillén, L.S., Martell, A.E.: Stability constants of metal-ion complexes, Sp. Publ. 17, 2nd edn. London: Chemical Society 1964

    Google Scholar 

  • Van Steveninck, R.F.M., Van Steveninck M.E., Hall, T.A., Peters, P.D.: A chlorine-free embedding medium for use in X-ray analytical electron microscope localization of chloride in biological tissues. Histochemistry 38, 173–180 (1974)

    Google Scholar 

  • Van Steveninck, R.F.M., Ballment, B., Peters, P.D., Hall, T.A.: Ultrastructural localization of ions. II X-ray analytical verification of silver precipitation products and distribution of chloride in mesophyll cells of barley seedlings. Aust. J. Plant Physiol. 3, 359–365 (1976a)

    Google Scholar 

  • Van Steveninck, R.F.M., Van Steveninck, M.E., Peters, P.D., Hall, T.A.: Ultrastructural localization of ions. IV. Localization of chloride and bromide in Nitella translucens and X-ray energy spectroscopy of silver precipitation products. J. Exp. Bot. 27, 1291–1312 (1976b)

    Google Scholar 

  • Veen, H., Van de Geijn, S.C.: Mobility and ionic form of silver as related to longevity of cut carnations. Planta 140, 93–96 (1978)

    Google Scholar 

  • Veen, H.: Effects of silver on ethylene synthesis and action in cut carnations. Planta 145, 467–470 (1979)

    Google Scholar 

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The AEM unit is a joint unit of the Erasmus University of Rotterdam, the University of Leyden, and the Health Organization TNO. The analytical microscope was purchased with a grant from the Dutch Organization for Pure Scientific Research (ZWO) through BION

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Veen, H., Henstra, S. & de Bruyn, W.C. Ultrastructural localization of silver deposits in the receptacle cells of carnation flowers. Planta 148, 245–250 (1980). https://doi.org/10.1007/BF00380034

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  • DOI: https://doi.org/10.1007/BF00380034

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