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Myosite ossifiante circonscrite

Histochemical and ultrastructural studies of one case of myositis ossificans circumscripta

Étude histochimique et ultrastructurale d'une observation

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Summary

The authors report a study of myositis ossificans circumscripta in a 62 year old man. The histopathology is briefly described; the histochemistry, histoenzymology and ultrastructure of the 3 types of ossification seen in this lesion (membranous, enchondral and osteoblastic ossification), are reported in detail. The reactions for proteins were positive, and especially those for non-sulfated and sulfated acid M.P.S., and for alkaline phosphatase, esterase, dehydrogenase and leucine-aminopeptidase, in the ossifiable connective matrix. The A.T.P.ase activity was much stronger within the mineralized area of the bone. The conditions of the ossification appeared to be normal, but the ultrastructural study revealed an abnormal character of the collagen of the ossifiable mesenchyme. The collagen fiber was short, its period of 280 to 350 Angstroems, indicating a structural modification, which is possibly genetic and familial in origin. Such a factor may account for the pathological ossification.

Résumé

Les auteurs rapportent une observation de myosite ossifiante circonscrite primitive chez un homme de 62 ans. Après un bref rappel sur l'aspect histopathologique, ils étudient l'histochimie, l'histoenzymologie et l'ultrastructure des 3 types d'ossification rencontrés dans cette lésion (ossification membraneuse, ossification enchondrale, ossification ostéoblastique). Outre l'importance de la positivité des réactions pour les protéines, il faut souligner celle des M.P.S. acides sulfatés et surtout non sulfatés, de l'activité phosphatasique alcaline, estérasique, déshydrogénasique et leucine-amino-peptidasique, au niveau de la matrice conjonctive ossifiable, l'activité A.T.Pasique étant plus grande au niveau de la zone de minéralisation de l'os. Les conditions d'ossification sont sensiblement identiques à celles de l'os normal, mais l'étude ultrastructurale précise le caractère anormal du collagène du mésenchyme ossifiable: celui-ci est un collagène court dont la période est de 280 à 350 Angstrœms. Ce fait constitue une modification de structure — dont l'origine génétique et familiale possible est évoquée — qui pourrait être l'un des facteurs responsables de l'ossification pathologique.

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Authors and Affiliations

  1. Laboratoire d'Anatomie Pathologique, Faculté de Médecine, Reims, France

    T. Caulet, J. J. Adnet, M. Pluot, J. Gougeon & C. Hopfner

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  1. T. Caulet
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  2. J. J. Adnet
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  3. M. Pluot
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  4. J. Gougeon
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  5. C. Hopfner
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Caulet, T., Adnet, J.J., Pluot, M. et al. Myosite ossifiante circonscrite. Virchows Arch. Abt. A Path. Anat. 348, 16–35 (1969). https://doi.org/10.1007/BF00542833

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  • DOI: https://doi.org/10.1007/BF00542833

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