Summary
Eighteen pancreatic neuroendocrine (NE) tumours were analysed for nuclear DNA content by image cytometry (ICM) and flow cytometry (FCM). The DNA indices (DIs) obtained by ICM were somewhat higher than those obtained by FCM, but a major disagreement was present only in 1 case. Thirteen patients had been followed up at least for 6 years after the diagnosis or until death. At 6 years of follow-up all 4 patients with a tumour with a DI≥1.8 by ICM had died from their NE tumour or had metastatic disease, whereas all 9 patients with a smaller DI had no evidence of the disease (P=0.001). The DIs calculated from the FCM data also correlated well with the final outcome (P=0.01). A high incidence of DNA aneuploidy was found by both methods in histologically and clinically benign NE tumours; 12 (67%) were DNA aneuploid by FCM and 16 (89%) by ICM. It is concluded that pancreatic NE tumours are frequently DNA aneuploid, and both cytometric DNA methods give prognostic information in these tumours. The presence of DNA aneuploidy should not be considered as a sign of malignant behaviour in pancreatic NE tumours, whereas a large DI is associated with poor prognosis.
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Alanen KA, Joensuu H, Klemi PJ (1989) Autolysis is a potential source of false aneuploid peaks in flow cytometric DNA histograms. Cytometry 10:417–425
Alanen KA, Joensuu H, Klemi PJ, Martin S, Alavaikko M, Nevalainen TJ (1990) DNA ploidy in pancreatic neuroendocrine tumours. Am J Clin Pathol 93:784–788
Askensten UG, Rosen AK von, Nilsson RS, Auer GU (1989) Intratumoral variations in DNA distribution pattern in mammary adenocarcinomas. Cytometry 10:326–333
Askensten UG, Moberger B, Auer GU (1990) Methodological aspects of cytochemical DNA assessment of adenocarcinoma of the endometrium by means of image and flow cytometry using conventionally formalin-fixed and paraffin-embedded specimens. Arch Geschwulstforsch 60:209–216
Auer G, Askensten U, Ahrens O (1989) Cytophotometry. Hum Pathol 20:518–527
Böhm N, Sandritter W (1975) DNA in human tumors: a cytophotometrix study. Curr Top Pathol 60:151–219
Chejfec G, Falkmer S, Grimelius L, Jacobsson B, Rodensjö M, Wiedenmann B, Franke WW, Lee I, Gould VE (1987) Synaptophysin: a new marker for pancreatic neuroendocrine tumours. Am J Pathol 11:241–247
Donow C, Baisch H, Heitz PU, Klöppel G (1991) Nuclear DNA content in 27 pancreatic endocrine tumours: correlation with malignancy, survival and expression of glycoprotein hormone alpha chain. Virchows Arch [A] 419:463–468
Fallenius A, Auer G, Carstensen J (1988) Prognostic significance of DNA measurement in 409 consecutive breast cancer patients. Cancer 62:331–341
Forsslund G, Zetterberg A (1990) Ploidy level determinations in high-grade and low-grade malignant variants of prostatic carcinoma. Cancer Res 50:4281–4285
Graeme-Cook F, Bell DA, Flotte TJ, Preffer F, Pastel-Levy C, Nardi G, Compton C (1990) Aneuploidy in pancreatic insulinomas does not predict malignancy. Cancer 66:2365–2368
Haugen OA, Mjölneröd O (1990) DNA-ploidy as prognostic factor in prostatic carcinoma. Int J Cancer 45:224–228
Hedley DW (1989) Flow cytometry using paraffin-embedded tissue: five years on. Cytometry 10:229–241
Heitz PU (1984) Pancreatic endocrine tumours. In: Klöppel G, Heitz PU (eds) Pancreatic pathology. Churchill Livingstone, Edinburgh, pp 206–232
Joensuu H, Klemi PJ (1988) DNA aneuploidy in adenoma of endocrine organs. Am J Pathol 132:145–151
Joensuu H, Alanen KA, Klemi PJ (1990) Evidence for false aneuploid peaks in flow cytometric analysis of paraffin-embedded tissue. Cytometry 11:431–437
Klöppel G, Heitz PU (1988) Pancreatic endocrine tumours. Pathol Res Pract 183:155–168
Stipa F, Arganini M, Bibbo M, Straus F, Dytch H, Wied G, Horvath K, Kaplan E (1987) Nuclear DNA analysis of insulinomas and gastrinomas. Surgery 102:988–998
Ullen H, Falkmer UG, Collins VP, Auer GU (1991) Methodologic apsects on nuclear DNA assessment of gliomas with astrocytic and/or olignodendrocytic differentiation. Correlation of image and flow cytometric studies on paraffin-embedded specimen. Anal Quant Cytol Histol 13:168–176
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Alanen, K.A., Falkmer, U.G., Klemi, P.J. et al. Flow and image cytometric study of pancreatic neuroendocrine tumours: Frequent DNA aneuploidy and an association with the clinical outcome. Vichows Archiv A Pathol Anat 421, 121–125 (1992). https://doi.org/10.1007/BF01607044
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DOI: https://doi.org/10.1007/BF01607044