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Electrophoretic and histochemical analysis of esterase synthesis in the ovariole of the milkweed bugOncopeltus fasciatus (Dall.)

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Summary

The synthesis of esterases was analysed in the ovariole of the milkweed bug using electrophoretic and histochemical methods. We found a total of 12 esterases from whole ovaries. Using the inhibitors p-hydroxymercuribenzoate, diisopropyl phosphorofluoridate and eserine, 3 of the bands were classed as acetylesterases, 7 carboxylesterases, and 2 arylesterases. No change in esterase patterns occurred during oogenesis.

The esterase activity in the follicular epithelium was primarily arylesterase and carboxylesterase. A carboxylesterase was also identified from the central region of the follicle cell. The yolk cells contained mainly carboxylesterase. There was an apparent activation of enzyme activity following pre-treatment with eserine.

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Authors and Affiliations

  1. Department of Zoology, University of Illinois, USA

    P. Cho, R. Davenport & G. S. Whitt

  2. Department of Entomology, University of Illinois, 61801, Urbana, Illinois

    G. M. Booth & J. R. Larsen

  3. Illinois Natural History Survey, Room 163, Natural Resources Building, 61801, Urbana, Illinois

    G. M. Booth

Authors
  1. P. Cho
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  2. R. Davenport
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  3. G. S. Whitt
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  4. G. M. Booth
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  5. J. R. Larsen
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Additional information

This research was supported by NSF-Grant GB 16425 to G.S.W., NSF-Grant GB-14894 to R. D., and Funds from the Biomedical Sciences Committee of the University of Illinois to G.M.B., FWQA Project 16050 EHH to N.H.S., and The Illinois Department of Mental Health Grant No. 258-11-RD to G.M.B.

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Cho, P., Davenport, R., Whitt, G.S. et al. Electrophoretic and histochemical analysis of esterase synthesis in the ovariole of the milkweed bugOncopeltus fasciatus (Dall.). W. Roux' Archiv f. Entwicklungsmechanik 170, 209–220 (1972). https://doi.org/10.1007/BF01380677

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  • DOI: https://doi.org/10.1007/BF01380677

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