Abstract
The concentration dependences of the binding of fusicoccins (FCs) A, B, C, D, J and H to plasma membranes isolated from maize (Zea mays L.) roots have been studied in parallel with the effects of these compounds on elongation and 86Rb transport in detached maize roots. The dissociation constants obtained showed a good correlation between the affinity of the FCs for the plasmalemma and their biological activity. However, the range of physiologically active FC concentrations proved to be about two orders of magnitude higher than that calculated from the dissociation constants. It was also shown that Vicia faba L. mesophyll protoplasts, unlike isolated plasma membranes, have two FC-binding sites, one with a K D similar to that of the isolated plasmalemma while the other has a substantially higher K D , apparently corresponding to the physiologically active state of the FC-binding proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- FC:
-
fusicoccin
References
Aducci, P., Coletta, M., Marra, M. (1984) An improved Scatchard analysis of fusicoccin-binding to maize coleoptile membranes. Plant. Sci. Lett. 33, 187–193
Aducci, P., Ballio, A., Blein, J.P., Fullone, M.R., Rossignol, M., Scalla, R. (1988) Functional reconstitution of a proton-translocating system responsive to fusicoccin. Proc. Natl. Acad. Sci. USA 85, 7849–7851
Babakov, A.V., Abramycheva, N.Yu. (1989) GTP-binding protein in plasma membranes of higher plants. (In Russ.) Biol. Membrany 6, 262–266
Babakov, A.V., Danilina, E.E. (1990) The comparison study of two types of FC-binding sites localised on the protoplasts plasmalemma of Vicia faba. (In Russ.) Biol. Membrany, in press
Ballio, A. (1977) Fusicoccin: structure-activity relationships. In: Regulation of cell membrane activity in plants, pp. 217–223, Marré, E., Ciferri, O., eds. Elsevier, Amsterdam
Ballio, A., Federico, R., Scalorbi, D. (1981) Fusicoccin structureactivity relationships: in vitro binding to microsomal preparations of maize coleoptiles. Physiol. Plant. 52, 476–481
Beffagna, A., Pesci, P., Tognoli, L., Marré, E. (1979) Distribution of fusicoccin bound in vivo among subcellular fractions from maize coleoptiles. Plant Sci. Lett. 15, 323–330
Blatt, M.R., Clint, G.M. (1989) Mechanism of fusicoccin action: kinetic modification and inactivation of K+ channels in guard cells. Planta 178, 509–523
Blum, W., Hinsh, K.D., Schultz, E.W., Weiler, E.W. (1988) Identification of GTP-binding protein in the plasma membrane of higher plants. Biochem. Biophys. Res. Commun 156, 954–958
De Boyer, A.H., Whatson, B.A., Cleland, R.E. (1989) Purification and identification of the fusicoccin-binding protein from oat root plasma membrane. Plant Physiol. 89, 250–259
Feyeraband, M., Weiler, E.W. (1988) Characterization and localisation of fusicoccin-binding sites in leaf tissues of Vicia faba L. probed with a novel radioligand. Planta 174, 115–122
Feyeraband, M., Weiler, E.W. (1989) Photoaffinity labeling and partial purification of the putative plant receptor for the fungal wilt-inducing toxin, fusicoccin. Planta 178, 282–290
Gilman, G. (1987) G-proteins: transducers of receptor-generated signals. Annu. Rev. Biochem. 56, 615–649
Jacobs, M., Thelen, M.P., Farndale, R.W., Astle, M.C., Rubery, P.H. (1988) Specific guanine nucleotide binding by membrane from Cucurbita pepo seedlings. Biochem. Biophys. Res. Commun. 155, 1478–1484
Marré, E. (1979) Fusicoccin: a tool in plant physiology. Annu. Rev. Plant Physiol. 30, 273–278
Meyer, C., Feyerabend, M., Weiler, E.W. (1989) Fusicoccin-binding proteins in Arabidopsis thaliana (L.) Heynh. Characterization, solubilization and photoaffinity labelling. Plant. Physiol. 89, 692–699
Muromtsev, G.S., Koreneva, V.M., Krasnopol'skaya, L.M. (1980) On some physiological effects of a new plant growth regulator, fusicoccin. (In Russ.) Sel'skokhoz. Biol. 15, 699–703
Stout, R.G. (1988) Fusicoccin activity and binding in Arabidopsis thaliana. Plant Physiol. 88, 999–1001
Varra, F., Serrano, R. (1982) Partial purification and properties of the proton-translocating ATPase of plant plasma membranes. J. Biol. Chem. 257, 12826–12830
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abramycheva, N.Y., Babakov, A.V., Bilushi, S.V. et al. Comparison of the biological activity of fusicoccin in higher plants with its binding to plasma membranes. Planta 183, 315–320 (1991). https://doi.org/10.1007/BF00197727
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00197727