Summary
Blastomeres isolated from 8-and 16-cell embryos (that is 1/8 and 1/16) show a smooth surface at their point of contact with other blastomeres and a microvillous free surface. Microvilli reappear completely on the smooth surface of 52% of 1/8 embryos and partially on 88% of 1/16 embryos if cultured in vitro for 6 h. When 2-to 8-cell embryos are aggregated to 8-cell embryos and forced apart after 1–3 h, the contact surface of the 8-cell embryos has become smooth. Fixed 8-cell embryos are also able to induce complete disappearance of microvilli on the contact surface of a living 8-cell embryo. Embryos having more than 8 cells do not induce complete disappearance of microvilli on the contact surface of 8-cell embryos. Aggregates of late morulae do not show complete disappearance of microvilli at their contact surfaces but rather a loosening of their peripheral blastomeres.
Our results show that isolated 1/8 and 1/16 embryos tend to recover from regionalization, that the process of aggregation of embryos having 8 cells or less is similar to compaction and that embryos having more than 8 cells seem to aggregate by cell sorting. The processes of compaction, adhesion and reassortment are briefly discussed. We submit that blastomere regionalization, which depends on cell to cell contact, may be the spatial basis of embryonic regulation and of the inside-outside normal differentiation of early mouse embryos.
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References
Biggers JD, Whitten WK, Whittingham DG (1971) The culture of mouse embryos in vitro. In: Daniel JC (ed) Methods in mammalian embryology. Freeman, San Francisco, pp 86–116
Burgoyne PS, Ducibella T (1977) Changes in the properties of the developing trophoblast of preimplantation mouse embryos as revealed by aggregating studies. J Embryol Exp Morphol 40:143–157
Ducibella T (1977) Surface changes of the developing trophoblast cell. In: Johnson MH (ed) Development in mammals, vol 1. North Holland, Amsterdam, pp 5–30
Ducibella T (1980) Divalent antibodies to mouse embryonal carcinoma cells inhibit compaction in the mouse embryo. Dev Biol 79:356–366
Garner W, McLaren A (1974) Cell distribution in chimaeric mouse embryos before implantation. J Embryol Exp Morphol 32:495–503
Grunwald GB, Bronberg REM, Crowley NJ, Lilien J (1981) Enzymatic dissection of embryonic cell adhesive mechanism. II. Developmental regulation of an endogenous adhesive system in the chick neural retina. Dev Biol 86:327–338
Grunwald GB, Pratt RS, Lilien J (1982) Enzymic dissection of embryonic cell adhesive mechanism. III. Immunological identification of a component of the calcium-dependent adhesive system of enbryonic chick neural retina cells. J Cell Sci 55:69–83
Hillman NM, Sherman MI, Graham CF (1972) The effect of spatial arrangement on cell determination during mouse development. J Embryol Exp Morphol 28:263–278
Hyafil F, Babinet C, Jacob F (1981) Cell-cell interactions in early embryogenesis: a molecular approach to the role of calcium. Cell 26:447–454
Izquierdo L, Ebensperger C (1982) Cell membrane regionalization in early mouse embryos as demonstrated by 5′-nucleotidase activity. J Embryol Exp Morphol 69:115–126
Izquierdo L, López T, Marticorena P (1980) Cell membrane regions in preimplantation mouse embryos. J Embryol Exp Morphol 59:89–102
Izquierdo L, López T, Panuncio A (1984) Plasma membrane regionalization and compaction of mouse cleaving embryos: effect of microtubule and microfilament inhibitors. Arch Biol Med Exp 17:29–39
Johnson MH, Ziomek CA (1981) Induction of polarity in mouse 8-cell blastomeres, specificity, geometry and stability. J Cell Biol 91:303–308
Johnson MH, Ziomek CA (1982) Cell subpopulations in the late morula and early blastocyst of the mouse. Dev Biol 91:431–439
Kelly S (1977) Studies of the developmental potential of 4-and 8-cell stage mouse blastomeres. J Exp Zool 200:365–376
Kelly SJ, Mulnard JG, Graham CF (1978) Cell division and cell allocation in early mouse development. J Embryol Exp Morphol 48:37–51
Kimber SJ, Surani MAH (1982) Spreading of blastomeres from 8-cell mouse embryos on lectin coated beads. J Cell Sci 56:191–206
Kimber SJ, Surani MAH, Barton SC (1982) Interactions of blastomeres suggest changes in cell surface adhesiveness during the formation of inner cell mass and trophectoderm in the preimplantation mouse embryo. J Embryol Exp Morphol 70:133–152
Lo CW, Gilula NB (1979) Gap junctional communication in the preimplantation mous embryo. Cell 18:399–409
Magnani JR, Thomas WA, Steinberg MS (1981) Two distinct adhesion mechanisms in embryonic neural retina cells. I. A kinetic analysis. Dev Biol 81:96–105
McLachlin JR, Caveney S, Kidder GM (1983) Control of gap junction formation in early mouse embryos. Dev Biol 98:155–164
Ogou S, Okada TS, Takeichi M (1982) Cleavage stage mouse embryos share a common cell adhesion system with teratocarcinoma cells. Dev Biol 92:521–528
Reeve WJD, Ziomek CA (1981) Distribution of microvilli on dissociated blastomeres from mouse embryos: evidence for surface polarization at compaction. J Embryol Exp Morphol 62:339–350
Roberson M, Armstrong J, Armstrong P (1980) Adhesive and nonadhesive membrane domains of an amphibiam embryo cells. J Cell Sci 44:19–31
Rossant J, Lis WJ (1979) Potential of isolated mouse inner cell masses to form trophectoderm derivatives in vivo. Dev Biol 70:255–261
Rossant J, Vijh KM (1980) Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. Dev Biol 76:475–482
Shirayoshi Y, Okada TS, Takeichi M (1983) The calcium-dependent cell-cell adhesion system regulates inner cell mass formation and cell surface polarization in early mouse development. Cell 35:631–638
Surani MAH, Handyside AH (1983) Reassortment of cells according to position in mouse morulae. J Exp Zool 225:505–511
Takeichi M, Atsumi T, Yoshida C, Uno K, Okada PS (1981) Selective adhesion of embryonal carcinoma cells by Ca++-dependent sites. Dev Biol 87:340–350
Tarkowski AK, Wróblewska J (1967) Development of blastomeres of mouse eggs isolated at the 4-and 8-cell stage. J Embryol Exp Morphol 18:155–180
Thomas WA, Steinberg MS (1981) Two distinct adhesion mechanisms in embryonic neural retina cells. II. An inmunological analysis. Dev Biol 81:106–114
Thomas WA, Thomas J, Magnani JL, Steinberg MS (1981) Two distinct adhesion mechanisms in embryonic neural retina cells. III. Functional specificity. Dev Biol 81:379–385
Yoshida-Noro C, Suzuki N, Takeichi M (1984) Molecular nature of the calcium-dependent cell-cell adhesion system in mouse teratocarcinoma and embryonic cells studied with a monoclonal antibody. Dev Biol 101:19–27
Ziomek CA, Jonson MH (1980) Cell, surface interaction induces polarization of mouse 8-cell blastomeres at compaction. Cell 21:935–942
Ziomek CA, Johnson MH (1981) Properties of polar and apolar cells from the 16-cell mouse morula. Wilhelm Roux's Arch 190:287–296
Ziomek CA, Johnson MH, Handyside AH (1982) The developmental potential of mouse 16-cell blastomeres. J Exp Zool 221:345–355
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Sepúlveda, M.S., Doggenweiler, C. & Izquierdo, L. Scanning microscopy of disaggregated and aggregated preimplantation mouse embryos. Wilhelm Roux' Archiv 194, 445–452 (1985). https://doi.org/10.1007/BF00868145
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DOI: https://doi.org/10.1007/BF00868145