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Structure and expression of cDNAs encoding 1-aminocyclopropane-1-carboxylate oxidase homologs isolated from excised mung bean hypocotyls

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Abstract

By screening a mung bean (Vigna radiata L.) hypocotyl cDNA library using a combination of apple (pAE12) and tomato (pTOM13) 1-aminocyclopropane1-carboxylate (ACC)-oxidase cDNAs as probes, putative ACC-oxidase clones were isolated. Based on restriction-enzyme map and DNA-sequencing analyses, they can be divided into two homology classes, represented by pVR-ACO1 and pVR-ACO2. While pVR-ACO1 and pVR-ACO2 exhibit close homology in their coding regions, their 3′-noncoding regions are divergent. pVR-ACO1 is a 1312-bp full-length clone and contains a single open reading frame encoding 317 amino acids (MW = 35.8 kDa), while pVR-ACO2 is 1172 bp long and is a partial cDNA clone encoding 308 amino acids. These two deduced amino-acid sequences share 83% identity, and display considerable sequence conservation (73–86%) to other ACC oxidases from various plant species. Northern blot analyses of RNAs isolated from hypocotyl, leaf, and stem tissues using gene-specific probes indicate that the pVR-ACO1 transcript is present in all parts of the seedling and that the expression in hypocotyls is further increased following excision. The maximum induction of ACC-oxidase transcripts occurred at about 6 h after excision, while the maximum enzyme activity was observed at 24 h. When excised hypocotyls were treated with ethylene a further enhanced level of transcripts was observed. Aminooxyacetic acid, an inhibitor of ACC-synthase activity, and 2,5-norbornadiene, an inhibitor of ethylene action, suppressed the wound-induced accumulation of ACC-oxidase mRNA, while an addition of ethylene in these tissues restored the accumulation of ACC-oxidase mRNA. These results indicate that the wound-induced expression of ACC-oxidase transcripts is mediated through wound-induced ethylene. Furthermore, when intact mung-bean seedlings were treated with exogenous ethylene, a marked increase in the level of ACC-oxidase mRNA was observed. Together, these results indicate that ethylene plays a key role in activating the expression of the ACC-oxidase gene in both intact and excised mung-bean hypocotyls.

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Abbreviations

ACC:

1-aminocyclopropane-1-carboxylate

AOA:

aminooxyacetic acid

MJ:

methyl jasmonate

NBD:

2,5-norbornadiene

PCR:

polymerase chain reaction

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Author notes

  1. Woo Taek Kim

    Present address: Department of Biology, College of Science, Yonsei University, Seoul, Korea

Authors and Affiliations

  1. Mann Laboratory, Department of Vegetable Crops, University of California, 95616, Davis, CA, USA

    Woo Taek Kim & Shang Fa Yang

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  1. Woo Taek Kim
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  2. Shang Fa Yang
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Additional information

This work was supported by Grant MCB-9303801 from the National Science Foundation. The GenBank accession numbers for the sequences of pVR-ACO1 and pVR-ACO2 are U06046 and U06047, respectively.

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Kim, W.T., Yang, S.F. Structure and expression of cDNAs encoding 1-aminocyclopropane-1-carboxylate oxidase homologs isolated from excised mung bean hypocotyls. Planta 194, 223–229 (1994). https://doi.org/10.1007/BF01101681

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  • DOI: https://doi.org/10.1007/BF01101681

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