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A valine-resistant mutant of Arabidopsis thaliana displays an acetolactate synthase with altered feedback control

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Abstract

A valine-resistant mutant line, VAL-2, of Arabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine and isoleucine. A two fold decrease in apparent K m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine or isoleucine alone. The mutant gene, var1, maps, or is very closely linked, to CSR1, the gene encoding acetolactate synthase in Arabidopsis.

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Abbreviations

ALS:

acetolactate synthase

BCAA:

branched-chain amino acid

CS:

chlorsulfuron

IM:

imidazolinone

SU:

sulfonylurea

TP:

triazolopyrimidine

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Authors and Affiliations

  1. Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Canada

    K. Wu, G. Mourad & J. King

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  1. K. Wu
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  2. G. Mourad
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  3. J. King
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Additional information

We thank Dr. George W. Haughn for providing Arabidopsis lines MSU12, MSU15, MSU21, MSU22 and MSU23. This work was supported by a Research Grant from the Natural Sciences and Engineering Research Council of Canada to J.K., K.W. is grateful for a University of Saskatchewan Graduate Scholarship.

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Wu, K., Mourad, G. & King, J. A valine-resistant mutant of Arabidopsis thaliana displays an acetolactate synthase with altered feedback control. Planta 192, 249–255 (1994). https://doi.org/10.1007/BF00194459

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  • DOI: https://doi.org/10.1007/BF00194459

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