Abstract
Gibberellic-acid (GA3) treatment of azukibean epicotyls resulted in alterations of the direction of newly deposited microfibrils, on the cell walls. Cells having transverse microfibrils on the inner surface of the wall were observed more frequently in GA3-treated epicotyls than in untreated or water-treated ones. This effect of GA3 was negated by simultaneously supplied colchicine. A crossed polylamellate structure was observed in the inner portion of the walls of GA3-treated cells, but not in the inner portion of the walls of colchicine-treated cells. The wall formed under the influence of colchicine consisted of microfibrils running in the same direction.
Similar content being viewed by others
Abbreviations
- GA:
-
gibberellin
- GA3 :
-
gibberellic acid (gibberellin A3)
References
Deshpande, B.P. (1976) Observations on the fine structure of plant cell walls. I. Use of permanganate staining. Ann. Bot. 40, 433–437
Hepler, P.K., Fosket, D.E. (1971) The role of microtubules in vessel member differentiation in Coleus. Protoplasma 72, 213–236
Hogetsu, T., Shibaoka, H. (1978) Effects of colchicine on cell shape and on microfibril arrangement in the cell wall of Closterium acerosum. Planta 140, 15–18
Hogetsu, T., Shibaoka H., Shimokoriyama, M. (1974a) Involvement of cellulose synthesis in actions of gibberellin and kinetin on cell expansion. Gibberellin-coumarin and kinetin-coumarin interactions on stem elongation. Plant Cell Physiol. 15, 265–272
Hogetsu, T., Shibaika, H., Shimokoriyama, M. (1974b), Involvement of cellulose synthesis in actions of gibberellin and kinetin on cell expansion. 2,6-Dichlorobenzonitrile as a new cellulosesynthesis inhibitor. Plant Cell Physiol. 15, 389–393
Itoh, T. (1976) Microfibrillar orientation of radially enlarged cells of coumarin- and colchicine-treated pine seedlings. Plant Cell Physiol. 17, 385–398
Sawhney, V.K., Srivastave, L.M. (1975) Wall fibrils and microtubules in normal and gibberellic-acid-induced growth of lettuce hypocotyl cells. Can. J. Bot. 53, 824–835
Shibaoka, H. (1972) Gibberellin-colchicine interaction in elongation of azuki bean epicotyl sections. Plant Cell Physiol. 13, 461–469
Shibaoka, H. (1974) Involvement of wall microtubules in gibberellin promotion and kinetin inhibition of stem elongation. Plant Cell Physiol. 15, 255–263
Shibaoka, H., Hogetsu, T. (1977) Effects of ethyl N-phenylcarbamate on wall microtubules and on gibberellin- and kinetincontrolled cell expansion. Bot. Mag. Tokyo 90, 317–321
Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43
Takeda, K., Shibaoka, H. (1978) The fine structure of the epidermal cell wall in azuki bean epicotyl. Bot. Mag. Tokyo 91, 235–245
Takeda, K., Shibaoka, H. (1981) Changes in microfibril arrangement on the inner surface of the epidermal cell walls in the epicotyl of Vigna angularis Ohwi et Obashi during cell growth. Planta 151 385–392
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takeda, K., Shibaoka, H. Effects of gibberellin and colchicine on microfibril arrangement in epidermal cell walls of Vigna angularis Ohwi et Ohashi epicotyls. Planta 151, 393–398 (1981). https://doi.org/10.1007/BF00393297
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00393297