Summary
We have visualized segmentation in theDrosophila embryoniccentral nervous system (CNS) by staining for acetylcholinesterase activity. This technique was used to evaluate the effect of ligation on segments in the ventral CNS. When embryos were ligated prior to cellularization (60 min) fewer segmental ganglia developed in posterior fragments than when embryos were ligated at the blatoderm stage (3 h). This result is consistent with previous ligation experiments in which hypodermal segments were counted (Schubiger and Wood 1977). The number of ganglia scored exceeded the number of hypodermal segments scored especially after ligations at 60 min. Possible explanations for this difference are discussed.
References
Fullilove SL, Jacobson AG (1971) Nuclear elongation and cytokinesis inDrosophila montana. Dev Biol 26:560–577
Limbourg B, Zalokar M (1973) Permeabilization ofDrosophila eggs. Dev Biol 35:382–387
Lohs-Schardin M, Cremer C, Nüsslein-Volhard C (1979) A fate map for the larval epidermis ofDrosophila melanogaster: localized cuticle defects following irradiation of the blastoderm with an ultraviolet laser microbeam. Dev Biol 73:239–255
Newman SM Jr, Schubiger G (1980) A morphological and developmental study ofDrosophila embryos ligated during nuclear multiplication. Dev Biol 79:128–138
Pearse AGE (1972) Histochemistry, theoretical, and applied, vol 2. Williams & Wilkins Co, Baltimore, p 1312
Poulson DF (1950) In: Demerec M (ed) Biology ofDrosophila, John Wiley, New York, pp 168–274
Sander K (1971) Pattern formation in longitudinal halves of leaf hopper eggs (Homoptera) and some remarks on the definition of embryonic regulation. Wilhelm Roux's Archives 167:336–352
Schubiger G (1976) Adult differentiation from partialDrosophila embryos after egg ligation during stages of nuclear multiplication and cellular blastoderm. Dev Biol 50:476–488
Schubiger G, Moseley RC, Wood WJ (1977) Interaction of different egg parts in determination of various body regions inDrosophila melanogaster. Proc Natl Acad Sci USA 74:2050–2053
Schubiger G, Wood WJ (1977) Determination during early embryogenesis inDrosophila melanogaster. Am Zool 17:565–576
Turner FR, Mahowald AP (1977) Scanning electron microscopy ofDrosophila melanogaster embryogenesis. II. Gastrulation and segmentation. Dev Biol 57:403–416
Underwood EM, Turner FR, Mahowald AP (1980) Analysis of cell movements and fate mapping during early embryogenesis inDrosophila melanogaster. Dev Biol 74:286–301
Wieschaus E, Gehring W (1976) Clonal analysis of primordial disc cells in the early embryo ofDrosophila melanogaster. Dev Biol 50:249–263
Zalokar M, Erk I (1976) Division and migration of nuclei during early embryogenesis ofDrosophila melanogaster. J Microsc Biol Cell 25:97–106
Zalokar M, Erk I (1977) Phase-partition fixation and staining ofDrosophila eggs. Stain Technol 52:89–95
Zar JH (1974) Biostatistical analysis. Prentice-Hall Inc. Englewood Cliffs NJ
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brown, E.E., Schubiger, G. Segmentation of the central nervous system in ligated embryos ofDrosophila melanogaster . Wilhelm Roux' Archiv 190, 62–64 (1981). https://doi.org/10.1007/BF00868707
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00868707