Summary
The Heymann antigen (gp-330) and an antigen with lower molecular weight (gp-90) are major constituents of the brush border of the renal proximal tubules in the rat and the mouse. The Heymann antigen can also be found at discrete sites in the glomerular visceral epithelium of the rat, but not of the mouse. Gp-90 is present diffusely along the glomerular capillary wall of rat and mouse. The Heymann antigen is probably the target antigen for membranous glomerulonephritis in the rat, while in the mouse, where this form of glomerulonephritis can also be induced, gp-90 seems to be the antigen involved. We have separated the antibody populations against these two antigens by preparing eluates from kidneys of rats and livers of mice that had been injected with an antiserum against pronase-digested mouse renal tubular antigens. Using these purified antibodies we have examined by indirect immunofluorescence the distribution of the two antigens on normal mouse and rat tissues. The expression of the Heymann antigen is limited to the epithelia of several organs, while gp-90 has a more widespread distribution in many cells of different origin and function in both the mouse and the rat.
Similar content being viewed by others
References
Assmann KJM, Tangelder MM, Lange WPH, Tadema TM, Koene RAP (1983) Membranous glomerulonephritis in the mouse. Kidney Int 24:303–312
Assmann KJM, Tangelder MM, Lange WPH, Koene RAP (1984) Comparison of the antigenic targets involved in antibody-mediated membranous glomerulonephritis in the mouse and the rat. Kidney Int 25:732 (Abstr)
Assmann KJM, Ronco P, Tangelder MM, Lange WPH, Verroust P, Koene RAP (1985) Comparison of antigenic targets involved in antibody mediatedmembranous glomerulonephritis in the mouse and the rat. Am J Pathol 121:112–122
Bakker WW, Bagchus WM, Hoedemaeker PHJ (1979) Antithymus cell activity of nephritogenic antibodies. Transplantation 27:217–218
Bakker WW, Bagchus WM, Hoedemaeker PHJ (1980) Experimental glomerulonephritis in the rat induced by injection of heterologous antibodies directed to rat thymocytes. In: Preud' homme JL, Hawken VAL (eds). Abstracts of the 4th International Congress of Immunology, International Union of Immunological Societies, Paris
Barabas AZ, Nagi AH, Lannigan R (1970) Induction of autologous immune complex nephritis in rats by heterologous anti-kidney mitochondrial antiserum. Int Urol Nephrol 2:303–308
Chant S, Silverman M (1978) Ontogenic development and tissue distribution of renal proximal tubule brush border membrane antigens in the dog. Clin Exp Immunol 32:405–410
Chant S, Katz A, Silverman M (1980) Pathogenicity of a highly purified brush border membrane preparation in Heymann's nephritis. J Clin Lab Immunol 4:133–140
Doxsey S, Kerjaschki D, Farquhar MG (1983) A large membrane glycoprotein (gp-330) is a resident of coated pits of several absorptive epithelia. J Cell Biol 97:178a (Abstr)
Feenstra K, Van der Lee R, Greben HA, Arends A, Hoedemaeker PHJ (1975) Experimental glomerulonephritis in the rat induced by antibodies directed against tubular antigen. I. The natural history: A histologic and immunohistologic study in the light microscopy and the ultrastructural level. Lab Invest 32:235–242
Fleuren GJ, Grond J, Hoedemaeker PHJ (1980) The pathogenetic role of free circulating antibody in autologous immune complex glomerulonephritis. Clin Exp Immunol 41:205–217
Fukasawa KM, Fukasawa K, Sahara N, Harada M, Kondo Y, Nagatsu I (1981) Immunohistochemical localization of Dipeptidyl Aminopeptidase IV in rat kidney, liver and salivary glands. J Histochem Cytochem 29:337–343
Gossrau R (1979) Peptidasen II. Zur lokalisation der Dipeptidylpeptidase IV (DPP IV). Histochemische und biochemische Untersuchung. Histochemistry 60:231–248
Heymann W, Hackel DB, Harwood S, Wilson SGF, Hunter JLP (1959) Production of nephrotic syndrome in rats by Freund's adjuvants and rat kidney suspensions. Proc Soc Exp Biol Med 100:660–664
Heymann W, Grupe WE, Hunter JLP, Comina A, Hackel DB, Cuppage FE, Weinberg AG (1970) Production of experimental autoimmune nephrosis in rats by heterologous tissues. Nephron 7:363–376
Kenny AJ, Maroux S (1982) Topology of microvillar membrane hydrolyses of kidney and intestine. Physiol Rev 62:91–128
Kerjaschki D, Farquhar MG (1982) The pathogenic antigen of Heymann nephritis is a membrane glycoprotein of the renal proximal tubule brush border. Proc Natl Acad Sci USA 79:5557–5561
Kerjaschki D, Farquhar MG (1983) Immunocytochemical localization of the Heymann antigen (gp-330) in the glomerular epithelial cells of normal Lewis rats. J Exp Med 157:667–686
Kerjaschki D, Noronha-Blob L, Sacktor B, Farquhar MG (1984) Microdomains of distinctive glycoprotein composition in the kidney proximal tubule brush border. J Cell Biol 98:1505–1513
Kerjaschki D, Farquhar MG (1985) Pathogenic antigen of Heymann nephritis (gp-330): Identification, isolation, and localization. In: Robinson RR (ed) Nephrology, vol I, Proceedings of the IXth International Congress of Nephrology, Springer, Berlin Heidelberg New York, p 560
Klassen J, Sugisaki T, Milgrom F, McCluskey RT (1971) Studies on multiple renal lesions in Heymann nephritis. Lab Invest 25:577–585
Linder E (1969) Cross-reacting antigens in the kidney and other organs. Ann Med Exp Fenn 47:55–64
Lowry OH, Rosebrough NJ, Farr RL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Makker SP, Singh AK (1983) Ubiquitous nature of the brush border antigen of Heymann nephritis of rats. Fed Proc 42:512 (Abstr)
Miettinen A, Linder E (1976) Membrane antigens shared by renal proximal tubules and other epithelia associated with absorption and excretion. Clin Exp Immunol 23:568–577
Miettinen A, Törnroth T, Ekblom P, Virtanen I, Linder E (1984) Nephritogenic and non-nephritogenic epithelial antigens in autoimmune and passive Heymann nephritis. Lab Invest 50:435–446
Naruse T (1981) Renal brush border antigens. In: Proceedings of the 8th International Congress of Nephrology, S Karger, Basel, p 891
Ronco P, Allegri L, Melcion C, Pirotsky E, Appay M, Bariety J, Poutillon F, Verroust P (1984a) A monoclonal antibody to brush border and passive Heymann nephritis. Clin Exp Immunol 55:319–332
Ronco P, Melcion C, Geniteau M, Ronco E, Reiniger L, Galceran M, Verroust P (1984b) Production and characterization of monoclonal antibodies against rat brush border antigens of the proximal convoluted tubule. Immunology 53:87–95
Salant DJ, Darby G, Couser WG (1980) Experimental membranous glomerulonephritis in rats. Quantitative studies of glomerular immune deposit formation in isolated glomeruli and whole animals. J Clin Invest 66:71–81
Singh AK, Makker SP (1985) The distribution and molecular presentation of the brush border antigen of Heymann nephritis in various rat tissues. Clin Exp Immunol 60:579–585
Sugisaki T, Klassen K, Andres GA, Milgrom F, McCluskey RT (1973) Passive transfer of Heymann nephritis with serum. Kidney Int 3:66–70
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Assmann, K.J.M., Lange, W.P.H., Tangelder, M.M. et al. The organ distribution of gp-330 (Heymann antigen) and gp-90 in the mouse and the rat. Vichows Archiv A Pathol Anat 408, 541–553 (1986). https://doi.org/10.1007/BF00705307
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00705307