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The effect of monensin on intracellular transport and secretion of α-amylase isoenzymes in barley aleurone

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Abstract

The effect of monensin on the secretion of α-amylase and other enzymes from the aleurone layer of barley (Hordeum vulgare L. cv. Himalaya) was studied by electrophoresis followed by fluorography and by pulse-chase and organelle-isolation experiments. Monensin markedly inhibits the secretion, but not the synthesis, of α-amylase, acid phosphatase, and at least four other proteins from the aleurone layer. Monensin treatment causes α-amylase to accumulate within the protoplast, but its effect on the different α-amylase isoenzymes is not equal. The accumulation of isoenzyme 2 is not influenced by monensin while isoenzymes 1, 3 and 4 are not secreted but rather accumulate in the cell when monensin is included in the incubation medium. The α-amylase and acid-phosphatase activities which accumulate within the aleurone cells following treatment with monensin are localized in an organelle having a buoyant density greater than that of endoplasmic reticulum and less than that of mitochondria. In pulse-chase experiments with [35S]methionine, labelled proteins accumulate in this organelle in the presence of monensin and do not appear in the incubation medium. We conclude that monensin inhibits the secretion of proteins from the barley aleurone layer by influencing their intracellular transport.

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Abbreviations

ER:

endoplasmic reticulum

GA3 :

gibberellic acid

SDS-PAGE:

sodium dodecyl-sulfate polyacrylamide-gel electrophoresis

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Authors and Affiliations

  1. Department of Botany, University of California, 94720, Berkeley, CA, USA

    D. Melroy & R. L. Jones

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  1. D. Melroy
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  2. R. L. Jones
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Melroy, D., Jones, R.L. The effect of monensin on intracellular transport and secretion of α-amylase isoenzymes in barley aleurone. Planta 167, 252–259 (1986). https://doi.org/10.1007/BF00391423

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  • DOI: https://doi.org/10.1007/BF00391423

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