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Plasma-membrane H+-ATPase gene expression is regulated by NaCl in cells of the halophyte Atriplex nummularia L.

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Abstract

An Atriplex nummularia L. cDNA probe encoding the partial sequence of an isoform of the plasma-membrane H+ -ATPase was isolated, and used to characterize the NaCl regulation of mRNA accumulation in cultured cells of this halophyte. The peptide (447 amino acids) translated from the open reading frame has the highest sequence homology to the Nicotiana plumbaginifolia plasma-membrane H+-ATPase isoform pma4 (greater than 80% identity) and detected a transcript of approximately 3.7 kb on Northern blots of both total and poly(A)+ RNA. The mRNA levels were comparable in unadapted cells, adapted cells (cells adapted to and growing in 342 mM NaCl) and deadapted cells (cells previously adapted to 342 mM NaCl that are now growing without salt). Increased mRNA abundance was detected in deadapted cells within 24 h after exposure to NaCl but not in unadapted cells with similar salt treatments. The NaCl up-regulation of message abundance in deadapted cells was subject to developmental control. Analogous to those reported for glycophytes, the plasma-membrane H+-ATPase are encoded by a multigene family in the halophyte.

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Authors and Affiliations

  1. Department of Horticulture, Purdue University, 1165 Horticulture Building, 47907-1165, West Lafayette, IN, USA

    Xiaomu Niu, Jian-Kang Zhu, Meena L. Narasimhan, Ray A. Bressan & Paul M. Hasegawa

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  1. Xiaomu Niu
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  2. Jian-Kang Zhu
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  3. Meena L. Narasimhan
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  4. Ray A. Bressan
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  5. Paul M. Hasegawa
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Additional information

Journal Paper number 13679 of the Purdue University Agricultural Experiment Station. The excellent technical assitance of Jean Clithero and Glenda McClatchey is gratefully appreciated. We also thank Becky Fagan for preparing the manuscript. This research was supported in part by NRICGP grant number 92-37100-7738.

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Niu, X., Zhu, JK., Narasimhan, M.L. et al. Plasma-membrane H+-ATPase gene expression is regulated by NaCl in cells of the halophyte Atriplex nummularia L.. Planta 190, 433–438 (1993). https://doi.org/10.1007/BF00224780

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