Abstract
The elaborate scale case of Mallomonas splendens (Synurophyceae) consists of an overlapping arrangement of siliceous scales. In addition, siliceous bristles are attached to specialized base plate scales located at both the anterior and posterior ends of the cells. We have generated monoclonal antibodies against molecules associated with the scale case of M. splendens. One of these antibodies, designated MsS.H9, labelled a proteinaceous epitope of high-molecular-mass cell surface glycoproteins. Immunofluorescence and immunoelectron microscopy demonstrated that only two regions of M. splendens scale cases were labelled by MsS.H9, namely, the upper surface of the scales that contact neighboring scales and the bases of the bristles. Immunoelectron microscopy using thin sections of M. splendens cells showed these labelling sites corresponded to the amorphous material at the sites of scale-to-scale overlap and to a fibrillar complex located at scale-to-bristle attachment sites. Scales and bristles of M. splendens are formed within the cell, in silica deposition vesicles. Immunolabelling of cell sections containing developing scales and bristles showed that MsS.H9 labelling sites were present very early in the formation of these cell surface components. MsS.H9 labelling was also found associated with developing flagellar hairs whereas no labelling was detected on these structures after their deployment onto the flagellum. The location of MsS.H9 labelling sites strongly suggests that the molecule(s) recognized by the antibody plays a role in the adhesion of the individual components making up the scale case of M. splendens.
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Abbreviations
- CER:
-
chloroplast endoplasmic reticulum
- ER:
-
endoplasmic reticulum
- SDV:
-
silica deposition vesicle
References
Beech PL, Wetherbee R (1990) Direct observations on flagellar transformation in Mallomonas splendens (Synurophyceae). J Phycol 26: 90–95
Beech PL, Wetherbee R, Pickett-Heaps JD (1990) Secretion and deployment of bristles in Mallomonas splendens (Synurophyceae). J Phycol 26: 112–122
Brugerolle G, Bricheux G (1984) Actin filaments are involved in scale formation of the chrysomonad cell Synura. Protoplasma 123: 203–212
Burridge K, Fath K, Kelly T, Nuckolls G, Turner C (1988) Focal adhesions: Transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu Rev Cell Biol 4: 487–525
Cohn SA, Pickett-Heaps JD (1988) The effects of colchicine and dinitrophenol on the in vivo rates of anaphase A and B in the diatom Surirella. Eur J Cell Biol 46: 523–530
Gibbs SP (1981) The chloroplast endoplasmic reticulum: Structure, function, and evolutionary significance. Int Rev Cytol 72: 49–99
Hansen G (1989) Ultrastructure and morphogenesis of scales in Katodinium rotundatum (Lohman) Loeblich (Dinophyceae). Phycologia 28: 385–394
Harlow E, Lane D (1988) Antibodies: A laboratory manual. Cold Spring Harbor Laboratory, New York
Hayman EG, Pierschbacher MD, Suzuki S, Ruoslahti E (1985) Vitronectin-A major cell attachment protein in fetal bovine serum. Exp Cell Res 160: 245–258
Hecky RE, Mopper K, Kilham P, Degens ET (1973) The amino acid and sugar composition of diatom cell-walls. Mar Biol 19: 323–331
Jones JL, Callow JA, Green JR (1990) The molecular nature of Fucus serratus sperm surface antigens recognised by monoclonal antibodies FS1 to FS12. Planta 182: 64–71
Koutoulis A, Ludwig M, Wetherbee R (1993) A scale associated protein of Apedinella radians (Pedinellophyceae) and its possible role in the adhesion of surface components. J Cell Sci 104: 391–398
Kröger N, Bergsdorf C, Sumper M. (1994) A new calcium binding glycoprotein family constitutes a major diatom cell wall component. EMBO J 13: 4676–4683
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Lavau S, Wetherbee R (1994) Structure and development of the scale case of Mallomonas adamas (Synurophyceae). Protoplasma 181: 259–268
Leadbeater BSC (1986) Scale case construction in Synura petersenii Korsch. (Chrysophyceae). In: Kristiansen J, Andersen RA (eds) Chrysophytes: Aspects and problems. Cambridge University Press, Cambridge, pp 121–131
Lind JL, Bacic A, Clarke AE, Anderson MA (1994) A style-specific hydroxyproline-rich glycoprotein with properties of both extensins and arabinogalactan proteins. Plant J 6: 491–502
McGrory CB (1976) A non-siliceous component of chrysophyte scales. Br Phycol J 11: 197
McGrory CB, Leadbeater BSC (1981) Ultrastructure and deposition of silica in the Chrysophyceae. In: Simpson TL, Volcani BE (eds) Silicon and siliceous structures in biological systems. Springer-Verlag, New York, pp 201–230
Meyer DJ, Afonso CL, Galbraith DW (1988) Isolation and characterization of monoclonal antibodies directed against plant plasma membrane and cell wall epitopes: Identification of a monoclonal antibody that recognizes extensin and analysis of the process of epitope biosynthesis in plant tissues and cell cultures. J Cell Biol 107: 163–175
Mignot J-P, Brugerolle G (1982) Scale formation in chrysomonad flagellates. J Ultrastruct Res 81: 13–26
Norman PM, Kjellbom P, Bradley DJ, Hahn MG, Lamb CJ (1990) Immunoaffinity purification and biochemical characterization of plasma membrane arabino-galactan-rich glycoproteins of Nicotiana glutinosa. Planta 181: 365–373
Pickett-Heaps JD, Tippit DH, Andreozzi JA (1979) Cell division in the pennate diatom Pinnularia. IV-Valve morphogenesis. Biol Cell 35: 199–206
Preisig H (1994) Siliceous structures and silicification in flagellated protists. Protoplasma 181: 29–42
Ruoslahti E (1988) Fibronectin and its receptors. Annu Rev Biochem 57: 375–413
Sage EH, Bornstein P (1991) Extracellular matrix proteins that modulate cell-matrix interactions. SPARC, tenascin, and thrombospondin. J Biol Chem 266: 14831–14834
Sanders LC, Wang C-S, Walling LL, Lord EM (1991) A homolog of the substrate adhesion molecule vitronectin occurs in four species of flowering plants. Plant Cell 3: 629–635
Schindler M, Meiners S, Cheresh DA (1989) RGD-dependent linkage between plant cell wall and plasma membrane: Consequences for growth. J Cell Biol 108: 1955–1965
Schnepf E, Deichgräber G (1969) Über die feinstruktur von Synura petersenii unter besonderer Berücksichtigung der Morphogenese ihrer kieselschuppen. Protoplasma 68: 85–106
Showalter AM, Varner JE (1989) Plant hydroxyproline-rich glycoproteins. In: Marcus A (ed) The biochemistry of plants: A comprehensive treatise. Academic Press, New York, vol 15, pp 485–520
Spurr AR (1969) A low-density epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 2: 31–43
Swift DM, Wheeler AP (1992) Evidence of an organic matrix from diatom biosilica. J Phycol 28: 202–209
Wagner VT, Brian L, Quatrano RS (1992) Role of a vitronectin-like molecule in embryo adhesion of the brown alga Fucus. Proc Natl Acad Sci USA 89: 3644–3648
Wang C-S, Walling LL, Gu YQ, Ware CF, Lord EM (1994) Two classes of proteins and mRNAs in Lilium longiflorum L. identified by human vitronectin probes. Plant Physiol 104: 711–717
Wetherbee R, Ludwig M, Koutoulis A (1995) Immunological and ultrastructural studies of scale development and deployment in Mallomonas and Apedinella. In: Sangren CD, Smol JP, Kristiansen J (eds) Chrysophyte algae: Ecology, phylogeny and development. Cambridge University Press, Cambridge, pp 165–178
Wujek DE, Kristiansen J (1978) Observations on bristleand scale-production in Mallomonas caudata (Chrysophyceae). Arch Protistenk 120: 213–221
Zhu J-K, Shi J, Singh U, Wyatt SE, Bressan RA, Hasegawa PM, Carpita NC (1993) Enrichment of vitronectin- and fibronectin-like proteins in NaCl-adapted plant cells and evidence for their involvement in plasma membrane cell wall adhesion. Plant J 3: 637–646
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This work was supported by a grant from the Australian Research Council to R.W. We thank Dr. P. L. Beech for Fig. 13, Dr. L. Perasso for technical assistance and the Plant Cell Biology Group for the use of their monoclonal facilities.
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Ludwig, M., Lind, J.L., Miller, E.A. et al. High molecular mass glycoproteins associated with the siliceous scales and bristles of Mallomonas splendens (Synurophyceae) may be involved in cell surface development and maintenance. Planta 199, 219–228 (1996). https://doi.org/10.1007/BF00196562
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DOI: https://doi.org/10.1007/BF00196562