Abstract
Influx, efflux and net uptake of NO −3 was studied in Pisum sativum L. cv. Marma in short-term experiments where 13NO −3 was used to trace influx. The influx rate in N-limited plants was similar both during net uptake at external concentrations of around 50 μM, and at low external NO −3 concentrations (4–6 μM) when net uptake was practically zero. Efflux could be inferred from discrepancies between influx and net uptake but was never very high in the N-limited plants during net uptake. Close to the threshold concentration for not NO −3 uptake, efflux was high and equalled influx. Thus, the threshold concentration can be regarded as a NO −3 compensation point. The inclusion of NH +4 in the outer medium decreased influx by about 40% but did not significantly affect efflux. The roles of NO −3 fluxes and nitrate-reductase activity in regulating/limiting NO −3 utilization are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- DW:
-
dry weight
- FW:
-
fresh weight
- RN :
-
relative nitrogen addition rate
References
af Ugglas, M., Larsson, C.-M., Ingemarsson, B., Oscarson, P., Larsson, M., Forsling, W., Iova, I. (1986) The use of 13N in studies of regulation of membrane transport in plants. Bienn. Rep. 1984–1985 of Res. Inst. of Physics, Stockholm, Sweden, 175–181
Belabed, A.M., Pennarum, A.M., Bousquet, U., Convert, M., Cornel, D., Rona, J.P., Monestiez, M. (1986) A study of plasmalemma and tonoplast contribution to NO −3 linked H+ or OH− movements in Acer pseudoplatanus L. cells. 5th Congress of FESPP, 31 August – 4 September 1986, Hamburg, FRG
Belton, P.S., Lee, R.B., Ratcliffe, R.G. (1985) A 14N nuclear magnetic resonance study of inorganic nitrogen metabolism in barley, maize and pea roots. J. Exp. Bot. 36, 190–210
Breteler, H., Nissen, P. (1982) Effect of exogenous and endogenous nitrate concentration on nitrate utilization by dwarf bean. Plant Physiol. 70, 754–759
Clarkson, D.T. (1986) Regulation of the absorption and release of nitrate by plant cells: A review of current ideas and methodology. In: Developments in plant and soil sciences, vol. 19: Fundamental, ecological and agricultural aspects of nitrogen metabolism in higher plants, pp. 3–28, Lambers, H., Neeteson, J.J., Stulen, I., eds. Martinus Nijhoff Publishers, Dordrecht
Clarkson, D.T., Lüttge, U. (1984) Mineral nutrition: Vacuoles and tonoplasts. Progr. in Bot. 46, 56–67
Deane-Drummond, C.E. (1984) Mechanism of nitrate uptake into Chara corallina cells: lack of evidence for obligatory coupling to proton pump and a new NO −3 /NO −3 exchange model. Plant Cell Environ. 7, 317–323
Deane-Drummond, C.E. (1985a) Regulation of nitrate uptake into Chara corallina cells via NH +4 stimulation of NO −3 -efflux. Plant Cell Environ. 8, 105–110
Deane-Drummond, C.E. (1985b) Characteristics of 36ClO −3 and 36Cl− uptake into Pisum sativum L. seedlings: limitations and uses of 36ClO −3 as an analogue for NO −3 . Plant Sci. 42, 99–105
Deane-Drummond, C.E. (1986) Nitrate uptake into Pisum sativum L. cv. Feltham First seedlings: commonality with nitrate uptake into Chara corallina and Hordeum vulgare through a substrate cycling model. Plant Cell Environ. 9, 41–48
Deane-Drummond, C.E., Glass, A.D.M. (1982) Nitrate uptake into barley (Hordeum vulgare) plants. A new approach using 36ClO −3 as an analogue for NO −3 . Plant Physiol. 70, 50–54
Deane-Drummond, C.E., Glass, A.D.M. (1983a) Short-term studies of nitrate uptake into barley plants using ion-specific electrodes and 36ClO −3 . I. Control of net uptake by NO −3 -efflux. Plant Physiol. 73, 100–104
Deane-Drummond, C.E., Glass, A.D.M. (1983b) Short-term studies of nitrate uptake into barley plants using ion-specific electrodes and 36ClO −3 . II. Regulation of NO −3 -efflux by NH +4 . Plant Physiol. 73, 105–110
Drew, M.C., Lee, R.B., Clarkson, D.T. (1986) Effect of plant N-status on kinetics of nitrate influx measured with nitrogen-13. Plant Physiol. 80, Suppl., 89
Ericsson, T., Larsson, C.-M., Tillberg, E. (1982) Growth responses of Lemna to different levels of nitrogen limination. Z. Pflanzenphysiol. 105, 331–340
Gersberg, R., Krohn, K., Peek, N., Goldman, C.R. (1976) Denitrification studies with 13N-labelled nitrate. Science 192, 1229–1231
Glass, A.D.M., Thompson, R.G., Bordeleau, L. (1985) Regulation of NO −3 influx in barley. Studies using 13NO −3 . Plant Physiol. 77, 379–381
Granstedt, R.C., Huffaker, R.C. (1982) Identification of the leaf vacuole as a major nitrate storage pool. Plant Physiol. 70, 410–413
Ingestad, T., Lund, A-B. (1979) Nitrogen stress in birch seedlings. I. Growth Technique and growth. Physiol. Plant. 45, 137–148
Jackson, W.A., Kwik, R.J., Volk, R.J., Butz, R.G. (1976) Nitrate influx and efflux by intact wheat seedlings: Effects of prior nitrate nutrition. Planta 132, 149–156
Lee, R.B., Drew, M.C., Clarkson, D.T. (1985) Nitrate fluxes in barley: studies with 13N. Symp. on Nitrogen Metabolism in Higher Plants, 9–12 April 1985, Groningen, The Netherlands
MacKown, C.T., Jackson, W.A., Volk, R.J. (1982) Restricted nitrate influx and reduction in corn seedlings exposed to ammonium. Plant Physiol. 69, 353–359
Marigo, G., Bouyssou, H., Belkoura, M. (1985) Vacuolar efflux of malata and its influence on nitrogen accumulation in Catharanthus roseus cells. Plant Sci. 39, 97–103
Martinoia, E., Heck, U., Wiemken, A. (1981) Vacuoles as storage compartments for nitrate in barley leaves. Nature 289, 292–294
McNaughton, G.S., Presland, M.R. (1983) Whole plant studies using radioactive 13-nitrogen. I. Techniques for measuring the uptake and transport of nitrate and ammonium ions in hydroponically grown Zea mays. J. Exp. Bot. 34, 880–8921
Morgan, M.A., Jackson, W.A., Volk, R.J. (1985) Concentration dependence of the nitrate assimilation pathway in maize roots. Plant Sci. 38, 185–191
Morgan, M.A., Volk, R.J., Jackson, W.A. (1973) Simultaneous influx and efflux of nitrate during uptake by perennial ryegrass. Plant Physiol. 51, 267–272
Novacky, A., Fisher, E., Ullrich-Eberius, C.I., Lüttge, U., Ullrich, W.R. (1978) Membrane potential changes during transport of glycine as a neutral amino acid and nitrate in Lemna gibba G1. FEBS Lett. 88, 264–267
Oscarson, P., Larsson, C.-M. (1986) Relations between uptake and utilization of NO −3 in Pisum growing exponentially under nitrogen limination. Physiol. Plant. 67, 109–117
Pearson, C.J., Volk, R.J., Jackson, W.A. (1981) Daily changes in nitrate influx, efflux and metabolism in maize and pearl millet. Planta 152, 319–324
Presland, M.R., McNaughton, G.S. (1984) Whole plant studies using radioactive 13-nitrogen. II. A compartmental model for the uptake and transport of nitrate ions by Zea mays. J. Exp. Bot. 35, 1227–1288
Thibaud, J.B., Grignon, C. (1981) Mechanism of nitrate uptake in corn roots. Plant Sci. Lett. 22, 279–289
Ullrich, W.R. (1987) Nitrate and ammonium uptake in green algae and higher plants: Mechanisms and relationship with nitrate metabolism. In: Proc. Adv. Course on Inorganic Nitrogen Metabolism, 22–28 June 1986, Jarandilla, Spain, (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oscarson, P., Ingemarsson, B., af Ugglas, M. et al. Short-term studies of NO −3 uptake in Pisum using 13NO −3 . Planta 170, 550–555 (1987). https://doi.org/10.1007/BF00402990
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00402990