Abstract
The fluence dependence of the time course of accumulation of chalcone synthase mRNA in ultraviolet (UV)-light-irradiated cell suspension cultures of parsley (Petroselinum crispum) and the additional effects of blue and far-red light have been investigated. Variations of the UV fluence had no detectable influence on the initial rate of increase in mRNA amount or translational activity, nor on the preceding lag period of approximately 3 h, but strongly influenced the duration of the transient increase. The effects were the same whether the fluence rate or the time of irradiation was varied to obtain a given fluence. Blue-light pretreatment of the cells resulted in increased amounts of mRNA and abolished the apparent lag period. This effect remained cryptic without the subsequent UV-light treatment. Irradiation with long-wavelength far-red light following UV-light pulses shortened the duration of the mRNA accumulation period. This effect was not altered by a preceding blue-light treatment. Thus, three photoreceptors, a UV-B receptor, a blue-light receptor and phytochrome, participate in the regulation of chalcone synthase mRNA accumulation in this system.
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Abbreviations
- cDNA:
-
complementary DNA
- UV:
-
ultraviolet
- Pfr:
-
fai-red-absorbing form of phytochrome
References
Chappell, J., Hahlbrock, K. (1984) Transcription of plant defense genes in response to UV light or fungal elicitor. Nature 311, 76–78
Duell-Pfaff, N., Wellmann, E. (1982) Involvement of phytochrome and a blue light photoreceptor in UV-B induced flavonoid synthesis in parsley (Petroselinum hortense Hoffm.) cell suspension cultures. Planta 156, 213–217
Drumm H., Mohr, H. (1974) The dose response curve in phytochrome-mediated anthocyanin synthesis in the mustard seedling. Photochem. Photobiol. 20, 151–157
Ebel, J., Hahlbrock, K. (1977) Enzymes of flavone and flavonol-glycoside biosynthesis. Coordinated and selective induction in cell suspension cultures of Petroselinum hortense. Eur. J. Biochem. 75, 201–209
Groner, B., Herrlich, P., Kennedy, N., Ponta, H., Rahmsdorf, V., Heynes, N.E. (1982) Determination of a DNA sequence which confers inducibility of glucocorticoid hormones. J. Cell. Biochem. 20, 349–357
Hahlbrock, K. (1976) Regulation of phenylalanine ammonialyase activity in cell suspension cultures of Petroselinum hortense. Apparent rates of enzyme synthesis and degradation. Eur. J. Biochem. 63, 137–145
Hahlbrock, K., Knobloch, K.-H., Kreuzaler, F., Potts, J.R.M., Wellmann, E. (1976) Coordinated induction and subsequent activity changes in two groups of metabolically interrelated enzymes. Light-induced synthesis of flavonoid glycosides in cell suspension culures of Petroselinum hortense. Eur. J. Biochem. 61, 199–206
Kreuzaler, F., Ragg, H., Fautz, E., Kuhn, D.N., Hahlbrock, K. (1983) UV induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proc. Natl. Acad. Sci. USA 80, 2591–2593
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685
Langridge, P., Pintor-Toro, J.A., Feix, G. (1982) Zein precursor mRNAs from maize endosperm. Mol. Gen. Genet. 187, 432–438
Maccechini, M.L., Rudin, Y., Blobel, G., Schatz, G. (1979) Import of proteins into mitochondria: Precursor forms of extramitochondrially made, F1-ATPase subunits in yeast. Proc. Natl. Acad. Sci. USA 76, 343–347
Oelmüller, R., Mohr, H. (1985) Mode of coaction between blue/UV light and light absorbed by phytochrome in light-mediated anthocyanin formation in the milo (Sorghum vulgare Pers.) seedling. Proc. Natl. Acad. Sci. USA 82, 6124–6128
Pelham, H.R.B., Jackson, R.J. (1976) The reticulocyte lysate. Eur. J. Biochem. 67, 247–256
Reimold, U., Kröger, M., Kreuzaler, F., Hahlbrock, K. (1983) Coding and 3′ non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme. EMBO J. 2, 1801–1805
Schäfer, E., Apel, K., Batschauer, A., Mösinger, E. (1986) The molecular biology of action. In: Photomorphogenesis in plants, Kendrick, R.E., Kronenberg, G.H.M., eds. Dr. W. Junk Publishers, in press
Schröder, J., Heller, W., Hahlbrock, K. (1979a) Flavanone synthase: Simple and rapid assay for the key enzyme of flavonoid biosynthesis. Plant Sci. Lett. 14, 281–286
Schröder, J., Kreuzaler, F., Schäfer, E., Hahlbrock, K. (1979b) Concomitant induction of phenylalanine ammonia-lyase and flavanone synthase mRNAs in irradiated plant cells. J. Biol. Chem. 254, 57–65
Suissa, M. (1983) Spectrophotometric quantitation of silver grains eluted from autoradiograms. Anal. Biochem. 133, 511–514
Wellmann, E. (1975) UV Dose-dependent induction of enzymes related to flavonoid biosynthesis in cell suspension cultures of parsley. FEBS Lett. 51, 105–107
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Bruns, B., Hahlbrock, K. & Schäfer, E. Fluence dependence of the ultraviolet-light-induced accumulation of chalcone synthase mRNA and effects of blue and far-red light in cultured parsley cells. Planta 169, 393–398 (1986). https://doi.org/10.1007/BF00392136
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DOI: https://doi.org/10.1007/BF00392136